Recombinant Anti-Collagen XVII antibody [EPR14758] (ab186415)
![Immunocytochemistry/ Immunofluorescence - Anti-Collagen XVII antibody [EPR14758] (ab186415)](https://www.abcam.com/ps/products/186/ab186415/Images/ab186415-505675-anti-collagen-XVII-antibody-epr14758-immunocytochemistry-a431-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Collagen XVII antibody [EPR14758] (ab186415)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14758] to Collagen XVII
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Collagen XVII antibody [EPR14758]
See all Collagen XVII primary antibodies -
Description
Rabbit monoclonal [EPR14758] to Collagen XVII -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human skin. ICC/IF: Human A431 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14758 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/250.
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| Notes |
|---|
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/250. |
Target
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Relevance
Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in the gene coding for collagen XVII are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD 1, is generated by proteolytic processing of the full length form. Two transcript variants, one resulting from alternative splicing in the 3' UTR, have been identified for this gene. -
Cellular localization
Cell junction, hemidesmosome. Membrane; Single-pass type II membrane protein. Note=Localized along the plasma membrane of the hemidesmosome. 120 kDa linear IgA disease antigen and 97 kDa linear IgA disease antigen: Secreted, extracellular space, extracellular matrix, basement membrane. -
Database links
- Entrez Gene: 1308 Human
- Omim: 113811 Human
- SwissProt: Q9UMD9 Human
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Alternative names
- 180 kDa bullous pemphigoid antigen 2 antibody
- Alpha 1 type XVII collagen antibody
- BA16H23.2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Collagen XVII antibody [EPR14758] (ab186415)
ab186415 staining Collagen XVII in A431 (Human epidermoid carcinoma epithelial cell). The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% tritonX-100. The cells were then incubated with ab186415 at 10μg/ml concentration followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used at 1/200 dilution as counterstain (shown in red). Secondary antibody only control: PBS instead of the primary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen XVII antibody [EPR14758] (ab186415)](https://www.abcam.com/ps/products/186/ab186415/Images/ab186415-505674-anti-collagen-XVII-antibody-epr14758-immunohistochemistry-skin-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen XVII antibody [EPR14758] (ab186415)Immunohistochemical analysis of Paraffin-embedded sections Human skin tissue labelling Collagen XVII with ab186415 at 1/500 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human skin tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Anti-Collagen XVII antibody [EPR14758] (ab186415)](https://www.abcam.com/ps/products/186/ab186415/Images/ab186415-3-benefits-of-recombinant-antibodies.png)
Anti-Collagen XVII antibody [EPR14758] (ab186415)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (7)
ab186415 has been referenced in 7 publications.
- Watanabe M et al. Type XVII collagen interacts with the aPKC-PAR complex and maintains epidermal cell polarity. Exp Dermatol 30:62-67 (2021). PubMed: 32970880
- Fujimura Y et al. Hair follicle stem cell progeny heal blisters while pausing skin development. EMBO Rep 22:e50882 (2021). PubMed: 34085753
- Kozawa K et al. The CD44/COL17A1 pathway promotes the formation of multilayered, transformed epithelia. Curr Biol 31:3086-3097.e7 (2021). PubMed: 34087104
- Nanba D et al. EGFR-mediated epidermal stem cell motility drives skin regeneration through COL17A1 proteolysis. J Cell Biol 220:N/A (2021). PubMed: 34550317
- Victorelli S et al. Senescent human melanocytes drive skin ageing via paracrine telomere dysfunction. EMBO J 38:e101982 (2019). PubMed: 31633821
- Watanabe M et al. Type XVII collagen coordinates proliferation in the interfollicular epidermis. Elife 6:N/A (2017). PubMed: 28693719
- Barrick BJ et al. Bullous Pemphigoid, Neurodegenerative Disease, and Hippocampal BP180 Expression: A Retrospective Postmortem Neuropathologic Study. J Invest Dermatol 136:2090-2092 (2016). PubMed: 27349860