Anti-COMMD1/MURR1 antibody (ab224727)
Key features and details
- Rabbit polyclonal to COMMD1/MURR1
- Suitable for: IHC-P, IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-COMMD1/MURR1 antibody
See all COMMD1/MURR1 primary antibodies -
Description
Rabbit polyclonal to COMMD1/MURR1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human COMMD1/MURR1 aa 1 to the C-terminus.
Database link: Q8N668 -
Positive control
- WB: A549, Jurkat, MCF7, HeLa and HepG2 whole cell lysate. IP: HepG2 whole cell lysate. IHC-P: Human adrenal gland tissue and liver cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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IP |
1/200 - 1/2000.
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WB |
1/1000 - 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 22, 18 kDa).
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Notes |
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IHC-P
1/20 - 1/200. |
IP
1/200 - 1/2000. |
WB
1/1000 - 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 22, 18 kDa). |
Target
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Function
Proposed scaffold protein that is implicated in diverse physiological processes and whose function may be in part linked to its ability to regulate ubiquitination of specific cellular proteins. Can modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes by displacing CAND1; in vitro promotes CRL E3 activity and dissociates CAND1 from CUL1 and CUL2 (PubMed:21778237). Promotes ubiquitination of NF-kappa-B subunit RELA and its subsequent proteasomal degradation. Down-regulates NF-kappa-B activity (PubMed:15799966, PubMed:17183367, PubMed:20048074). Involved in the regulation of membrane expression and ubiquitination of SLC12A2 (PubMed:23515529). Modulates Na(+) transport in epithelial cells by regulation of apical cell surface expression of amiloride-sensitive sodium channel (ENaC) subunits and by promoting their ubiquitination presumably involving NEDD4L. Promotes the localization of SCNN1D to recycling endosomes (PubMed:14645214, PubMed:20237237, PubMed:21741370). Promotes CFTR cell surface expression through regulation of its ubiquitination (PubMed:21483833). Down-regulates SOD1 activity by interfering with its homodimerization (PubMed:20595380). Plays a role in copper ion homeostasis. Involved in copper-dependent ATP7A trafficking between the trans-Golgi network and vesicles in the cell periphery; the function is proposed to depend on its association within the CCC complex and cooperation with the WASH complex on early endosomes (PubMed:25355947). Can bind one copper ion per monomer (PubMed:17309234). May function to facilitate biliary copper excretion within hepatocytes. Binds to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) (PubMed:18940794). Involved in the regulation of HIF1A-mediated transcription; competes with ARNT/Hif-1-beta for binding to HIF1A resulting in decreased DNA binding and impaired transcriptional activation by HIF-1 (PubMed:20458141). -
Tissue specificity
Ubiquitous. Highest expression in the liver, with lower expression in brain, lung, placenta, pancreas, small intestine, heart, skeletal muscle, kidney and placenta. Down-regulated in cancer tissues. -
Sequence similarities
Contains 1 COMM domain. -
Post-translational
modificationsAcetylated by EP300 ina stimuli-specific manner; protecting it from XIAP-mediated proteasomal degradation and required for interaction with RElA in response to stress.
Ubiquitinated; undergoes both 'Lys-63'- and 'Lys-48'-linked polyubiquitination. Ubiquitinated by XIAP, leading to its proteasomal degradation. -
Cellular localization
Nucleus. Cytoplasm. Endosome membrane. Cytoplasmic vesicle. Early endosome. Recycling endosome. Shuttles between nucleus and cytosol. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. - Information by UniProt
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Database links
- Entrez Gene: 150684 Human
- Omim: 607238 Human
- SwissProt: Q8N668 Human
- Unigene: 468702 Human
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Alternative names
- C2orf5 antibody
- COMD1 antibody
- COMD1_HUMAN antibody
see all
Images
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All lanes : Anti-COMMD1/MURR1 antibody (ab224727) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 22, 18 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted? -
Paraffin-embedded human liver cancer tissue stained for COMMD1/MURR1 with ab224727 at 1/20 dilution in immunohistochemical analysis.
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COMMD1/MURR1 was immunoprecipitated from 0.5 mg of HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab224727 at 1/200 dilution. Western blot was performed from the immunoprecipitate using ab224727 at 1/1000 dilution. HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution.
Lane 1: Rabbit monoclonal IgG instead of ab224727 in HepG2 whole cell lysate.
Lane 2: ab224727 IP in HepG2 whole cell lysate.
Lane 3: HepG2 whole cell lysate 20 μg (Input).
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Paraffin-embedded human adrenal gland tissue stained for COMMD1/MURR1 with ab224727 at 1/20 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab224727 has been referenced in 2 publications.
- Wang J et al. Long non-coding RNA SNHG5 suppresses the development of acute respiratory distress syndrome by targeting miR-205/COMMD1 axis. Mol Cell Biochem 476:1063-1074 (2021). PubMed: 33170429
- Suraweera A et al. COMMD1, from the Repair of DNA Double Strand Breaks, to a Novel Anti-Cancer Therapeutic Target. Cancers (Basel) 13:N/A (2021). PubMed: 33669398