Anti-Complex I subunit 8 kDa antibody [17C8E4E11] (ab110245)
![Western blot - Anti-Complex I subunit 8 kDa antibody [17C8E4E11] (ab110245)](https://www.abcam.com/ps/products/110/ab110245/Images/ab110245-123425-anti-complex-i-subunit-8-kda-antibody-17c8e4e11-western-blot.jpg "Western blot - Anti-Complex I subunit 8 kDa antibody [17C8E4E11] (ab110245)")
Key features and details
- Mouse monoclonal [17C8E4E11] to Complex I subunit 8 kDa
- Suitable for: WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
Overview
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Product name
Anti-Complex I subunit 8 kDa antibody [17C8E4E11] -
Description
Mouse monoclonal [17C8E4E11] to Complex I subunit 8 kDa -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- Isolated mitochondria from Human, Bovine, Rat and Mouse hearts.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purification notes
ab110245 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
17C8E4E11 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. |
Target
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Relevance
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
Cellular localization
Mitochondrial -
Database links
- Entrez Gene: 287027 Cow
- Entrez Gene: 4728 Human
- Entrez Gene: 225887 Mouse
- Entrez Gene: 293652 Rat
- Omim: 602141 Human
- SwissProt: O00217 Human
- SwissProt: Q8K3J1 Mouse
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Alternative names
- BC021616 antibody
- CI 23k antibody
- CI23KD antibody
see all
Images
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Western blot - Anti-Complex I subunit 8 kDa antibody [17C8E4E11] (ab110245)All lanes : Anti-Complex I subunit 8 kDa antibody [17C8E4E11] (ab110245) at 1 µg/ml
Lane 1 : Human heart mitochondria at 10 µg
Lane 2 : Bovine heart mitochondria at 4 µg
Lane 3 : Rat heart mitochondria at 10 µg
Lane 4 : Mouse heart mitochondria at 10 µg
Datasheets and documents
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Datasheet download
References (11)
ab110245 has been referenced in 11 publications.
- Martínez J et al. Mitofusins modulate the increase in mitochondrial length, bioenergetics and secretory phenotype in therapy-induced senescent melanoma cells. Biochem J 476:2463-2486 (2019). PubMed: 31431479
- Philley JV et al. Complex-I Alteration and Enhanced Mitochondrial Fusion Are Associated With Prostate Cancer Progression. J Cell Physiol 231:1364-74 (2016). PubMed: 26530043
- Hauser DN et al. Post-translational decrease in respiratory chain proteins in the Polg mutator mouse brain. PLoS One 9:e94646 (2014). WB ; Mouse . PubMed: 24722488
- Thomas RR et al. Recombinant human mitochondrial transcription factor A stimulates mitochondrial biogenesis and ATP synthesis, improves motor function after MPTP, reduces oxidative stress and increases survival after endotoxin. Mitochondrion 11:108-18 (2011). PubMed: 20727424
- Keeney PM et al. Mitochondrial gene therapy augments mitochondrial physiology in a Parkinson's disease cell model. Hum Gene Ther 20:897-907 (2009). PubMed: 19374590
