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Anti-Complex II Immunocapture antibody [4H12BG12AG2] (ab109865)

Submit a review Q&A (2)References (9)
Immunocytochemistry/ Immunofluorescence - Anti-Complex II Immunocapture antibody [4H12BG12AG2] (ab109865)
  • Immunoprecipitation - Anti-Complex II Immunocapture antibody [4H12BG12AG2] (ab109865)

Key features and details

  • Mouse monoclonal [4H12BG12AG2] to Complex II Immunocapture
  • Suitable for: ICC/IF, IP
  • Reacts with: Cow, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Complex II Immunocapture antibody [4H12BG12AG2]

  • Description

    Mouse monoclonal [4H12BG12AG2] to Complex II Immunocapture

  • Host species

    Mouse

  • Specificity

    Human, bovine, mouse

  • Tested applications

    Suitable for: ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Cow, Human

  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • Bovine heart and Human mitochondria. IF/ICC: HeLa cell line

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 10 µg/ml.
IP
Use at an assay dependent concentration.
Notes
ICC/IF
Use a concentration of 10 µg/ml.
IP
Use at an assay dependent concentration.

Target

  • Relevance

    Complex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma.

  • Cellular localization

    Mitochondrial

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Complex II Immunocapture antibody [4H12BG12AG2] (ab109865)
    Immunocytochemistry/ Immunofluorescence - Anti-Complex II Immunocapture antibody [4H12BG12AG2] (ab109865)

    ICC/IF image of ab109865 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109865, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunoprecipitation - Anti-Complex II Immunocapture antibody [4H12BG12AG2] (ab109865)
    Immunoprecipitation - Anti-Complex II Immunocapture antibody [4H12BG12AG2] (ab109865)

    Complex II immunoprecipitation using antibody ab109865  crosslinked to protein G-agarose beads.  Complex II was immunoprecipitated from a lauryl maltoside detergent extract of 1000 ug bovine heart mitochondria in lane 1 and 500 ug human heart mitochondria in lane 2.  The gel was stained with coomassie brilliant blue.

References (9)

Publishing research using ab109865? Please let us know so that we can cite the reference in this datasheet.

ab109865 has been referenced in 9 publications.

  • Chen T  et al. LGR4 silence aggravates ischemic injury by modulating mitochondrial function and oxidative stress via ERK signaling pathway in H9c2 cells. J Mol Histol 52:363-371 (2021). PubMed: 33559814
  • Zhang Y  et al. Lysine desuccinylase SIRT5 binds to cardiolipin and regulates the electron transport chain. J Biol Chem 292:10239-10249 (2017). PubMed: 28458255
  • Sakakibara I  et al. Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle. Skelet Muscle 6:30 (2016). PubMed: 27597886
  • Jonckheere AI  et al. A complex V ATP5A1 defect causes fatal neonatal mitochondrial encephalopathy. Brain 136:1544-54 (2013). PubMed: 23599390
  • Mendez-Figueroa H  et al. Effects of Labor on Placental Fatty Acid Beta Oxidation. J Matern Fetal Neonatal Med : (2012). Human . PubMed: 22928498
  • Finley LW  et al. Succinate dehydrogenase is a direct target of sirtuin 3 deacetylase activity. PLoS One 6:e23295 (2011). PubMed: 21858060
  • Taanman JW  et al. Characterization of a novel TYMP splice site mutation associated with mitochondrial neurogastrointestinal encephalomyopathy (MNGIE). Neuromuscul Disord 19:151-4 (2009). PubMed: 19056268
  • Nadanaciva S  et al. Target identification of drug induced mitochondrial toxicity using immunocapture based OXPHOS activity assays. Toxicol In Vitro 21:902-11 (2007). PubMed: 17346924
  • Schilling B  et al. Proteomic analysis of succinate dehydrogenase and ubiquinol-cytochrome c reductase (Complex II and III) isolated by immunoprecipitation from bovine and mouse heart mitochondria. Biochim Biophys Acta 1762:213-22 (2006). PubMed: 16120479

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Can this antibody be used for WB?

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Answer

This antibody has been used in western blot in the following publication:

http://jn.nutrition.org/cgi/pmidlookup?view=long&pmid=21346097

We do fully guarantee the antibody to work in WB however, we feel that a better option for running western blots would be ab14715.

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Question

Dear technical team, Hope this e-mail finds you well! Kindly advise the suitable antibodies for below specificities as desired by one of our customer : Target :  Mitochondrial Complex I (encoded by mitochondrial DNA & not nuclear DNA)               Mitochondrial Complex III  (encoded by mitochondrial DNA & not nuclear DNA)Mitochondrial Complex IV  (encoded by mitochondrial DNA & not nuclear DNA) Mitochondrial Complex V   (encoded by mitochondrial DNA & not nuclear DNA)  Applications : IHC & WB Looking forward towards your valued response on the above. With Best Regards,  

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Answer

Thank you for contacting us. Please click these links to find the suitable antibodies. Type the MS code in Abcam search box to get the Abcam catalogue number. Complex I http://www.mitosciences.com/complex_i.html Complex IV http://www.mitosciences.com/complex_iv.html Complex III http://www.mitosciences.com/complex_iii.html Complex II http://www.mitosciences.com/complex_ii.html We also have Immuno capture kits available https://www.abcam.com/index.html?pageconfig=resource&rid=13917 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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