Anti-Complex II Immunocapture antibody [4H12BG12AG2] (ab109865)
Key features and details
- Mouse monoclonal [4H12BG12AG2] to Complex II Immunocapture
- Suitable for: ICC/IF, IP
- Reacts with: Cow, Human
- Isotype: IgG1
Overview
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Product name
Anti-Complex II Immunocapture antibody [4H12BG12AG2] -
Description
Mouse monoclonal [4H12BG12AG2] to Complex II Immunocapture -
Host species
Mouse -
Specificity
Human, bovine, mouse -
Tested applications
Suitable for: ICC/IF, IPmore details -
Species reactivity
Reacts with: Cow, Human -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- Bovine heart and Human mitochondria. IF/ICC: HeLa cell line
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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Purification notes
ab109865 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
4H12BG12AG2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 10 µg/ml.
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IP |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
IP
Use at an assay dependent concentration. |
Target
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Relevance
Complex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma. -
Cellular localization
Mitochondrial
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Complex II Immunocapture antibody [4H12BG12AG2] (ab109865)
ICC/IF image of ab109865 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109865, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Complex II immunoprecipitation using antibody ab109865 crosslinked to protein G-agarose beads. Complex II was immunoprecipitated from a lauryl maltoside detergent extract of 1000 ug bovine heart mitochondria in lane 1 and 500 ug human heart mitochondria in lane 2. The gel was stained with coomassie brilliant blue.
Protocols
Datasheets and documents
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Datasheet download
References (9)
ab109865 has been referenced in 9 publications.
- Chen T et al. LGR4 silence aggravates ischemic injury by modulating mitochondrial function and oxidative stress via ERK signaling pathway in H9c2 cells. J Mol Histol 52:363-371 (2021). PubMed: 33559814
- Zhang Y et al. Lysine desuccinylase SIRT5 binds to cardiolipin and regulates the electron transport chain. J Biol Chem 292:10239-10249 (2017). PubMed: 28458255
- Sakakibara I et al. Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle. Skelet Muscle 6:30 (2016). PubMed: 27597886
- Jonckheere AI et al. A complex V ATP5A1 defect causes fatal neonatal mitochondrial encephalopathy. Brain 136:1544-54 (2013). PubMed: 23599390
- Mendez-Figueroa H et al. Effects of Labor on Placental Fatty Acid Beta Oxidation. J Matern Fetal Neonatal Med : (2012). Human . PubMed: 22928498
- Finley LW et al. Succinate dehydrogenase is a direct target of sirtuin 3 deacetylase activity. PLoS One 6:e23295 (2011). PubMed: 21858060
- Taanman JW et al. Characterization of a novel TYMP splice site mutation associated with mitochondrial neurogastrointestinal encephalomyopathy (MNGIE). Neuromuscul Disord 19:151-4 (2009). PubMed: 19056268
- Nadanaciva S et al. Target identification of drug induced mitochondrial toxicity using immunocapture based OXPHOS activity assays. Toxicol In Vitro 21:902-11 (2007). PubMed: 17346924
- Schilling B et al. Proteomic analysis of succinate dehydrogenase and ubiquinol-cytochrome c reductase (Complex II and III) isolated by immunoprecipitation from bovine and mouse heart mitochondria. Biochim Biophys Acta 1762:213-22 (2006). PubMed: 16120479