Anti-Complex III Immunocapture antibody [11A51H12] (ab109862)
![Immunoprecipitation - Anti-Complex III Immunocapture antibody [11A51H12] (ab109862)](https://www.abcam.com/ps/products/109/ab109862/Images/ab109862-122853-anti-complex-iii-immunocapture-antibody-11a51h12-immunoprecipitation.jpg "Immunoprecipitation - Anti-Complex III Immunocapture antibody [11A51H12] (ab109862)")
Key features and details
- Mouse monoclonal [11A51H12] to Complex III Immunocapture
- Suitable for: IP
- Reacts with: Cow, Human
- Isotype: IgG1
Overview
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Product name
Anti-Complex III Immunocapture antibody [11A51H12] -
Description
Mouse monoclonal [11A51H12] to Complex III Immunocapture -
Host species
Mouse -
Specificity
100 µg ab109862 can capture at least 10 µg Complex III from 1 mg solubilized bovine heart mitochondria. -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Cow, Human -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- Bovine heart and Human mitochondria
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purification notes
ab10862 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
11A51H12 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at 100 µg/mg of lysate.
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| Notes |
|---|
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IP
Use at 100 µg/mg of lysate. |
Target
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Relevance
Complex III, or ubiquinol cytochrome c oxidoreductase, is a complex of 11 subunits and catalyses electron transfer from reduced ubiquinone or coenzyme Q 10 to cytochrome c. The complex contains 11 different subunits, one of which, cytochrome b, is encoded on mitochondrial DNA, while the remainder subunits are nuclear-encoded. Genetic complex III deficiencies occur but are rare.
Images
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Immunoprecipitation - Anti-Complex III Immunocapture antibody [11A51H12] (ab109862)Staining of Complex III Lane 1: 0.1mg of Bovine mitochondria Lane 2: 0.05mg Bovine mitochondria Lane 3: 0.05mg Human mitochondria Immunoprecipitated using ab109862 at 10 µg/mg lysate.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab109862 has been referenced in 8 publications.
- Chen T et al. LGR4 silence aggravates ischemic injury by modulating mitochondrial function and oxidative stress via ERK signaling pathway in H9c2 cells. J Mol Histol 52:363-371 (2021). PubMed: 33559814
- Thiriveedi VR et al. Glutathionylated and Fe-S cluster containing hMIA40 (CHCHD4) regulates ROS and mitochondrial complex III and IV activities of the electron transport chain. Redox Biol 37:101725 (2020). PubMed: 32971361
- Beutner G et al. Cyclophilin D regulates the dynamic assembly of mitochondrial ATP synthase into synthasomes. Sci Rep 7:14488 (2017). PubMed: 29101324
- Beutner G et al. Initiation of electron transport chain activity in the embryonic heart coincides with the activation of mitochondrial complex 1 and the formation of supercomplexes. PLoS One 9:e113330 (2014). IP ; Mouse . PubMed: 25427064
- Jonckheere AI et al. A complex V ATP5A1 defect causes fatal neonatal mitochondrial encephalopathy. Brain 136:1544-54 (2013). PubMed: 23599390
- Dumas de La Roque E et al. DHEA reverses chronic hypoxia/reoxygenation-induced right ventricular dysfunction in rats. Eur Respir J : (2012). WB . PubMed: 22523357
- Gusarova GA et al. Hypoxia leads to Na,K-ATPase downregulation via Ca(2+) release-activated Ca(2+) channels and AMPK activation. Mol Cell Biol 31:3546-56 (2011). WB ; Rat . PubMed: 21730292
- Schilling B et al. Proteomic analysis of succinate dehydrogenase and ubiquinol-cytochrome c reductase (Complex II and III) isolated by immunoprecipitation from bovine and mouse heart mitochondria. Biochim Biophys Acta 1762:213-22 (2006). PubMed: 16120479