Anti-Complex IV Immunocapture antibody [31E91B82G9] (ab109863)
![Immunocytochemistry/ Immunofluorescence - Anti-Complex IV Immunocapture antibody [31E91B82G9] (ab109863)](https://www.abcam.com/ps/products/109/ab109863/Images/ab109863-208840-anti-complex-iv-immunocapture-antibody-31e91b82g9-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Complex IV Immunocapture antibody [31E91B82G9] (ab109863)")
Key features and details
- Mouse monoclonal [31E91B82G9] to Complex IV Immunocapture
- Suitable for: ICC/IF, IP, Flow Cyt
- Reacts with: Cow, Human
- Isotype: IgG1
Overview
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Product name
Anti-Complex IV Immunocapture antibody [31E91B82G9] -
Description
Mouse monoclonal [31E91B82G9] to Complex IV Immunocapture -
Host species
Mouse -
Specificity
100 µg ab109863 can capture at least 10 µg Complex IV (COX) from 1 mg solubilized Bovine heart mitochondria. -
Tested applications
Suitable for: ICC/IF, IP, Flow Cytmore details
Unsuitable for: IHC-P or WB -
Species reactivity
Reacts with: Cow, Human -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- Bovine heart and Human mitochondria IF/ICC: HeLa cell line
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purification notes
ab109863 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
31E91B82G9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
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| IP |
Use at 100-1 µg/mg of lysate.
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| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
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IP
Use at 100-1 µg/mg of lysate. |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
Complex IV, also called cytochrome c oxidoreductase or cytochrome c oxidase, is a complex of 13 different subunits, three of which (I, II and III) are encoded on mitochondrial DNA and the remainder in the nuclear DNA. The complex contains two heme groups (a and a3) and two copper atoms as prosthetic groups. Genetic alterations of this enzyme complex are a common cause of OXPHOS diseases and the enzyme is altered in patients with Alzheimer’s disease. Also, there are reports of reduced amounts of this complex in hypoxic cancer cells. -
Cellular localization
Mitochondrial
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Complex IV Immunocapture antibody [31E91B82G9] (ab109863)
ICC/IF image of ab109863 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109863, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Flow Cytometry - Anti-Complex IV Immunocapture antibody [31E91B82G9] (ab109863)](https://www.abcam.com/ps/products/109/ab109863/Images/ab109863-195285-anti-complex-iv-immunocapture-antibody-31e91b82g9-flow-cytometry.jpg)
Flow Cytometry - Anti-Complex IV Immunocapture antibody [31E91B82G9] (ab109863)Overlay histogram showing HepG2 cells stained with ab109863 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109863, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Immunoprecipitation - Anti-Complex IV Immunocapture antibody [31E91B82G9] (ab109863)](https://www.abcam.com/ps/products/109/ab109863/Images/ab109863-122854-anti-complex-iv-immunocapture-antibody-31e91b82g9-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Complex IV Immunocapture antibody [31E91B82G9] (ab109863)Staining of Complex IV Lane 1: 0.1 mg Human mitochondrial lysate Lane 2: 0.1 mg Bovine mitochondrial lysate Lane 3: 0.025 mg Human mitochondrial lysate Lane 4: 0.025 mg Bovine mitochondrial lysate Immunoprecipitated using ab109863 at 100 µg/mg lysate
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab109863 has been referenced in 4 publications.
- Du F et al. Gain of PITRM1 peptidase in cortical neurons affords protection of mitochondrial and synaptic function in an advanced age mouse model of Alzheimer's disease. Aging Cell 20:e13368 (2021). PubMed: 33951271
- Bogenhagen DF & Haley JD Pulse-chase SILAC-based analyses reveal selective oversynthesis and rapid turnover of mitochondrial protein components of respiratory complexes. J Biol Chem 295:2544-2554 (2020). PubMed: 31974161
- Escobar-Alvarez S et al. Inhibition of human peptide deformylase disrupts mitochondrial function. Mol Cell Biol 30:5099-109 (2010). PubMed: 20805355
- Nadanaciva S et al. Target identification of drug induced mitochondrial toxicity using immunocapture based OXPHOS activity assays. Toxicol In Vitro 21:902-11 (2007). PubMed: 17346924
