Recombinant Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)
![Western blot - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)](https://www.abcam.com/ps/products/235/ab235585/Images/ab235585-386555-anticonnexin-43-gja1-antibody-epr22955101-western-blot-huh7-hela-c2c12.jpg "Western blot - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22955-101] to Connexin 43 / GJA1 - Intercellular Junction Marker
- Suitable for: IP, IHC-Fr, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker
See all Connexin 43 / GJA1 primary antibodies -
Description
Rabbit monoclonal [EPR22955-101] to Connexin 43 / GJA1 - Intercellular Junction Marker -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-Fr, IHC-P, WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse brain, mouse heart, rat brain and human brain lysates. IHC-P: Human, mouse and rat cardiac muscle tissues. Mouse testis tissue. IP: Mouse brain lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22955-101 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/30.
|
|
| IHC-Fr |
1/250.
|
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Use at 1/500 for human. Use at 1/1000 for mouse and rat. |
|
| WB |
1/1000.
|
| Notes |
|---|
|
IP
1/30. |
|
IHC-Fr
1/250. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Use at 1/500 for human. Use at 1/1000 for mouse and rat. |
|
WB
1/1000. |
Target
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Function
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. -
Tissue specificity
Expressed in the heart and fetal cochlea. -
Involvement in disease
Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
Defects in GJA1 are the cause of autosomal recessive oculodentodigital dysplasia (ODDD autosomal recessive) [MIM:257850].
Defects in GJA1 may be the cause of syndactyly type 3 (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.
Defects in GJA1 are a cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
Defects in GJA1 are a cause of Hallermann-Streiff syndrome (HSS) [MIM:234100]. HSS is a disorder characterized by a typical skull shape (brachycephaly with frontal bossing), hypotrichosis, microphthalmia, cataracts, beaked nose, micrognathia, skin atrophy, dental anomalies and proportionate short stature. Mental retardation is present in a minority of cases. -
Sequence similarities
Belongs to the connexin family. Alpha-type (group II) subfamily. -
Cellular localization
Cell membrane. Cell junction > gap junction. - Information by UniProt
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Database links
- Entrez Gene: 2697 Human
- Entrez Gene: 14609 Mouse
- Entrez Gene: 24392 Rat
- Omim: 121014 Human
- SwissProt: P17302 Human
- SwissProt: P23242 Mouse
- SwissProt: P08050 Rat
- Unigene: 74471 Human
see all -
Alternative names
- Connexin 43 antibody
- Connexin-43 antibody
- Cx 43 antibody
see all
Images
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Western blot - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)All lanes : Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585) at 1/1000 dilution
Lane 1 : Huh7( Human hepatocellular carcinoma epithelial cell)whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : C2C12 (Mouse myoblasts myoblast)whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution
Exposure time: 180 secondsab235585 has low affinity in cell line samples.
Blocking and dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)](https://www.abcam.com/ps/products/235/ab235585/Images/ab235585-353430-anti-connexin-43-gja1-epr22955-101-western-blot.jpg)
Western blot - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)All lanes : Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate at 20 µg
Lane 2 : Mouse heart tissue lysate at 20 µg
Lane 3 : Rat brain tissue lysate at 10 µg
Lane 4 : Human brain tissue lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 43 kDa why is the actual band size different from the predicted?Blocking and Dilution Buffer and concentration: 5% NFDM/TBST. The expression profile is consistent with the existence of natural variants described in the literature (PMID: 24210816, 28576298). Exposure times: Lane 1: 59 seconds; Lane 2: 15 seconds; Lane 3: 15 seconds; Lane 4: 26 seconds.
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![Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)](https://www.abcam.com/ps/products/235/ab235585/Images/ab235585-7-anti-connexin-43-gja1-epr22955-101-immunoprecipitation-brain-mouse.jpg)
Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)Connexin 43 / GJA1 was immunoprecipitated from 0.35 mg mouse brain lysate 10µg with ab235585 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab235585 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain lysate 10µg.
Lane 2: ab235585 IP in mouse brain lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab235585 in mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
High MW band between 75kDa and 100kDa represents Connexin 43 dimers which described in literature. (PMID: 19460776).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)](https://www.abcam.com/ps/products/235/ab235585/Images/ab235585-6-anti-connexin-43-gja1-epr22955-101-immunohistochemistry-cardiac-muscle-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Connexin 43 / GJA1 with ab235585 at 1/1000 dilution (0.507 µg/ml) followed by a ready to use Goat Anti-Rabbit & Mouse IgG (HRP). Positive staining on rat myocardial intercalated disc is observed. The section was incubated with ab235585 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit & Mouse IgG (HRP).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)](https://www.abcam.com/ps/products/235/ab235585/Images/ab235585-5-anti-connexin-43-gja1-epr22955-101-immunohistochemistry-testis-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Connexin 43 / GJA1 with ab235585 at 1/1000 dilution (0.507 µg/ml) followed by a ready to use Goat Anti-Rabbit & Mouse IgG (HRP). Membranous staining on mouse testis is observed. The section was incubated with ab235585 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit & Mouse IgG (HRP).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)](https://www.abcam.com/ps/products/235/ab235585/Images/ab235585-4-anti-connexin-43-gja1-epr22955-101-immunohistochemistry-cardiac-muscle-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Connexin 43 / GJA1 with ab235585 at 1/1000 dilution (0.507 µg/ml) followed by a ready to use Goat Anti-Rabbit & Mouse IgG (HRP). Positive staining on mouse myocardial intercalated disc (PMID: 30228305) is observed. The section was incubated with ab235585 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit & Mouse IgG (HRP).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)](https://www.abcam.com/ps/products/235/ab235585/Images/ab235585-3-anti-connexin-43-gja1-epr22955-101-immunohistochemistry-cardiac-muscle-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Connexin 43 / GJA1 with ab235585 at 1/500 dilution (1.01 µg/ml) followed by a ready to use Goat Anti-Rabbit & Mouse IgG (HRP). Positive staining on human myocardial intercalated disc is observed. The section was incubated with ab235585 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit & Mouse IgG (HRP).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Immunohistochemistry (Frozen sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)](https://www.abcam.com/ps/products/235/ab235585/Images/ab235585-2-anti-connexin-43-gja1-epr22955-101-ihcfr-cardiac-muscle-rat.jpg)
Immunohistochemistry (Frozen sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen section of rat cardiac muscle tissue labeling Connexin 43 / GJA1 using ab235585 at 1/250 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). The nuclear counterstain is DAPI (blue). Positive staining on intercalated discs of rat cardiac muscle (PMID: 18678643) is observed.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Immunohistochemistry (Frozen sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)](https://www.abcam.com/ps/products/235/ab235585/Images/ab235585-1-anti-connexin-43-gja1-epr22955-101-ihcfr-cardiac-muscle-mouse.jpg)
Immunohistochemistry (Frozen sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen section of mouse cardiac muscle tissue labeling Connexin 43 / GJA1 using ab235585 at 1/250 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). The nuclear counterstain is DAPI (blue). Positive staining on intercalated discs of mouse cardiac muscle (PMID: 18678643) is observed.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)](https://www.abcam.com/ps/products/235/ab235585/Images/ab235585-11-benefits-of-recombinant-antibodies.png)
Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (ab235585)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (8)
ab235585 has been referenced in 8 publications.
- Zhang W et al. Tbx18 promoted the conversion of human-induced pluripotent stem cell-derived cardiomyocytes into sinoatrial node-like pacemaker cells. Cell Biol Int 46:403-414 (2022). PubMed: 34882885
- de Leeuw SM et al. APOE2, E3, and E4 differentially modulate cellular homeostasis, cholesterol metabolism, and inflammatory response in isogenic iPSC-derived astrocytes. Stem Cell Reports 17:110-126 (2022). PubMed: 34919811
- Zhang XY et al. Sishen Pill Maintained Colonic Mucosal Barrier Integrity to Treat Ulcerative Colitis via Rho/ROCK Signaling Pathway. Evid Based Complement Alternat Med 2021:5536679 (2021). PubMed: 34925530
- Zhang T et al. Identification of key transcriptome biomarkers based on a vital gene module associated with pathological changes in Alzheimer's disease. Aging (Albany NY) 13:14940-14967 (2021). PubMed: 34031265
- Lim WW et al. Electrophysiological and Structural Remodeling of the Atria in a Mouse Model of Troponin-I Mutation Linked Hypertrophic Cardiomyopathy: Implications for Atrial Fibrillation. Int J Mol Sci 22:N/A (2021). PubMed: 34203369
- Chen Z et al. Hydrogen Sulfide Contributes to Uterine Quiescence Through Inhibition of NLRP3 Inflammasome Activation by Suppressing the TLR4/NF-κB Signalling Pathway. J Inflamm Res 14:2753-2768 (2021). PubMed: 34234503
- Zeng W et al. MicroRNA-206 relieves irradiation-induced neuroinflammation by regulating connexin 43. Exp Ther Med 22:1186 (2021). PubMed: 34475976
- Yu H et al. Targeting connexin 43 provides anti-inflammatory effects after intracerebral hemorrhage injury by regulating YAP signaling. J Neuroinflammation 17:322 (2020). PubMed: 33115476