Anti-Constitutive androstane receptor antibody (ab186869)
Key features and details
- Rabbit polyclonal to Constitutive androstane receptor
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Constitutive androstane receptor antibody
See all Constitutive androstane receptor primary antibodies -
Description
Rabbit polyclonal to Constitutive androstane receptor -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Constitutive androstane receptor aa 103-352 (C terminal).
Database link: Q14994 -
Positive control
- U87 and BT474 cell lysates; Mouse liver tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
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| WB | (2) |
1/500 - 1/2000. Predicted molecular weight: 40 kDa.
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| IHC-P |
1/50 - 1/200.
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| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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WB
1/500 - 1/2000. Predicted molecular weight: 40 kDa. |
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IHC-P
1/50 - 1/200. |
Target
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Function
Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element. -
Tissue specificity
Predominantly expressed in liver. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed by a short N-terminal domain followed by the DNA binding, hinge, and ligand binding/dimerization domains. -
Post-translational
modificationsPhosphorylated at Thr-38 by PKC, dephosphorylation of Thr-38 is required for nuclear translocation and activation. -
Cellular localization
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton. Recruited to the cytoplasm by DNAJC7. - Information by UniProt
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Database links
- Entrez Gene: 9970 Human
- Entrez Gene: 12355 Mouse
- Entrez Gene: 65035 Rat
- Omim: 603881 Human
- SwissProt: Q14994 Human
- SwissProt: O35627 Mouse
- SwissProt: Q9QUS1 Rat
- Unigene: 349642 Human
see all -
Alternative names
- CAR antibody
- CAR BETA antibody
- CAR1 antibody
see all
Images
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Western blot - Anti-Constitutive androstane receptor antibody (ab186869)All lanes : Anti-Constitutive androstane receptor antibody (ab186869) at 1/500 dilution
Lane 1 : U87 cell lysate
Lane 2 : BT474 cell lysate
Lane 3 : Mouse liver tissue lysate
Predicted band size: 40 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Constitutive androstane receptor antibody (ab186869)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling Constitutive androstane receptor with ab186869 at 1/100. Magnification: 400x. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Constitutive androstane receptor antibody (ab186869)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophagus tissue labelling Constitutive androstane receptor with ab186869 at 1/100. Magnification: 400x. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Constitutive androstane receptor antibody (ab186869)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophageal cancer tissue labelling Constitutive androstane receptor with ab186869 at 1/100. Magnification: 400x. -
Immunocytochemistry/ Immunofluorescence - Anti-Constitutive androstane receptor antibody (ab186869)Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186869. Blue DAPI for nuclear staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab186869 has been referenced in 4 publications.
- Deng Y et al. Long non-coding RNA F11-AS1 inhibits HBV-related hepatocellular carcinoma progression by regulating NR1I3 via binding to microRNA-211-5p. J Cell Mol Med 24:1848-1865 (2020). PubMed: 31880390
- Kovács P et al. Lithocholic Acid, a Metabolite of the Microbiome, Increases Oxidative Stress in Breast Cancer. Cancers (Basel) 11:N/A (2019). PubMed: 31461945
- Shubbar MH & Penny JI Effect of amyloid beta on ATP-binding cassette transporter expression and activity in porcine brain microvascular endothelial cells. Biochim Biophys Acta 1862:2314-2322 (2018). WB . PubMed: 30048740
- Wang X et al. Constitutive androstane receptor activation promotes bilirubin clearance in a murine model of alcoholic liver disease. Mol Med Rep 15:3459-3466 (2017). PubMed: 28393244