Anti-COPB2 antibody (ab2915)
Key features and details
- Rabbit polyclonal to COPB2
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
-
Product name
Anti-COPB2 antibody
See all COPB2 primary antibodies -
Description
Rabbit polyclonal to COPB2 -
Host species
Rabbit -
Specificity
Detects coatomer-protein I beta (COP I beta). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Cow, Cynomolgus monkey
-
Immunogen
Synthetic peptide corresponding to Rat COPB2 aa 494-511.
Sequence:KVLAAQETHEGVTEDGIE
(Peptide available asab4933) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COP II facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 2 µg/ml. Predicted molecular weight: 115 kDa.
|
| Notes |
|---|
|
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 115 kDa. |
Target
-
Function
The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
This coatomer complex protein, essential for Golgi budding and vesicular trafficking, is a selective binding protein (RACK) for protein kinase C, epsilon type. It binds to Golgi membranes in a GTP-dependent manner. -
Sequence similarities
Belongs to the WD repeat COPB2 family.
Contains 9 WD repeats. -
Cellular localization
Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. - Information by UniProt
-
Database links
- Entrez Gene: 281706 Cow
- Entrez Gene: 50797 Mouse
- Entrez Gene: 60384 Rat
- SwissProt: P35605 Cow
- SwissProt: Q4R4I8 Cynomolgus monkey
- SwissProt: O55029 Mouse
- SwissProt: O35142 Rat
- Unigene: 400464 Mouse
see all -
Alternative names
- Beta'-coat protein antibody
- Beta'-COP antibody
- Coatomer subunit beta' antibody
see all
Images
-
Western blot - Anti-COPB2 antibody (ab2915)Western blot detection of beta COP I from PC-12 cell lysates using ab2915.
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab2915 has been referenced in 2 publications.
- Li X et al. Hyperresponsiveness of human gingival fibroblasts from patients with aggressive periodontitis against bacterial lipopolysaccharide. Exp Ther Med 21:417 (2021). PubMed: 33747158
- Šimic Bilandžija T et al. Spatio-Temporal Expression Pattern of Ki-67, pRB, MMP-9 and Bax in Human Secondary Palate Development. Life (Basel) 11:N/A (2021). PubMed: 33672637