Recombinant Anti-Coronin-1C antibody [EPR25365-24] KO Tested (ab283693)

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Western blot - Anti-Coronin-1C antibody [EPR25365-24] (ab283693)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR25365-24] to Coronin-1C
Suitable for: WB, IHC-P, IP
Knockout validated
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-Coronin-1C antibody [EPR25365-24]
See all Coronin-1C primary antibodies
Description

Rabbit monoclonal [EPR25365-24] to Coronin-1C
Host species

Rabbit
Specificity

IHC and IP suitable for human only.
Tested applications

Suitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt (Intra) or ICC/IF
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HepG2, A549, Neuro-2a, HL-1, C6, HeLa, Human heart, His-tagged human Coronin-1C recombinant protein, Wild-type HEK-293T. IHC-P: Human breast, breast cancer and lymphoid nodule tissue. IP: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR25365-24
Isotype

IgG
Research areas

Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- VeriBlot for IP Detection Reagent (HRP) (ab131366)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab283693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P		1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC suitable for human only.
IP		1/30. IP suitable for human only.

Notes
WB 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC suitable for human only.
IP 1/30. IP suitable for human only.

Application notes

Is unsuitable for Flow Cyt (Intra) or ICC/IF.

Function

May be involved in cytokinesis, motility, and signal transduction.
Tissue specificity

Ubiquitous.
Sequence similarities

Belongs to the WD repeat coronin family.
Contains 4 WD repeats.
Target information above from: UniProt accession Q9ULV4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 23603 Human
- Entrez Gene: 23790 Mouse
- Entrez Gene: 501841 Rat
- Omim: 605269 Human
- SwissProt: Q9ULV4 Human
- SwissProt: Q9WUM4 Mouse
- Unigene: 330384 Human
- Unigene: 260158 Mouse
Alternative names
- COR1C_HUMAN antibody
- CORO 1C antibody
- Coro1c antibody
- Coronin 1c antibody
- Coronin 3 antibody
- Coronin actin binding protein 1C antibody
- Coronin-1C antibody
- Coronin-3 antibody
- Coronin1c antibody
- Coronin3 antibody
- CRNN 4 antibody
- CRNN4 antibody
- HCRNN 4 antibody
- hCRNN4 antibody
see all

Western blot - Anti-Coronin-1C antibody [EPR25365-24] (ab283693)

All lanes : Anti-Coronin-1C antibody [EPR25365-24] (ab283693) at 1/1000 dilution

Lane 1 : Wild-type 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : Coronin-1C knockout HEK-293T whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at 1/20000 dilution

Predicted band size: 53 kDa
Observed band size: 53 kDa

Blocking buffer and concentration: ½ volume of Odyssey Blocking Buffer (TBS)+ ½ volume of 0.1% TBS Diluting buffer and concentration: ½ volume of Odyssey Blocking Buffer (TBS)+ ½ volume of 0.1% TBST

False colour image of Western blot: Anti-Coronin-1C antibody [EPR25365-24] (ab283693) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red.

In Western blot, ab283693 was shown to bind specifically to Coronin-1C. A band was observed at 53 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in Coronin-1C knockout cell line ab266381 (knockout cell lysate (ab258377).

To generate this image, wild-type and Coronin-1C knockout HEK-293T cell lysates were analyzed. First, samples were run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in Intercept^® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
Western blot - Anti-Coronin-1C antibody [EPR25365-24] (ab283693)

All lanes : Anti-Coronin-1C antibody [EPR25365-24] (ab283693) at 1/1000 dilution

Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 2 : A549 (human lung carcinoma epithelial cell), whole cell lysate
Lane 3 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
Lane 4 : HL-1 (mouse atrial muscle cell), whole cell lysate
Lane 5 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 6 : Human heart tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 53 kDa
Observed band size: 53 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 3 minutes
Western blot - Anti-Coronin-1C antibody [EPR25365-24] (ab283693)

All lanes : Anti-Coronin-1C antibody [EPR25365-24] (ab283693)

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : Hela transfected with CORO1C siRNA 1 whole cell lysate
Lane 3 : Hela transfected with CORO1C siRNA 2 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 53 kDa
Observed band size: 53 kDa

Blocking and diluting buffer concentration: 5% NFDM/TBST

Exposure time 26 seconds
Western blot - Anti-Coronin-1C antibody [EPR25365-24] (ab283693)

All lanes : Anti-Coronin-1C antibody [EPR25365-24] (ab283693) at 1/1000 dilution

Lane 1 : His-tagged human Coronin-1C recombinant protein
Lane 2 : His-tagged human Coronin-1B recombinant protein
Lane 3 : His-tagged human Coronin-6 recombinant protein
Lane 4 : His-tagged human Coronin-1A recombinant protein

Lysates/proteins at 0.005 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 53 kDa
Observed band size: 53 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST

This antibody has no cross-reaction with human Coronin-1B, Coronin-6 and Coronin-1A.

All the recombinant proteins were made in-house.

All the samples are E.coli extracts containing Coronin-1C, Coronin-1B, Coronin-6 or Coronin-1A recombinant protein respectively.

Exposure time: 26 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin-1C antibody [EPR25365-24] (ab283693)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labelling Coronin-1C with ab283693 at 1/2000 (0.301 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human breast (PMID: 30065298). The section was incubated with ab283693 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin-1C antibody [EPR25365-24] (ab283693)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labelling Coronin-1C with ab283693 at 1/2000 (0.301 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human breast cancer (PMID: 30065298). The section was incubated with ab283693 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin-1C antibody [EPR25365-24] (ab283693)

Immunohistochemical analysis of paraffin-embedded Human lymphoid nodule tissue labelling Coronin-1C with ab283693 at 1/2000 (0.301 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human lymphoid nodule (PMID: 20690162). The section was incubated with ab283693 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunoprecipitation - Anti-Coronin-1C antibody [EPR25365-24] (ab283693)

Coronin-1C was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 ug with ab283693 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283693 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 ug

Lane 2: ab283693 IP in HeLa whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab283693 in HeLa whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes
Anti-Coronin-1C antibody [EPR25365-24] (ab283693)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

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Publishing research using ab283693? Please let us know so that we can cite the reference in this datasheet.

ab283693 has not yet been referenced specifically in any publications.

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Compatible Secondaries

Isotype control

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (0)

Customer reviews and Q&As