Anti-COX IV antibody [20E8C12] (ab14744)
Key features and details
- Mouse monoclonal [20E8C12] to COX IV
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Anti-COX IV antibody [20E8C12]
See all COX IV primary antibodies -
Description
Mouse monoclonal [20E8C12] to COX IV -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human
Predicted to work with: Pig -
Immunogen
Tissue, cells or virus corresponding to Cow COX IV.
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Positive control
- Human, bovine, murine and rat heart mitochondria.
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General notes
This antibody makes an effective loading control for mitochondria. COXIV is generally expressed at a consistent high level. However, be aware that many proteins run at the same 16kD size as COXIV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for proteins of this size. Some caution is required when using this antibody as a loading control as COXIV expression can vary under some manipulations. An alternative mitochondrial loading control is Rabbit polyclonal to COX IV antibody (ab16056).
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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Purity
IgG fraction -
Primary antibody notes
This antibody makes an effective loading control for mitochondria. COXIV is generally expressed at a consistent high level. However, be aware that many proteins run at the same 16kD size as COXIV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for proteins of this size. Some caution is required when using this antibody as a loading control as COXIV expression can vary under some manipulations. An alternative mitochondrial loading control is Rabbit polyclonal to COX IV antibody (ab16056). -
Clonality
Monoclonal -
Clone number
20E8C12 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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WB | (18) |
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa.
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Notes |
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Flow Cyt
Use 0.5µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa. |
Target
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Function
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the cytochrome c oxidase IV family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 1327 Human
- Entrez Gene: 12857 Mouse
- Entrez Gene: 29445 Rat
- Omim: 123864 Human
- SwissProt: P00423 Cow
- SwissProt: P13073 Human
- SwissProt: P19783 Mouse
- SwissProt: P10888 Rat
see all -
Alternative names
- AL024441 antibody
- COX 4 antibody
- COX IV 1 antibody
see all
Images
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All lanes : Anti-COX IV antibody [20E8C12] (ab14744) at 1/5000 dilution
Lane 1 : Cytoplasmic fraction mouse NIH/3T3 cell lysate
Lane 2 : Nuclear fraction mouse NIH/3T3 cell lysate
Secondary
All lanes : HRP conjugated goat anti-mouse antibody
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 16 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
This image is courtesy of an Abreview submitted by Camilla Skjerpen on 4 July 2005. -
Overlay histogram showing HeLa cells stained with ab14744 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14744, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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All lanes : Anti-COX IV antibody [20E8C12] (ab14744) at 1 µg/ml
Lane 1 : Isolated mitochondria from human heart at 5 µg
Lane 2 : Isolated mitochondria from bovine heart at 1 µg
Lane 3 : Isolated mitochondria from rat heart at 10 µg
Lane 4 : Isolated mitochondria from murine heart at 10 µg
Observed band size: 16 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (269)
ab14744 has been referenced in 269 publications.
- Aleo SJ et al. Drug repositioning as a therapeutic strategy for neurodegenerations associated with OPA1 mutations. Hum Mol Genet 29:3631-3645 (2021). PubMed: 33231680
- Shannon CE et al. Insulin resistance is mechanistically linked to hepatic mitochondrial remodeling in non-alcoholic fatty liver disease. Mol Metab 45:101154 (2021). PubMed: 33359401
- Esparza-Moltó PB et al. Generation of mitochondrial reactive oxygen species is controlled by ATPase inhibitory factor 1 and regulates cognition. PLoS Biol 19:e3001252 (2021). PubMed: 33983919
- Pan Y et al. Mitochondrial proteomics alterations in rat hearts following ischemia/reperfusion and diazoxide post-conditioning. Mol Med Rep 23:N/A (2021). PubMed: 33355377
- Yang K et al. Dopamine receptor D1 agonist inhibits glioblastoma via calpain-mediated ER stress and mitochondrial dysfunction. Oncol Rep 45:N/A (2021). PubMed: 33760205