Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)
Key features and details
- Mouse monoclonal [mAbcam33985] to COX IV - Mitochondrial Marker
- Suitable for: WB, Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse, Cow, Human, Xenopus laevis
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker
See all COX IV primary antibodies -
Description
Mouse monoclonal [mAbcam33985] to COX IV - Mitochondrial Marker -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt (Intra), ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Cow, Human, Xenopus laevis
Predicted to work with: Rat, Sheep, Chimpanzee, Monkey, Zebrafish, African green monkey, Chinese hamster, Drosophila C virus -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat and HepG2 whole cell lysates and human skeletal muscle, mouse skeletal muscle and cow kidney tissue lysates. ICC/IF: HeLa cells; Flow Cyt (Intra): HeLa cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
mAbcam33985 -
Myeloma
Sp2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33985 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (13) |
Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
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Flow Cyt (Intra) |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF | (9) |
Use a concentration of 1 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa). |
Flow Cyt (Intra)
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the cytochrome c oxidase IV family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 454391 Chimpanzee
- Entrez Gene: 1327 Human
- Entrez Gene: 12857 Mouse
- Entrez Gene: 29445 Rat
- Entrez Gene: 326975 Zebrafish
- Omim: 123864 Human
- SwissProt: O46577 Chimpanzee
- SwissProt: P00423 Cow
see all -
Alternative names
- AL024441 antibody
- COX 4 antibody
- COX IV 1 antibody
see all
Images
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All lanes : Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 4 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 5 : Kidney (Cow) Tissue Lysate (ab29073)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 1 minute -
Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)
Ab33985 staining COX IV in HeLa (Human cervix adenocarcinoma epithelial cell) cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1:1000 dilution (1µg/ml). An Alexa Fluor®594 Goat anti-mouse (ab150120) was used as a secondary antibody at 1:1000 dilution (2 µg/ml). Cells were counterstained with anti-Cyclophilin F (ab231155, 5.5µg/ml) and AlexaFluor®488 Goat anti-Rabbit (ab150077, 2µg/ml). DAPI was used as a nuclear counterstain. Ab33985 was used for negative control 1 at 1:1000 dilution (1µg/ml). For negative control 2, ab231155 was used at a 1:100 dilution (5.5µg/ml) and ab150129 was used as a secondary antibody at 1:1000 dilution (2µg/ml). Confocal image showing mitochondrial staining in HeLa cell line.
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Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)
ICC/IF image of ab33985 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33985, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Flow Cytometry (Intracellular) - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)
Overlay histogram showing HeLa cells stained with ab33985 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33985, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)This image is courtesy of an Abreview submitted by Dr Anne-Lore SchlaitzAll lanes : Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) at 1/1000 dilution
Lane 1 : Crude extract prepared from Xenopus laevis egg
Lane 2 : Cytosol lysate prepared from Xenopus laevis egg extract
Lane 3 : Total membrane lysate prepared from Xenopus laevis egg extract
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP conjugated donkey anti-mouse IgG at 1/4000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 90 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)This image is courtesy of an anonymous Abreview
ab33985 staining COX IV in human proximal tubular epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 in PBS and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS) for 45 minutes at 20°C. ab6785, a FITC-conjugated goat anti-mouse IgG (H+L) polyclonal was used as the secondary antibody (1/1000).
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Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)This image is courtesy of an anonymous AbreviewAll lanes : Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) at 1/1000 dilution
All lanes : cow liver membrane with Milk
Lysates/proteins at 15 µg per lane.
Blocking peptides at 1.5 % per lane.
Secondary
All lanes : Goat Anti-mouse IgG HRP at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Exposure time: 17 hours
Datasheets and documents
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SDS download
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Datasheet download
References (109)
ab33985 has been referenced in 109 publications.
- Flory S et al. Uptake and time-dependent subcellular localization of native and micellar curcumin in intestinal cells. Biofactors 48:897-907 (2022). PubMed: 35170815
- Sonn SK et al. Peroxiredoxin 3 deficiency induces cardiac hypertrophy and dysfunction by impaired mitochondrial quality control. Redox Biol 51:102275 (2022). PubMed: 35248828
- Shi J et al. Targeting glutamine utilization to block metabolic adaptation of tumor cells under the stress of carboxyamidotriazole-induced nutrients unavailability. Acta Pharm Sin B 12:759-773 (2022). PubMed: 35256945
- Barreto BR et al. Cocaine Modulates the Neuronal Endosomal System and Extracellular Vesicles in a Sex-Dependent Manner. Neurochem Res 47:2263-2277 (2022). PubMed: 35501523
- Spurgeon ME et al. Merkel cell polyomavirus large T antigen binding to pRb promotes skin hyperplasia and tumor development. PLoS Pathog 18:e1010551 (2022). PubMed: 35560034