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    products/primary-antibodies/cox1--cyclooxygenase-1-antibody-epr5866-ab109025.pdf

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RecombinantRabMAb

Recombinant Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

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Reviews (2)Q&A (3)References (27)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
  • Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
  • Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
  • Immunoprecipitation - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
  • Flow Cytometry (Intracellular) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
  • Immunocytochemistry/ Immunofluorescence - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
  • Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
  • Immunocytochemistry/ Immunofluorescence - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
  • Flow Cytometry (Intracellular) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
  • OI-RD Scanning - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
  • Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5866] to COX1 / Cyclooxygenase 1
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free HRP PE

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Recombinant human COX1 / Cyclooxygenase 1 protein (ab198643)
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Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)

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Overview

  • Product name

    Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866]
    See all COX1 / Cyclooxygenase 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5866] to COX1 / Cyclooxygenase 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NIH/3T3, HaCaT, Neuro -2a, C2C12, A431, and L6 cell lysates. IHC-P: Human skin, human cerebrum, mouse kidney, and rat kidney tissues. ICC/IF: HeLa and Neuro-2a cells. Flow Cyt (intra): NIH/3T3 cells. IP: C2C12 cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 5.50 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5866
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Blood
    • Platelets
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Prostaglandins
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199027)
    • Alexa Fluor® 647 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199202)
    • HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199203)
    • PE Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab209581)
    • Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] - BSA and Azide free (ab219375)
  • Assay kits

    • Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (ab204699)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • A-431 membrane extract lysate (ab30134)
  • Recombinant Protein

    • Recombinant human COX1 / Cyclooxygenase 1 protein (ab198643)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (1)
1/1000 - 1/10000. Predicted molecular weight: 69 kDa.
IP
1/10 - 1/100.
IHC-P (1)
1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Heat up to 98 °C, below boiling, and then let cool for 10-20 min.

For unpurified use at 1/250 - 1/500.

ICC/IF
Use at an assay dependent concentration.
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Predicted molecular weight: 69 kDa.
IP
1/10 - 1/100.
IHC-P
1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Heat up to 98 °C, below boiling, and then let cool for 10-20 min.

For unpurified use at 1/250 - 1/500.

ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
  • Pathway

    Lipid metabolism; prostaglandin biosynthesis.
  • Sequence similarities

    Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • Cellular localization

    Microsome membrane. Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession P23219 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5742 Human
    • Entrez Gene: 19224 Mouse
    • Entrez Gene: 24693 Rat
    • Omim: 176805 Human
    • SwissProt: P23219 Human
    • SwissProt: P22437 Mouse
    • SwissProt: Q63921 Rat
    • Unigene: 201978 Human
    • Unigene: 275434 Mouse
    • Unigene: 44404 Rat
    see all
  • Alternative names

    • COX 1 antibody
    • COX 3 antibody
    • COX-1 antibody
    • COX1 antibody
    • Cox3 antibody
    • Cyclooxygenase 1 antibody
    • Cyclooxygenase 3, included antibody
    • Cyclooxygenase-1 antibody
    • EC 1.14.99.1 antibody
    • Partial COX1 proteins, included antibody
    • PCOX1 antibody
    • PGG/HS antibody
    • PGH synthase 1 antibody
    • PGH1_HUMAN antibody
    • PGHS 1 antibody
    • PGHS-1 antibody
    • PGHS1 antibody
    • PHS 1 antibody
    • PHS1 antibody
    • Prostaglandin endoperoxide synthase 1 antibody
    • Prostaglandin G/H synthase 1 antibody
    • Prostaglandin H2 synthase 1 antibody
    • Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 1 antibody
    • PTGHS antibody
    • PTGS1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling COX1 / Cyclooxygenase 1 with Purified ab109025 at 1:150 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution.

    PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
    Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
    All lanes : Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) at 1/10000 dilution (purified)

    Lane 1 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates
    Lane 2 : L6 (Rat skeletal muscle myoblast) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 69 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
    Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
    All lanes : Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) at 1/2000 dilution (purified)

    Lane 1 : HaCaT (Human skin keratinocyte) whole cell lysates
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 69 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
    Immunoprecipitation - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    ab109025 (purified) at 1:20 dilution (0.8μg) immunoprecipitating COX1 / Cyclooxygenase 1 in C2C12 whole cell lysate.

    Lane 1 (input): C2C12 (Mouse myoblasts myoblast) whole cell lysate,10μg
    Lane 2 (+): ab109025 & C2C12 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109025 in C2C12 whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

  • Flow Cytometry (Intracellular) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
    Flow Cytometry (Intracellular) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    Intracellular Flow Cytometry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling COX1 / Cyclooxygenase 1 with purified ab109025 at 1/100 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling COX1 / Cyclooxygenase 1 with Purified ab109025 at 1:150 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution.

    PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling COX1 / Cyclooxygenase 1 with Purified ab109025 at 1:150 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution.

    PBS instead of the primary antibody was used as the negative control.

  • Immunocytochemistry/ Immunofluorescence - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
    Immunocytochemistry/ Immunofluorescence - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    Immunocytochemistry/Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma) labelling COX1 with purified ab109025 at 1/50. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only.

  • Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
    Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
    All lanes : Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) at 1/1000 dilution (unpurified)

    Lane 1 : NIH/3T3 cell lysate
    Lane 2 : HaCaT cell lysate
    Lane 3 : Neuro 2a cell lysate
    Lane 4 : C2C12 cell lysate
    Lane 5 : A431 cell lysate
    Lane 6 : L6 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 69 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    Unpurified ab109025 at 1/250 dilution staining COX1 / Cyclooxygenase 1 in human skin by immunohistochemistry, paraffin-embedded tissue.

  • Immunocytochemistry/ Immunofluorescence - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
    Immunocytochemistry/ Immunofluorescence - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    Unpurified ab109025 at 1/100 dilution staining COX1 / Cyclooxygenase 1 in HeLa cells by Immunofluorescence.

  • Flow Cytometry (Intracellular) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
    Flow Cytometry (Intracellular) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

    Overlay histogram showing NIH/3T3 cells stained with unpurified ab109025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109025, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. 

     

     

  • OI-RD Scanning - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
    OI-RD Scanning - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
    Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (27)

Publishing research using ab109025? Please let us know so that we can cite the reference in this datasheet.

ab109025 has been referenced in 27 publications.

  • Li M  et al. The Arachidonic Acid Metabolism Mechanism Based on UPLC-MS/MS Metabolomics in Recurrent Spontaneous Abortion Rats. Front Endocrinol (Lausanne) 12:652807 (2021). PubMed: 33868179
  • Li Z  et al. Disease Status-Dependent Drug-Herb Interactions: NASH Lowered the Risk of Hepatotoxicity in Rats Coadministered With Simvastatin and Gardenia jasminoides J. Ellis. Front Pharmacol 12:622040 (2021). PubMed: 33967756
  • Yao NA  et al. Preventive Beneficial Effect of an Aqueous Extract of Phyllanthus amarus Schum. and Thonn. (Euphorbiaceae) on DOCA-Salt-Induced Hypertension, Cardiac Hypertrophy and Dysfunction, and Endothelial Dysfunction in Rats. J Cardiovasc Pharmacol 75:573-583 (2020). PubMed: 32187164
  • Piazza VG  et al. Exposure to growth hormone is associated with hepatic up-regulation of cPLA2a and COX. Mol Cell Endocrinol 509:110802 (2020). PubMed: 32259636
  • Farooq MA  et al. Intake of omega-3 formulation EPA:DHA 6:1 by old rats for 2 weeks improved endothelium-dependent relaxations and normalized the expression level of ACE/AT1R/NADPH oxidase and the formation of ROS in the mesenteric artery. Biochem Pharmacol 173:113749 (2020). PubMed: 31830469
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-5 of 5 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
No
Specification
Placenta
Blocking step
Vector Laboratories Supplier Diversity Partner VECTASTAIN Elite ABC HRP Kit as blocking agent for 30 minute(s) · Concentration: 10µg/mL
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Sudeshna Tripathy

Verified customer

Submitted Sep 26 2022

Western blot abreview for Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866]

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Dog Cell lysate - whole cell (dermal fibroblasts)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Treatment
1 μM bradykinin
Specification
dermal fibroblasts
Blocking step
Blockace as blocking agent for 50 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 08 2022

Question

Hi,

I have recently purchased the ab109025 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] and would like to the know any suggestion for the use of high temperature mediated antigen retrieval solution – citrate (pH 6) or high pH (pH 9) suitable for formalin fixed paraffin embedded sections?

Also, is there any problem of condensation involved if I want to aliquot the antibody which is in glycerol?

Best Regards,

Read More

Abcam community

Verified customer

Asked on May 08 2012

Answer

Thank you for your inquiry.

I am happy toconfirm that the image on the datasheet was produced using Citrate buffer pH6 for antigen retrieval. Therefore I suggest to also use this buffer in your experiments with high temperature.

The exact protocol is not available and has to be optimized according to your experimental set-up and samples.

Since this antibody is stored in glycerol and should not freeze through, the only advantage of aliquoting is the contamination prevention. It is therefore not necessary to have very small aliquots and condensation or evaporation should not make problems in this case.

I hope this information is helpful and wish you good luck with your research.

Read More

Abcam Scientific Support

Answered on May 08 2012

Question

phone call: searching antibodies against COX1 and COX2 for IHC-Fr on rat and mouse kidney sections.

Read More

Abcam community

Verified customer

Asked on May 03 2012

Answer

x

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab109025 inIHC auf gefrorenen Schnitten (IHC-Fr) zu testen, annehmen möchten. Entschuldigen Sie bitte, dass ich es nicht mehr geschafft habe, Ihnen den Code gestern zuzusenden.
Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer x, deckt den Wert von ab109025 ab und kann zum Kauf weiterer Produkte eingesetzt werden.

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Answered on May 03 2012

Question

I am looking for a good COX-1 antibody that will specifically pick up a truncated form of COX-1 that I have generated. I found yourab109025 COX-1 monoclonal antibody which appears to give a strong signal, but I am wondering if you have any more information on the epitope that it recognizes. I realize that this may be proprietary information, but I am hoping that you could tell me whether it would detect a COX-1 mutant that is missing the first ˜215 amino acids. If this antibody does not, do you have any recommendations for an antibody that would detect this truncated form?

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Asked on Feb 27 2012

Answer

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The antibody ab109025 would not recognize the protein that is missing the first 250 amino acids. The peptide maps between amino acids 120 and 145 of human Prostaglandin G/H synthase 1/COX1 (UniProt: P23219).



I would recommend ab2338 as we know that this antibody is raised to the C-terminal end.



Please let me know if there is anything else I can help you with.

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Abcam Scientific Support

Answered on Feb 27 2012

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