Recombinant Anti-COX2 / Cyclooxygenase 2 antibody [33/Cox-2] (ab300668)
![Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [33/Cox-2] (ab300668)](https://www.abcam.com/ps/products/300/ab300668/Images/ab300668-3-anti-cox2cyclooxygenase-2-antibody-33cox-2-western-blot-untreated-raw2647-mouse-abelson-murine-leukemia-virus-induced-tumor-macrophage-whole-cell-lysate-20-ug.jpg "Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [33/Cox-2] (ab300668)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [33/Cox-2] to COX2 / Cyclooxygenase 2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-COX2 / Cyclooxygenase 2 antibody [33/Cox-2]
See all COX2 / Cyclooxygenase 2 primary antibodies -
Description
Mouse monoclonal [33/Cox-2] to COX2 / Cyclooxygenase 2 -
Host species
Mouse -
Specificity
Not suitable for human ICC/IF samples.
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Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: LPS treated RAW264.7 whole cell lysate. U-87 MG whole cell lysate. Rat kidney and spleen tissue lysate. ICC/IF: LPS treated RAW264.7 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
33/Cox-2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 69 kDa.
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| ICC/IF |
1/50.
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 69 kDa. |
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ICC/IF
1/50. |
Target
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Function
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
Pathway
Lipid metabolism; prostaglandin biosynthesis. -
Sequence similarities
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
Post-translational
modificationsS-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 5743 Human
- Entrez Gene: 19225 Mouse
- Entrez Gene: 29527 Rat
- Omim: 600262 Human
- SwissProt: P35354 Human
- SwissProt: Q05769 Mouse
- SwissProt: P35355 Rat
- Unigene: 196384 Human
see all -
Alternative names
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
Images
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Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [33/Cox-2] (ab300668)All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [33/Cox-2] (ab300668) at 1/1000 dilution
Lane 1 : Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2 : RAW264.7 treated with 1 µg/ml Lipopolysaccharides (LPS) for 6 hours, whole cell lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 5 : HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution
Observed band size: 69 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative controls: MCF7 (PMID: 24325753, PMID: 16997132), HCT116 (PMID: 14739610).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24260490 ).
The blot of lane 3-4 was developed using a high sensitivity ECL substrate.
Exposure time: Lane 1, 2: 59 seconds; Lane 3-5: 125 seconds.
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![Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [33/Cox-2] (ab300668)](https://www.abcam.com/ps/products/300/ab300668/Images/ab300668-2-anti-cox2cyclooxygenase-2-antibody-33cox-2-western-blot-rat-kidney-tissue-lysate20-ug.jpg)
Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [33/Cox-2] (ab300668)All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [33/Cox-2] (ab300668) at 1/1000 dilution
Lane 1 : Rat kidney tissue lysate
Lane 2 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution
Observed band size: 69 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The blot was developed using a high sensitivity ECL substrate.
Exposure time: 3 minutes.
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![Immunocytochemistry/ Immunofluorescence - Anti-COX2 / Cyclooxygenase 2 antibody [33/Cox-2] (ab300668)](https://www.abcam.com/ps/products/300/ab300668/Images/ab300668-1-anti-cox2cyclooxygenase-2-antibody-33cox-2-immunocytochemistry-raw-2647.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-COX2 / Cyclooxygenase 2 antibody [33/Cox-2] (ab300668)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Raw 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling COX2 / Cyclooxygenase 2 with AB300668 at 1/50 diliution (19.94 µg/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green).
Confocal image showing increased cytoplasmic staining in Raw 264.7 cells treated with lipopolysaccharide (1µg/ml) for 6 hours.
ab202272 Recombinant Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab202272) was used to counterstain tubulin at 1/50 dilution (10 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml).
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![Anti-COX2 / Cyclooxygenase 2 antibody [33/Cox-2] (ab300668)](https://www.abcam.com/ps/products/300/ab300668/Images/ab300668-496305-BenefitsofRecombinantantibodies.jpg)
Anti-COX2 / Cyclooxygenase 2 antibody [33/Cox-2] (ab300668)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab300668 has not yet been referenced specifically in any publications.