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    products/primary-antibodies/cox2--cyclooxygenase-2-antibody-ep1978y-ab62331.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)

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  • SDS
Reviews (15) Submit a question References (70)

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Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
  • Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1978Y] to COX2 / Cyclooxygenase 2
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
Product image
Recombinant human COX2 / Cyclooxygenase 2 protein (ab198646)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Knockout
Product image
Human PTGS2 (COX2 / Cyclooxygenase 2) knockout HeLa cell line (ab255420)

View more associated products

Overview

  • Product name

    Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y]
    See all COX2 / Cyclooxygenase 2 primary antibodies
  • Description

    Rabbit monoclonal [EP1978Y] to COX2 / Cyclooxygenase 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab213704)

  • Positive control

    • WB: Raw264.7 cell lysate + LPS.
  • General notes

    Abcam recommends ab179800 for use in ICC/IF.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 1.01 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1978Y
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Blood
    • Platelets
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Prostaglandins
    • Cancer
    • Tumor biomarkers
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] - BSA and Azide free (ab221924)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Immunizing Peptide (Blocking)

    • COX2 / Cyclooxygenase 2 peptide (ab213704)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • RAW 264.7 whole cell lysate (ab7187)
  • Recombinant Protein

    • Recombinant human COX2 / Cyclooxygenase 2 protein (ab198646)
  • Related Products

    • Human PTGS2 (COX2 / Cyclooxygenase 2) knockout A549 cell line (ab280802)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab62331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (7)
1/500 - 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 69 kDa).Can be blocked with COX2 / Cyclooxygenase 2 peptide (ab213704).
Notes
WB
1/500 - 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 69 kDa).Can be blocked with COX2 / Cyclooxygenase 2 peptide (ab213704).
Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Function

    Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
  • Pathway

    Lipid metabolism; prostaglandin biosynthesis.
  • Sequence similarities

    Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • Post-translational
    modifications

    S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
  • Cellular localization

    Microsome membrane. Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession P35354 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5743 Human
    • Entrez Gene: 19225 Mouse
    • Omim: 600262 Human
    • SwissProt: P35354 Human
    • SwissProt: Q05769 Mouse
    • Unigene: 196384 Human
    • Unigene: 292547 Mouse
    • Alternative names

      • COX 2 antibody
      • COX-2 antibody
      • COX2 antibody
      • Cyclooxygenase 2 antibody
      • Cyclooxygenase 2b antibody
      • Cyclooxygenase antibody
      • Cyclooxygenase-2 antibody
      • Cyclooxygenase2 antibody
      • EC 1.14.99.1 antibody
      • fj02a10 antibody
      • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
      • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
      • GRIPGHS antibody
      • hCox 2 antibody
      • Macrophage activation-associated marker protein P71/73 antibody
      • OTTHUMP00000033524 antibody
      • PES-2 antibody
      • PGG/HS antibody
      • PGH synthase 2 antibody
      • PGH2_HUMAN antibody
      • PGHS 2 antibody
      • PGHS-2 antibody
      • PGHS2 antibody
      • PHS 2 antibody
      • PHS II antibody
      • PHS2 antibody
      • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin endoperoxide synthase 2 antibody
      • Prostaglandin G/H synthase 2 antibody
      • Prostaglandin G/H synthase 2 precursor antibody
      • Prostaglandin G/H synthase and cyclooxygenase antibody
      • Prostaglandin G/H synthase antibody
      • Prostaglandin H2 synthase 2 antibody
      • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin-endoperoxide synthase 2 antibody
      • PTGS2 antibody
      • ptgs2a antibody
      • TIS10 antibody
      • TIS10 protein antibody
      • unp1239 antibody
      • wu:fj02a10 antibody
      see all

    Images

    • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
      Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
      All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/500 dilution

      Lane 1 : Wild-type A549 cell lysate
      Lane 2 : PTGS2 knockout A549 cell lysate
      Lane 3 : U-87 MG cell lysate
      Lane 4 : MOLT-4 cell lysate
      Lane 5 : Wild-type Hela ab255929 cell lysate
      Lane 6 : PTGS2 knockout HeLa ab255524 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 69 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?



      False colour image of Western blot: Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab62331 was shown to bind specifically to COX2 / Cyclooxygenase 2. A band was observed at 75 kDa in wild-type A549 cell lysates with no signal observed at this size in PTGS2 knockout cell line ab280802 (knockout cell lysate ab283825). The band at 65 kDa is a unknown protein as a result of non-specific binding of the antibody. To generate this image, wild-type and PTGS2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
      Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
      All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/1000 dilution (purified)

      Lane 1 : Untreated Raw 264.7 cell lysate
      Lane 2 : Raw 264.7 treated with LPS

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG (H+L) at 1/50000 dilution

      Predicted band size: 69 kDa
      Observed band size: 72 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
      Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
      All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/1000 dilution (Purified)

      Lane 1 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
      Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
      Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution

      Predicted band size: 69 kDa
      Observed band size: 72 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure times

      Lane 1 and 2: 5.5 seconds
      Lane 3: 3.25 seconds

      We are unsure as to the
      identity of the extra band in HCT 116 and MCF7.
      The expression profile observed in HCT 116 and MCF7 are consistent with the literatures (PMID: 14739610, PMID: 24325753, PMID: 16997132).
      Negative control: HCT 116 (PMID: 14739610) and MCF7 (PMID: 24325753, PMID: 16997132)

    • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
      Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
      Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/500 dilution (unpurified) + Raw264.7 cell lysate (10ug) + LPS

      Secondary
      Goat anti-Rabbit HRP conjugated at 1/2000 dilution

      Predicted band size: 69 kDa
      Observed band size: 72 kDa why is the actual band size different from the predicted?

    • Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
      Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (70)

    Publishing research using ab62331? Please let us know so that we can cite the reference in this datasheet.

    ab62331 has been referenced in 70 publications.

    • Li YZ  et al. Silencing lncRNA SLC16A1-AS1 Induced Ferroptosis in Renal Cell Carcinoma Through miR-143-3p/SLC7A11 Signaling. Technol Cancer Res Treat 21:15330338221077803 (2022). PubMed: 35167383
    • Boyle AJ  et al. PET imaging of glycogen synthase kinase-3 in pancreatic cancer xenograft mouse models. Am J Nucl Med Mol Imaging 12:1-14 (2022). PubMed: 35295885
    • Liu S  et al. Inhibitory effect of lovastatin on human lung cancer cell proliferation by regulating the ERK1/2 and COX-2 pathways. Transl Cancer Res 11:813-822 (2022). PubMed: 35571660
    • Yin Y  et al. Protective Effect of Prim-O-Glucosylcimifugin on Ulcerative Colitis and Its Mechanism. Front Pharmacol 13:882924 (2022). PubMed: 35662727
    • Tao W  et al. Interaction between COX-2 and ER stress is involved in the apoptosis-induced myocardial ischemia/reperfusion injury. Am J Transl Res 14:3360-3371 (2022). PubMed: 35702111
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 15 Abreviews or Q&A

    Western blot abreview for Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Rat stomach)
    Gel Running Conditions
    Reduced Denaturing (Nupage BOLT gradient precast gel)
    Loading amount
    20 µg
    Treatment
    Carrageenan and indomethacin
    Specification
    Rat stomach
    Blocking step
    Biorad blocking buffer as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    PROF. Michele D'angelo

    Verified customer

    Submitted Oct 01 2021

    Western blot abreview for Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Zebrafish Tissue lysate - whole (tail fin)
    Gel Running Conditions
    Reduced Denaturing (4-12&)
    Loading amount
    20 µg
    Specification
    tail fin
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 17 2021

    Western blot abreview for Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Gastric Cancer Cell Line)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    3 µg
    Treatment
    LPS for 24 hrs
    Specification
    Gastric Cancer Cell Line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 11 2020

    Western blot abreview for Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse macrophage cell line)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    3 µg
    Specification
    Mouse macrophage cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Jared Sit

    Verified customer

    Submitted Nov 02 2020

    Western blot abreview for Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (melanoma cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    melanoma cell line
    Blocking step
    (agent) for 50 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. 令 中野

    Verified customer

    Submitted Jul 02 2019

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Robert Fenster

    Verified customer

    Submitted Aug 24 2018

    Western blot abreview for Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Baboon Tissue lysate - whole (Placenta samples and liver samples)
    Gel Running Conditions
    Reduced Denaturing (12% Gel)
    Loading amount
    40 µg
    Specification
    Placenta samples and liver samples
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kushal Gandhi

    Verified customer

    Submitted Sep 06 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
    Sample
    Dog Tissue sections (MDCK pellets in paraffin)
    Specification
    MDCK pellets in paraffin
    Permeabilization
    Yes - Tween-20
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 05 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y]

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Sample
    Mouse Cell (L cells)
    Specification
    L cells
    Permeabilization
    Yes - Triton 1%
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 10 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y]

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 1°C
    Sample
    Human Cell (293T)
    Specification
    293T
    Permeabilization
    Yes - Triton 1%
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 20 2014

    1-10 of 15 Abreviews or Q&A

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