Recombinant Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
![Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)](https://www.abcam.com/ps/products/62/ab62331/Images/ab62331-512517-anti-cox2-cyclooxygenase-2-antibody-ep1978y-western-blot-wildtype-a549-ptgs2-knockout-a549-u87-mg-molt4.jpg "Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1978Y] to COX2 / Cyclooxygenase 2
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y]
See all COX2 / Cyclooxygenase 2 primary antibodies -
Description
Rabbit monoclonal [EP1978Y] to COX2 / Cyclooxygenase 2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab213704) -
Positive control
- WB: Raw264.7 cell lysate + LPS.
-
General notes
Abcam recommends ab179800 for use in ICC/IF.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 1.01 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1978Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (7) |
1/500 - 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 69 kDa).Can be blocked with COX2 / Cyclooxygenase 2 peptide (ab213704).
|
| Notes |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 69 kDa).Can be blocked with COX2 / Cyclooxygenase 2 peptide (ab213704). |
Target
-
Function
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
Pathway
Lipid metabolism; prostaglandin biosynthesis. -
Sequence similarities
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
Post-translational
modificationsS-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
-
Database links
- Entrez Gene: 5743 Human
- Entrez Gene: 19225 Mouse
- Omim: 600262 Human
- SwissProt: P35354 Human
- SwissProt: Q05769 Mouse
- Unigene: 196384 Human
- Unigene: 292547 Mouse
-
Alternative names
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
Images
-
Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/500 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : PTGS2 knockout A549 cell lysate
Lane 3 : U-87 MG cell lysate
Lane 4 : MOLT-4 cell lysate
Lane 5 : Wild-type Hela ab255929 cell lysate
Lane 6 : PTGS2 knockout HeLa ab255524 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab62331 was shown to bind specifically to COX2 / Cyclooxygenase 2. A band was observed at 75 kDa in wild-type A549 cell lysates with no signal observed at this size in PTGS2 knockout cell line ab280802 (knockout cell lysate ab283825). The band at 65 kDa is a unknown protein as a result of non-specific binding of the antibody. To generate this image, wild-type and PTGS2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
-
![Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)](https://www.abcam.com/ps/products/62/ab62331/Images/ab62331-284754-anti-cox2-cyclooxygenase-2-antibody-ep1978y-western-blot.jpg)
Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/1000 dilution (purified)
Lane 1 : Untreated Raw 264.7 cell lysate
Lane 2 : Raw 264.7 treated with LPS
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 69 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST
-
![Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)](https://www.abcam.com/ps/products/62/ab62331/Images/ab62331-332354-anti-cox2-cyclooxygenase-2-antibody-ep1978y-western-blot.jpg)
Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/1000 dilution (Purified)
Lane 1 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution
Predicted band size: 69 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times
Lane 1 and 2: 5.5 seconds
Lane 3: 3.25 secondsWe are unsure as to the
identity of the extra band in HCT 116 and MCF7.
The expression profile observed in HCT 116 and MCF7 are consistent with the literatures (PMID: 14739610, PMID: 24325753, PMID: 16997132).
Negative control: HCT 116 (PMID: 14739610) and MCF7 (PMID: 24325753, PMID: 16997132) -
![Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)](https://www.abcam.com/ps/products/62/ab62331/Images/ab62331-75633-anti-cox2-cyclooxygenase-2-antibody-ep1978y-western-blot.jpg)
Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/500 dilution (unpurified) + Raw264.7 cell lysate (10ug) + LPS
Secondary
Goat anti-Rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 69 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted? -
![Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)](https://www.abcam.com/ps/products/62/ab62331/Images/ab62331-9-benefits-of-recombinant-antibodies.png)
Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (70)
ab62331 has been referenced in 70 publications.
- Li YZ et al. Silencing lncRNA SLC16A1-AS1 Induced Ferroptosis in Renal Cell Carcinoma Through miR-143-3p/SLC7A11 Signaling. Technol Cancer Res Treat 21:15330338221077803 (2022). PubMed: 35167383
- Boyle AJ et al. PET imaging of glycogen synthase kinase-3 in pancreatic cancer xenograft mouse models. Am J Nucl Med Mol Imaging 12:1-14 (2022). PubMed: 35295885
- Liu S et al. Inhibitory effect of lovastatin on human lung cancer cell proliferation by regulating the ERK1/2 and COX-2 pathways. Transl Cancer Res 11:813-822 (2022). PubMed: 35571660
- Yin Y et al. Protective Effect of Prim-O-Glucosylcimifugin on Ulcerative Colitis and Its Mechanism. Front Pharmacol 13:882924 (2022). PubMed: 35662727
- Tao W et al. Interaction between COX-2 and ER stress is involved in the apoptosis-induced myocardial ischemia/reperfusion injury. Am J Transl Res 14:3360-3371 (2022). PubMed: 35702111