Recombinant Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)
![Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)](https://www.abcam.com/ps/products/169/ab169782/Images/ab169782-501338-ab169782anticox2westernblothuman.jpg "Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP8588] to COX2 / Cyclooxygenase 2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-COX2 / Cyclooxygenase 2 antibody [EP8588]
See all COX2 / Cyclooxygenase 2 primary antibodies -
Description
Rabbit monoclonal [EP8588] to COX2 / Cyclooxygenase 2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep, Rabbit, Horse, Cow, Mink
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A549, U-87 MG, Wild-type HeLa and PTGS2 knockout HeLa cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP8588 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
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| Notes |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 69 kDa. |
Target
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Function
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
Pathway
Lipid metabolism; prostaglandin biosynthesis. -
Sequence similarities
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
Post-translational
modificationsS-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 282023 Cow
- Entrez Gene: 5743 Human
- Entrez Gene: 443460 Sheep
- Omim: 600262 Human
- SwissProt: O62698 Cow
- SwissProt: O19183 Horse
- SwissProt: P35354 Human
- SwissProt: O02768 Rabbit
see all -
Alternative names
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
Images
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Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782) at 1/5000 dilution
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 69 kDa
Exposure time: 10 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Primary antibody: incubated for 1h at room temperature.
Secondary antibody: incubated for 1h at room temperature.Negative control: MCF7 (PMID: 24325753, PMID: 16997132).
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![Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)](https://www.abcam.com/ps/products/169/ab169782/Images/ab169782-4-anti-cox2-cyclooxygenase-2-antibody-ep8588-western-blot-human-lysate.jpg)
Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : U-87 MG cell lysate
Lane 3 : Wild-type HeLa cell lysate
Lane 4 : PTGS2 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 69 kDaLanes 1 - 4: Merged signal (red and green). Green - ab169782 observed at 75 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab169782 was shown to react with COX2 / Cyclooxygenase 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255420 (knockout cell lysate ab263795) was used. Wild-type and COX2 / Cyclooxygenase 2 knockout samples were subjected to SDS-PAGE. ab169782 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
This image was obtained using the hybridoma version of ab169782.
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![Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)](https://www.abcam.com/ps/products/169/ab169782/Images/ab169782-168451-anti-cox2-cyclooxygenase-2-antibody-ep8588-western-blot.jpg)
Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : Lysate from THP1 cells treated with LPS
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 69 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab169782 has been referenced in 4 publications.
- Zhu L et al. Identification the ferroptosis-related gene signature in patients with esophageal adenocarcinoma. Cancer Cell Int 21:124 (2021). PubMed: 33602233
- Wang J et al. Involvement of the VEGF signaling pathway in immunosuppression and hypoxia stress: analysis of mRNA expression in lymphocytes mediating panting in Jersey cattle under heat stress. BMC Vet Res 17:209 (2021). PubMed: 34098948
- Zhang H et al. Long-term use of fluoxetine accelerates bone loss through the disruption of sphingolipids metabolism in bone marrow adipose tissue. Transl Psychiatry 10:138 (2020). PubMed: 32398744
- Jin J et al. Pirfenidone attenuates lung fibrotic fibroblast responses to transforming growth factor-ß1. Respir Res 20:119 (2019). PubMed: 31185973