Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
Key features and details
- Mouse monoclonal [10G8D12C12] to COX4 + COX4L2
- Suitable for: IHC-Fr, WB, Flow Cyt, ICC/IF
- Reacts with: Cow, Human
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Anti-COX4 + COX4L2 antibody [10G8D12C12]
See all COX4 + COX4L2 primary antibodies -
Description
Mouse monoclonal [10G8D12C12] to COX4 + COX4L2 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, WB, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Cow, Human
Predicted to work with: Zebrafish -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Isolated mitochondria from Human heart, Bovine heart and HepG2 cells. Human skeletal muscle (frozen sections). Cultured Human embryonic lung derived fibroblasts (strain MRC5; fixed and permeabilized). Human colon tissue sections. HeLa cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
ab110261 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab110261 was judged as near homogeneity by SDS PAGE. -
Clonality
Monoclonal -
Clone number
10G8D12C12 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
1/800.
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WB | (2) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa.
|
Flow Cyt |
Use a concentration of 1 µg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
ICC/IF |
Use a concentration of 5 µg/ml.
(heat-induced antigen-retrieval improves signal) |
Notes |
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IHC-Fr
1/800. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use a concentration of 5 µg/ml. (heat-induced antigen-retrieval improves signal) |
Target
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Relevance
Cytochrome C Oxidase is located in the inner mitochondrial membrane and is the terminal enzyme complex of the mitochondrial electron transport chain. It collects electrons that are transferred from reduced cytochrome C and donates them to molecular oxygen, which is then reduced to water. It is composed of cytochrome A and cytochrome B, two copper atoms, and 13 different protein subunits, three of which are encoded by the mitochondrial DNA and ten others by nuclear DNA (mammals). -
Cellular localization
Mitochondrial inner membrane -
Database links
- Entrez Gene: 1327 Human
- Entrez Gene: 84701 Human
- Omim: 123864 Human
- Omim: 607976 Human
- SwissProt: P13073 Human
- SwissProt: Q96KJ9 Human
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Alternative names
- AL024441 antibody
- COX 4 antibody
- COX IV antibody
see all
Images
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All lanes : Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261) at 1 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
Lane 3 : Isolated mitochondria from HepG2 at 20 µg
Predicted band size: 20 kDa -
ab110261, at 1 µg/mL, staining COX4 + COX4L2 in HeLa cells by Immunohistochemistry (blue) and an isotype control antibody, at 1 µg/mL, staining of HeLa cells (red).
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ab110261, at 10 µg/ml, staining COX4 + COX4L2 in cultured Human embryonic lung derived fibroblasts by Immunofluorescence. The cells (strain MRC5) were fixed, permeabilized and then labeled with ab110261 followed by an AlexaFluor® 488 conjugated Goat anti-mouse IgG2a isotype specific secondary antibody at 1 µg/ml.
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ab110261, at 1/800, staining COX4 + COX4L2 in skeletal muscle tissue sections from a patient with a single large deletion of the mtDNA by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.
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ab110261, at 1/200, showing COX4 + COX4L2 negative crypts in colon tissue sections from a normal ageing patient by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (48)
ab110261 has been referenced in 48 publications.
- Erskine D et al. Lipids, lysosomes and mitochondria: insights into Lewy body formation from rare monogenic disorders. Acta Neuropathol 141:511-526 (2021). PubMed: 33515275
- Frazier AE et al. Fatal perinatal mitochondrial cardiac failure caused by recurrent de novo duplications in the ATAD3 locus. Med (N Y) 2:49-73 (2021). PubMed: 33575671
- Dibley MG et al. The Mitochondrial Acyl-carrier Protein Interaction Network Highlights Important Roles for LYRM Family Members in Complex I and Mitoribosome Assembly. Mol Cell Proteomics 19:65-77 (2020). PubMed: 31666358
- Formosa LE et al. Dissecting the Roles of Mitochondrial Complex I Intermediate Assembly Complex Factors in the Biogenesis of Complex I. Cell Rep 31:107541 (2020). PubMed: 32320651
- Warren C et al. Decoding mitochondrial heterogeneity in single muscle fibres by imaging mass cytometry. Sci Rep 10:15336 (2020). Human . PubMed: 32948797