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    products/primary-antibodies/cox4--cox4l2-antibody-10g8d12c12-ab110261.pdf

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Metabolism Pathways and Processes Mitochondrial Metabolism Mitochondrial markers
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Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)

  • Datasheet
  • SDS
Reviews (2)Q&A (4)References (48)

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Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
  • Flow Cytometry - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
  • Immunocytochemistry/ Immunofluorescence - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
  • Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
  • Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)

Key features and details

  • Mouse monoclonal [10G8D12C12] to COX4 + COX4L2
  • Suitable for: IHC-Fr, WB, Flow Cyt, ICC/IF
  • Reacts with: Cow, Human
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647

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Overview

  • Product name

    Anti-COX4 + COX4L2 antibody [10G8D12C12]
    See all COX4 + COX4L2 primary antibodies
  • Description

    Mouse monoclonal [10G8D12C12] to COX4 + COX4L2
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Cow, Human
    Predicted to work with: Zebrafish
  • Immunogen

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Isolated mitochondria from Human heart, Bovine heart and HepG2 cells. Human skeletal muscle (frozen sections). Cultured Human embryonic lung derived fibroblasts (strain MRC5; fixed and permeabilized). Human colon tissue sections. HeLa cells.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.5
    Preservative: 0.02% Sodium azide
    Constituent: HEPES buffered saline
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    ab110261 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab110261 was judged as near homogeneity by SDS PAGE.
  • Clonality

    Monoclonal
  • Clone number

    10G8D12C12
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex IV

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab198318)
    • Alexa Fluor® 488 Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab198590)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
1/800.
WB (2)
Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF
Use a concentration of 5 µg/ml.

(heat-induced antigen-retrieval improves signal)

Notes
IHC-Fr
1/800.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF
Use a concentration of 5 µg/ml.

(heat-induced antigen-retrieval improves signal)

Target

  • Relevance

    Cytochrome C Oxidase is located in the inner mitochondrial membrane and is the terminal enzyme complex of the mitochondrial electron transport chain. It collects electrons that are transferred from reduced cytochrome C and donates them to molecular oxygen, which is then reduced to water. It is composed of cytochrome A and cytochrome B, two copper atoms, and 13 different protein subunits, three of which are encoded by the mitochondrial DNA and ten others by nuclear DNA (mammals).
  • Cellular localization

    Mitochondrial inner membrane
  • Database links

    • Entrez Gene: 1327 Human
    • Entrez Gene: 84701 Human
    • Omim: 123864 Human
    • Omim: 607976 Human
    • SwissProt: P13073 Human
    • SwissProt: Q96KJ9 Human
    • Alternative names

      • AL024441 antibody
      • COX 4 antibody
      • COX IV antibody
      • Cox4 antibody
      • Cox4a antibody
      • COX4B antibody
      • COX4I1 antibody
      • COX4I2 antibody
      • COX4L2 antibody
      • COXIV antibody
      • Cytochrome c oxidase polypeptide IV antibody
      • Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody
      • Cytochrome C Oxidase subunit IV antibody
      • Cytochrome c oxidase subunit IV isoform 1 antibody
      • Cytochrome c oxidase subunit IV isoform 2 (lung) antibody
      • Cytochrome c oxydase subunit 4 antibody
      • dJ857M17.2 antibody
      • MGC105470 antibody
      • MGC72016 antibody
      see all

    Images

    • Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
      Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
      All lanes : Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261) at 1 µg/ml

      Lane 1 : Isolated mitochondria from Human heart at 5 µg
      Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
      Lane 3 : Isolated mitochondria from HepG2 at 20 µg

      Predicted band size: 20 kDa

    • Flow Cytometry - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
      Flow Cytometry - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
      ab110261, at 1 µg/mL, staining COX4 + COX4L2 in HeLa cells by Immunohistochemistry (blue) and an isotype control antibody, at 1 µg/mL, staining of HeLa cells (red).
    • Immunocytochemistry/ Immunofluorescence - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
      Immunocytochemistry/ Immunofluorescence - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
      ab110261, at 10 µg/ml, staining COX4 + COX4L2 in cultured Human embryonic lung derived fibroblasts by Immunofluorescence. The cells (strain MRC5) were fixed, permeabilized and then labeled with ab110261 followed by an AlexaFluor® 488 conjugated Goat anti-mouse IgG2a isotype specific secondary antibody at 1 µg/ml.
    • Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
      Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
      ab110261, at 1/800, staining COX4 + COX4L2 in skeletal muscle tissue sections from a patient with a single large deletion of the mtDNA by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.
    • Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
      Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
      ab110261, at 1/200, showing COX4 + COX4L2 negative crypts in colon tissue sections from a normal ageing patient by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (48)

    Publishing research using ab110261? Please let us know so that we can cite the reference in this datasheet.

    ab110261 has been referenced in 48 publications.

    • Erskine D  et al. Lipids, lysosomes and mitochondria: insights into Lewy body formation from rare monogenic disorders. Acta Neuropathol 141:511-526 (2021). PubMed: 33515275
    • Frazier AE  et al. Fatal perinatal mitochondrial cardiac failure caused by recurrent de novo duplications in the ATAD3 locus. Med (N Y) 2:49-73 (2021). PubMed: 33575671
    • Dibley MG  et al. The Mitochondrial Acyl-carrier Protein Interaction Network Highlights Important Roles for LYRM Family Members in Complex I and Mitoribosome Assembly. Mol Cell Proteomics 19:65-77 (2020). PubMed: 31666358
    • Formosa LE  et al. Dissecting the Roles of Mitochondrial Complex I Intermediate Assembly Complex Factors in the Biogenesis of Complex I. Cell Rep 31:107541 (2020). PubMed: 32320651
    • Warren C  et al. Decoding mitochondrial heterogeneity in single muscle fibres by imaging mass cytometry. Sci Rep 10:15336 (2020). Human . PubMed: 32948797
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-COX4 + COX4L2 antibody [10G8D12C12 ]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (Reduced Denaturing (Reduced Denaturing (4-15%))
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 09 2012

    Western blot abreview for Anti-COX4 + COX4L2 antibody [10G8D12C12 ]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (Isolated Mouse Liver Mitochondria)
    Loading amount
    20 µg
    Specification
    Isolated Mouse Liver Mitochondria
    Gel Running Conditions
    Reduced Denaturing (10% Bis Tris)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 05 2011

    Question

    thank you very much for your prompt reply.
    I am referring to yhe antibody ab 110261.
    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Dec 14 2012

    Answer

    Thank you for confirming the product code number.

    This antibody has been tested for immunohistochemistry by one of our collaborators. The optimal dilution varies depending on tissue types but 5ug/mL is recommended as a good starting point.

    All immunohistochemical procedures were performed on formalin fixed paraffin-embedded (FFPE) cerebellar tissue sectioned at a thickness of 5 μm (length of formalin fixation < 6 months). Tissue was obtained from an aged human control subject.

    Following deparaffinisation and rehydration of tissue sections, antigen retrieval was performed either by the Decloaker (Menapath, UK: this is very much like a pressure cooker) in 1 mmol EDTA pH 8 or x2 5 minutes microwaving in 0.1 mmol citrate pH 6.

    Subsequent to this, sections were incubated in 3% hydrogen peroxide for 20 minutes to block endogenous peroxide and then incubated with the primary antibody at a range of dilutions (see the information provided in the Legend of the on-line IHC-P images) for 1 hour at room temperature.

    Following this, the signal of the primary antibody binding was developed using the polymer-detection system (Menapath kit) and visualized with DAB. Sections were dehydrated and mounted in DPX to allow for examination under the microscope.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Dec 14 2012

    Question

    I need the MSDS for the following products ordered today with order number:

    Anti-GRIM19 antibody [6E1BH7] (ab110240)
    Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
    Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)
    Anti-SDHB antibody [21A11AE7] (ab14714)
    Anti-SDHA antibody [2E3GC12FB2AE2] - Mitochondrial Marker (ab14715)
    Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)
    Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)
    Anti-FH antibody [8F12BB5] (ab110286)
    Anti-Pyruvate Dehydrogenase E2 antibody [15D3G9C11 ] (ab110332)

    Read More

    Abcam community

    Verified customer

    Asked on Nov 23 2012

    Answer

    Thank you for contacting us.

    The MSDS for these products have been added to each product datasheet. They should appear on the website within the next few hours (time for the cache to be regenerated).

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Nov 23 2012

    Question

    Klone heißen anders/kürzer:
    ab14744 : 20E8
    ab14705 : 1D6E
    Kundin hat diese AKs am 10.1.2011 bestellt, kamen als Lyophilisat und wurden eingefroren bei -20C
    Ist das okay?
    Sind die Klone dieselben?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 21 2012

    Answer

    Vielen Dank für Ihren Anruf.

    Ich freue mich Ihnen bestätigen zu können, dass die Klone dieselben sind und früher bei MitoScience nur abgekürzt wurden.

    Ich kann Ihnen auch bestätigen, dass eine Lagerung bei -20C korrekt ist solange die Antikörper lyophilisiert sind.

    Bitte lagern sie die Antikörper bei +4C nach Rekonstitution.

    Read More

    Abcam Scientific Support

    Answered on Nov 21 2012

    Question

    Kindly advise if ab110261, ab 63947 will cross react with mouse samples (for WB).

    Read More

    Abcam community

    Verified customer

    Asked on Mar 16 2012

    Answer

    Thank you for contacting us.
    Unfortunately, ab110261 has been tested and found to be unsuitable for use on mouse samples, and ab63947 was raised against a very short immunogen that shows only 69% homology with mouse. We would not expect either of these antibodies to give good results for mouse samples.
    If you are looking for an antibody to COX subunit IV, ab14744 works well in a number of applications and species, including mouse and WB. I hope this helps, please let me know if you need any additional information or assistance.

    Read More

    Abcam Scientific Support

    Answered on Mar 16 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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