Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)

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This antibody has been tested for immunohistochemistry by one of our collaborators. The optimal dilution varies depending on tissue types but 5ug/mL is recommended as a good starting point.

All immunohistochemical procedures were performed on formalin fixed paraffin-embedded (FFPE) cerebellar tissue sectioned at a thickness of 5 μm (length of formalin fixation < 6 months). Tissue was obtained from an aged human control subject.

Following deparaffinisation and rehydration of tissue sections, antigen retrieval was performed either by the Decloaker (Menapath, UK: this is very much like a pressure cooker) in 1 mmol EDTA pH 8 or x2 5 minutes microwaving in 0.1 mmol citrate pH 6.

Subsequent to this, sections were incubated in 3% hydrogen peroxide for 20 minutes to block endogenous peroxide and then incubated with the primary antibody at a range of dilutions (see the information provided in the Legend of the on-line IHC-P images) for 1 hour at room temperature.

Following this, the signal of the primary antibody binding was developed using the polymer-detection system (Menapath kit) and visualized with DAB. Sections were dehydrated and mounted in DPX to allow for examination under the microscope.

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Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Ich freue mich Ihnen bestätigen zu können, dass die Klone dieselben sind und früher bei MitoScience nur abgekürzt wurden.

Ich kann Ihnen auch bestätigen, dass eine Lagerung bei -20C korrekt ist solange die Antikörper lyophilisiert sind.

Bitte lagern sie die Antikörper bei +4C nach Rekonstitution.

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Unfortunately, ab110261 has been tested and found to be unsuitable for use on mouse samples, and ab63947 was raised against a very short immunogen that shows only 69% homology with mouse. We would not expect either of these antibodies to give good results for mouse samples.
If you are looking for an antibody to COX subunit IV, ab14744 works well in a number of applications and species, including mouse and WB. I hope this helps, please let me know if you need any additional information or assistance.