Anti-COX4, mitochondrial antibody [1A12A12] (ab110272)
![Western blot - Anti-COX4, mitochondrial antibody [1A12A12] (ab110272)](https://www.abcam.com/ps/products/110/ab110272/Images/ab110272-123465-anti-cytochrome-c-oxidase-subunit-4-mitochondrial-antibody-1a12a12-western-blot.jpg "Western blot - Anti-COX4, mitochondrial antibody [1A12A12] (ab110272)")
Key features and details
- Mouse monoclonal [1A12A12] to COX4, mitochondrial
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
Overview
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Product name
Anti-COX4, mitochondrial antibody [1A12A12] -
Description
Mouse monoclonal [1A12A12] to COX4, mitochondrial -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- Mitochondria from yeast membrane extract
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Near homogeneity as judged by SDS-PAGE. ab110272 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
1A12A12 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
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| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa. |
Target
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Sequence similarities
Belongs to the cytochrome c oxidase subunit 5B family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 852688 Saccharomyces cerevisiae
- SwissProt: P04037 Saccharomyces cerevisiae
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Alternative names
- cox4 antibody
- COX4 Cox4p antibody
- COX4_YEAST antibody
see all
Images
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Western blot - Anti-COX4, mitochondrial antibody [1A12A12] (ab110272)Anti-COX4, mitochondrial antibody [1A12A12] (ab110272) at 1 µg/ml + Mitochondria from yeast membrane extract at 20 µg
Predicted band size: 17 kDa
Datasheets and documents
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Datasheet download
References (18)
ab110272 has been referenced in 18 publications.
- Chen C et al. Imaging mass cytometry reveals generalised deficiency in OXPHOS complexes in Parkinson's disease. NPJ Parkinsons Dis 7:39 (2021). PubMed: 33980828
- Chang HC et al. Augmenter of liver regeneration regulates cellular iron homeostasis by modulating mitochondrial transport of ATP-binding cassette B8. Elife 10:N/A (2021). PubMed: 33835027
- Duan C et al. Drp1 regulates mitochondrial dysfunction and dysregulated metabolism in ischemic injury via Clec16a-, BAX-, and GSH- pathways. Cell Death Dis 11:251 (2020). PubMed: 32312970
- Ghosh S et al. An essential role for cardiolipin in the stability and function of the mitochondrial calcium uniporter. Proc Natl Acad Sci U S A 117:16383-16390 (2020). PubMed: 32601238
- Buso A et al. Mitochondrial Adaptations in Elderly and Young Men Skeletal Muscle Following 2 Weeks of Bed Rest and Rehabilitation. Front Physiol 10:474 (2019). PubMed: 31118897
