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Anti-COX5A antibody [6E9B12D5] (ab110262)

Submit a review Q&A (4)References (50)
Western blot - Anti-COX5A antibody [6E9B12D5] (ab110262)
  • Immunocytochemistry/ Immunofluorescence - Anti-COX5A antibody [6E9B12D5] (ab110262)
  • Flow Cytometry - Anti-COX5A antibody [6E9B12D5] (ab110262)

Key features and details

  • Mouse monoclonal [6E9B12D5] to COX5A
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-COX5A antibody [6E9B12D5]
    See all COX5A primary antibodies

  • Description

    Mouse monoclonal [6E9B12D5] to COX5A

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human

  • Immunogen

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, and HepG2, Human embryonic lung-derived fibroblasts (strain MRC5), HeLa cells

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.5
    Preservative: 0.02% Sodium azide
    Constituent: HEPES buffered saline
  • Concentration information loading...

  • Purification notes

    ab110262 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

  • Clonality

    Monoclonal

  • Clone number

    6E9B12D5

  • Isotype

    IgG2a

  • Light chain type

    kappa

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
ICC/IF
Use a concentration of 5 µg/ml.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
ICC/IF
Use a concentration of 5 µg/ml.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

Images

  • Western blot - Anti-COX5A antibody [6E9B12D5] (ab110262)
    Western blot - Anti-COX5A antibody [6E9B12D5] (ab110262)
    All lanes : Anti-COX5A antibody [6E9B12D5] (ab110262) at 1 µg/ml

    Lane 1 : Human heart mitochondria at 5 µg
    Lane 2 : Bovine heart mitochondria at 1 µg
    Lane 3 : Rat heart mitochondria at 10 µg
    Lane 4 : Mouse heart mitochondria at 10 µg
    Lane 5 : HepG2 mitochondria at 20 µg

    Predicted band size: 17 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-COX5A antibody [6E9B12D5] (ab110262)
    Immunocytochemistry/ Immunofluorescence - Anti-COX5A antibody [6E9B12D5] (ab110262)
    Mitochondrial localization of COX5A. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110262 (10 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody (1 µg/ml).
  • Flow Cytometry - Anti-COX5A antibody [6E9B12D5] (ab110262)
    Flow Cytometry - Anti-COX5A antibody [6E9B12D5] (ab110262)
    HeLa cells were stained with 1 µg/mL ab110262 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

References (50)

Publishing research using ab110262? Please let us know so that we can cite the reference in this datasheet.

ab110262 has been referenced in 50 publications.

  • Li K  et al. Maternally Inherited Diabetes Mellitus Associated with a Novel m.15897G>A Mutation in Mitochondrial tRNAThr Gene. J Diabetes Res 2020:2057187 (2020). PubMed: 32083134
  • Protasoni M  et al. Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV. EMBO J 39:e102817 (2020). PubMed: 31912925
  • Lobo-Jarne T  et al. Multiple pathways coordinate assembly of human mitochondrial complex IV and stabilization of respiratory supercomplexes. EMBO J N/A:e103912 (2020). PubMed: 32511785
  • Pajuelo Reguera D  et al. Cytochrome c Oxidase Subunit 4 Isoform Exchange Results in Modulation of Oxygen Affinity. Cells 9:N/A (2020). PubMed: 32075102
  • Chong SJF  et al. Serine-70 phosphorylated Bcl-2 prevents oxidative stress-induced DNA damage by modulating the mitochondrial redox metabolism. Nucleic Acids Res 48:12727-12745 (2020). PubMed: 33245769
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Question

Thank you, we look forward to receiving the replacement. The lot you have available, GR47109-2, will be fine.

Read More
Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab110261.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

Read More

Question

Dear Technical Support/Custom Service,



We purchased ab110262 on March 26, 2012, and unfortunately it did not perform in our experiments. Please see attached image. Would it be possible to receive a substitution/replacement (MS408 COXIV lot B0659)?

Read More
Answer

Thanks for sending the western blot data to my Outlook account. I agree it is very strange that the powerpoint file somehow turned into one of our datasheets!
I agree that the vial of ab110262 you received is not working as expcted. I am happy to send a vial of MS408. This corresponds to catalog number ab110261. The current lot we have in stock is GR47109-2. Please let me know if this is OK and I'll send out this replacement immediately.
I look forward to hearing from you.

Read More

Question

Please see the attached image of another experiment with a negative
result that we obtained with Ab110262 COX5a lot GR73589-1. Moreover, we don't
need to strip the membranes prior to the second blot (with CORE2), since
the two proteins have two very different molecular weights.
For this reason, could you please send us in substitution the antibody COXIV
MS408, lot B0659?
Thank you very much

Read More
Answer

Thanks for your reply.
Oddly, the powerpoint attachement opens up to show an image of one of our datasheets, for ab110262. Not sure what the cause is but if you could, please forward the powerpoint image to my outlook account.

Read More

Question

Dear Technical Support/Custom Service,



We purchased ab110262 on March 26, 2012 (Order No. 1059903), and unfortunately it did not perform in our experiments. Please see attached image. Would it be possible to receive a substitution/replacement (MS408 COXIV lot B0659)?

Read More
Answer

Thank you for your enquiry. I am sorry to hear this vial of ab110262 is giving you trouble.
And thanks for including the western blot images. If I understand you correctly, you first probed the membrane with anti-CORE2 antibody and then probed the same membrane with COXVA antibody. It appears that the essentially the same signal is showing through on the COXVA staining as was present on the CORE2 staining.
I think the first blot was not completely stripped of signal. So the second blot is difficult to interpret.
I would recommend running a new gel and blotting for COXVA.
I hope this is helpful and I look forward to hearing if these suggestions prove useful.

Read More

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