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Anti-COX5B antibody [16H12H9] (ab110263)

Submit a review Submit a question References (15)
Western blot - Anti-COX5B antibody [16H12H9] (ab110263)
  • Flow Cytometry - Anti-COX5B antibody [16H12H9] (ab110263)

Key features and details

  • Mouse monoclonal [16H12H9] to COX5B
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-COX5B antibody [16H12H9]
    See all COX5B primary antibodies

  • Description

    Mouse monoclonal [16H12H9] to COX5B

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • Isolated mitochondria from Human heart, Bovine heart, Rat heart, and Mouse heart

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. 
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 14 kDa.
Notes
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. 
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 14 kDa.

Target

Images

  • Western blot - Anti-COX5B antibody [16H12H9] (ab110263)
    Western blot - Anti-COX5B antibody [16H12H9] (ab110263)
    All lanes : Anti-COX5B antibody [16H12H9] (ab110263) at 1 µg/ml

    Lane 1 : Human heart mitochondria at 5 µg
    Lane 2 : Bovine heart mitochondria at 1 µg
    Lane 3 : Rat heart mitochondria at 10 µg
    Lane 4 : Mouse heart mitochondria at 10 µg

    Predicted band size: 14 kDa

  • Flow Cytometry - Anti-COX5B antibody [16H12H9] (ab110263)
    Flow Cytometry - Anti-COX5B antibody [16H12H9] (ab110263)
    Overlay histogram showing HepG2 cells stained with ab110263 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110263, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References (15)

Publishing research using ab110263? Please let us know so that we can cite the reference in this datasheet.

ab110263 has been referenced in 15 publications.

  • Lee CQE  et al. Coding and non-coding roles of MOCCI (C15ORF48) coordinate to regulate host inflammation and immunity. Nat Commun 12:2130 (2021). PubMed: 33837217
  • Signes A  et al. APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS. EMBO Mol Med 11:N/A (2019). PubMed: 30552096
  • Ghosh J  et al. Cigarette Smoke Toxins-Induced Mitochondrial Dysfunction and Pancreatitis Involves Aryl Hydrocarbon Receptor Mediated Cyp1 Gene Expression: Protective Effects of Resveratrol. Toxicol Sci 166:428-440 (2018). PubMed: 30165701
  • Hasenan SM  et al. Modulation of age related protein expression changes by gelam honey in cardiac mitochondrial rats. Exp Gerontol 113:1-9 (2018). PubMed: 30248357
  • Anandasadagopan SK  et al. ß -Naphthoflavone-Induced Mitochondrial Respiratory Damage in Cyp1 Knockout Mouse and in Cell Culture Systems: Attenuation by Resveratrol Treatment. Oxid Med Cell Longev 2017:5213186 (2017). PubMed: 29098061
View all Publications for this product

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