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    products/primary-antibodies/cox6a2-antibody-4h2a5-ab110264.pdf

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Anti-COX6A2 antibody [4H2A5] (ab110264)

  • Datasheet
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Western blot - Anti-COX6A2 antibody [4H2A5] (ab110264)

    Key features and details

    • Mouse monoclonal [4H2A5] to COX6A2
    • Suitable for: WB
    • Reacts with: Cow
    • Isotype: IgG2b

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    Overview

    • Product name

      Anti-COX6A2 antibody [4H2A5]
      See all COX6A2 primary antibodies
    • Description

      Mouse monoclonal [4H2A5] to COX6A2
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Cow
    • Immunogen

      Full length native protein (purified). This information is considered to be commercially sensitive.

    • Positive control

      • Isolated mitochondria from bovine heart
    • General notes

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

      Product was previously marketed under the MitoSciences sub-brand.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Do Not Freeze.
    • Storage buffer

      pH: 7.5
      Preservative: 0.02% Sodium azide
      Constituent: HEPES buffered saline
    • Concentration information loading...
    • Purity

      IgG fraction
    • Purification notes

      Near homogeneity as judged by SDS-PAGE. ab110264 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
    • Clonality

      Monoclonal
    • Clone number

      4H2A5
    • Isotype

      IgG2b
    • Light chain type

      kappa
    • Research areas

      • Tags & Cell Markers
      • Subcellular Markers
      • Organelles
      • Mitochondria
      • Signal Transduction
      • Metabolism
      • Mitochondrial
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitochondrial markers
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Oxidative phosphorylation
      • Complex IV
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab110264 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 25 µg/ml. Predicted molecular weight: 11 kDa.
    Notes
    WB
    Use a concentration of 25 µg/ml. Predicted molecular weight: 11 kDa.

    Target

    • Relevance

      Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. COX is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. COX6A2 is polypeptide 2 of subunit VIa, and is present only in striated muscles. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex.
    • Cellular localization

      Mitochondrion inner membrane
    • Database links

      • Entrez Gene: 282200 Cow
      • SwissProt: P07471 Cow
      • Alternative names

        • COX VIa M antibody
        • COX6AH antibody
        • COXVIAH antibody
        • Cytochrome c oxidase polypeptide VIa heart antibody
        • Cytochrome c oxidase subunit 6A2 mitochondrial antibody
        • Cytochrome c oxidase subunit VIA muscle antibody
        • Cytochrome c oxidase subunit VIa polypeptide 2 antibody
        • MS411 antibody
        see all

      Images

      • Western blot - Anti-COX6A2 antibody [4H2A5] (ab110264)
        Western blot - Anti-COX6A2 antibody [4H2A5] (ab110264)
        Anti-COX6A2 antibody [4H2A5] (ab110264) at 25 µg/ml + Isolated mitochondria from bovine heart at 1 µg

        Predicted band size: 11 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (0)

      Publishing research using ab110264? Please let us know so that we can cite the reference in this datasheet.

      ab110264 has not yet been referenced specifically in any publications.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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