Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)
![Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)](https://www.abcam.com/ps/products/110/ab110266/Images/ab110266-196280-anti-cytochrome-c-oxidase-subunit-vib-antibody-3f9d3d11af6-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)")
Key features and details
- Mouse monoclonal [3F9D3D11AF6] to COX6B1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-COX6B1 antibody [3F9D3D11AF6]
See all COX6B1 primary antibodies -
Description
Mouse monoclonal [3F9D3D11AF6] to COX6B1 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human, cow, rat and mouse heart. IF/ICC: HeLa cell line.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purification notes
Near homogeneity as judged by SDS-PAGE. ab110266 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
3F9D3D11AF6 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa.
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| Notes |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa. |
Target
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Function
Connects the two COX monomers into the physiological dimeric form. -
Involvement in disease
Defects in COX6B1 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome. -
Sequence similarities
Belongs to the cytochrome c oxidase subunit 6B family. -
Cellular localization
Mitochondrion intermembrane space. - Information by UniProt
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Database links
- Entrez Gene: 100270792 Cow
- Entrez Gene: 1340 Human
- Entrez Gene: 110323 Mouse
- Entrez Gene: 502592 Rat
- Omim: 124089 Human
- SwissProt: P00429 Cow
- SwissProt: P14854 Human
- SwissProt: P56391 Mouse
see all -
Alternative names
- COX 6B antibody
- COX VIb 1 antibody
- COX VIb-1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)
ICC/IF image of ab110266 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110266, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Western blot - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)](https://www.abcam.com/ps/products/110/ab110266/Images/ab110266-123458-anti-cytochrome-c-oxidase-subunit-vib-antibody-3f9d3d11af6-western-blot.jpg)
Western blot - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)All lanes : Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266) at 1 µg/ml
Lane 1 : Isolated mitochondria from Human Heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine Heart at 4 µg
Lane 3 : Isolated mitochondria from Rat Heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse Heart at 10 µg
Predicted band size: 10 kDa
Protocols
Datasheets and documents
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Datasheet download
References (12)
ab110266 has been referenced in 12 publications.
- Protasoni M et al. Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV. EMBO J 39:e102817 (2020). PubMed: 31912925
- Cheong A et al. Nuclear-encoded mitochondrial ribosomal proteins are required to initiate gastrulation. Development 147:N/A (2020). PubMed: 32376682
- Signes A et al. APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS. EMBO Mol Med 11:N/A (2019). PubMed: 30552096
- Mohanraj K et al. Inhibition of proteasome rescues a pathogenic variant of respiratory chain assembly factor COA7. EMBO Mol Med 11:N/A (2019). PubMed: 30885959
- Kim SE et al. Upregulation of cytochrome c oxidase subunit 6b1 (Cox6b1) and formation of mitochondrial supercomplexes: implication of Cox6b1 in the effect of calorie restriction. Age (Dordr) 37:9787 (2015). PubMed: 25929654
