Recombinant Anti-COX6B1 antibody [EPR7647] (ab131277)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX6B1 antibody [EPR7647] (ab131277)](https://www.abcam.com/ps/products/131/ab131277/Images/ab131277-145662-anti-cytochrome-c-oxidase-subunit-vib-antibody-epr7647-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX6B1 antibody [EPR7647] (ab131277)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7647] to COX6B1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-COX6B1 antibody [EPR7647]
See all COX6B1 primary antibodies -
Description
Rabbit monoclonal [EPR7647] to COX6B1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HL60, HeLa, HepG2 and Caco 2 cell lysates; HepG2 cells;. Human colon tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.49 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7647 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
|
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| IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| ICC/IF |
1/50 - 1/100.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/50 - 1/100. |
Target
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Function
Connects the two COX monomers into the physiological dimeric form. -
Involvement in disease
Defects in COX6B1 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome. -
Sequence similarities
Belongs to the cytochrome c oxidase subunit 6B family. -
Cellular localization
Mitochondrion intermembrane space. - Information by UniProt
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Database links
- Entrez Gene: 1340 Human
- Omim: 124089 Human
- SwissProt: P14854 Human
- Unigene: 431668 Human
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Alternative names
- COX 6B antibody
- COX VIb 1 antibody
- COX VIb-1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX6B1 antibody [EPR7647] (ab131277)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling COX6B1 with ab131277 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-COX6B1 antibody [EPR7647] (ab131277)](https://www.abcam.com/ps/products/131/ab131277/Images/ab131277-145663-anti-cytochrome-c-oxidase-subunit-vib-antibody-epr7647-western-blot.jpg)
Western blot - Anti-COX6B1 antibody [EPR7647] (ab131277)All lanes : Anti-COX6B1 antibody [EPR7647] (ab131277) at 1/1000 dilution
Lane 1 : HL60 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 10 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [EPR7647] (ab131277)](https://www.abcam.com/ps/products/131/ab131277/Images/ab131277-145661-anti-cytochrome-c-oxidase-subunit-vib-antibody-epr7647-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [EPR7647] (ab131277)Immunofluorescent analysis of HepG2 cells labelling COX6B1 with ab131277 at 1/50 dilution.
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![OI-RD Scanning - Anti-COX6B1 antibody [EPR7647] (ab131277)](https://www.abcam.com/ps/products/131/ab131277/Images/ab131277-145660-anti-cytochrome-c-oxidase-subunit-vib-antibody-epr7647.jpg)
OI-RD Scanning - Anti-COX6B1 antibody [EPR7647] (ab131277) -
![Anti-COX6B1 antibody [EPR7647] (ab131277)](https://www.abcam.com/ps/products/131/ab131277/Images/ab131277-5-benefits-of-recombinant-antibodies.png)
Anti-COX6B1 antibody [EPR7647] (ab131277)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab131277 has been referenced in 1 publication.
- Fan W et al. Glioma cells are resistant to inflammation-induced alterations of mitochondrial dynamics. Int J Oncol 57:1293-1306 (2020). PubMed: 33174046