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    products/primary-antibodies/cox7b-antibody-2g7h8ad9-ab110269.pdf

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Signal Transduction Metabolism Mitochondrial
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Anti-COX7B antibody [2G7H8AD9] (ab110269)

  • Datasheet
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Western blot - Anti-COX7B antibody [2G7H8AD9] (ab110269)

    Key features and details

    • Mouse monoclonal [2G7H8AD9] to COX7B
    • Suitable for: WB
    • Reacts with: Cow
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-COX7B antibody [2G7H8AD9]
      See all COX7B primary antibodies
    • Description

      Mouse monoclonal [2G7H8AD9] to COX7B
    • Host species

      Mouse
    • Specificity

      ab110269 cross-reacts weakly with Human and Rat.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Cow
    • Immunogen

      Full length protein. This information is considered to be commercially sensitive.

    • Positive control

      • Bovine heart lysate
    • General notes

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

      Product was previously marketed under the MitoSciences sub-brand.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.5
      Preservative: 0.02% Sodium azide
      Constituent: HEPES buffered saline
    • Concentration information loading...
    • Purification notes

      Near homogeneity as judged by SDS-PAGE. ab110269 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation
    • Clonality

      Monoclonal
    • Clone number

      2G7H8AD9
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Signal Transduction
      • Metabolism
      • Mitochondrial
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitochondrial markers
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Oxidative phosphorylation
      • Complex IV
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab110269 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 25 µg/ml. Predicted molecular weight: 9 kDa.
    Notes
    WB
    Use a concentration of 25 µg/ml. Predicted molecular weight: 9 kDa.

    Target

    • Function

      This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
    • Sequence similarities

      Belongs to the cytochrome c oxidase VIIb family.
    • Cellular localization

      Mitochondrion inner membrane.
    • Target information above from: UniProt accession P24311 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • COX7B antibody
      • COX7B_HUMAN antibody
      • Cytochrome c oxidase chain VIIb antibody
      • Cytochrome c oxidase polypeptide VIIb antibody
      • Cytochrome c oxidase subunit 7B antibody
      • Cytochrome c oxidase subunit 7B mitochondrial antibody
      • Cytochrome c oxidase subunit VIIb antibody
      • mitochondrial antibody
      see all

    Images

    • Western blot - Anti-COX7B antibody [2G7H8AD9] (ab110269)
      Western blot - Anti-COX7B antibody [2G7H8AD9] (ab110269)
      Anti-COX7B antibody [2G7H8AD9] (ab110269) at 25 µg/ml + Bovine heart lysate at 1 µg

      Predicted band size: 9 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab110269? Please let us know so that we can cite the reference in this datasheet.

    ab110269 has been referenced in 1 publication.

    • Sohal RS  et al. Age-related decrease in expression of mitochondrial DNA encoded subunits of cytochrome c oxidase in Drosophila melanogaster. Mech Ageing Dev 129:558-61 (2008). PubMed: 18538373

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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