Anti-CPEB3 antibody (ab10883)
Key features and details
- Rabbit polyclonal to CPEB3
- Suitable for: ICC, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CPEB3 antibody -
Description
Rabbit polyclonal to CPEB3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: SKNSH cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use a concentration of 1 µg/ml.
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| WB |
1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| Notes |
|---|
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ICC
Use a concentration of 1 µg/ml. |
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WB
1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). |
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ICC/IF
Use a concentration of 5 µg/ml. |
Target
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Sequence similarities
Belongs to the RRM CPEB family.
Contains 2 RRM (RNA recognition motif) domains. - Information by UniProt
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Database links
- Entrez Gene: 22849 Human
- Entrez Gene: 208922 Mouse
- SwissProt: Q8NE35 Human
- SwissProt: Q7TN99 Mouse
- Unigene: 131683 Human
- Unigene: 701732 Human
- Unigene: 391176 Mouse
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Alternative names
- CPE-binding protein 3 antibody
- CPE-BP3 antibody
- CPEB 3 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CPEB3 antibody (ab10883)
ab10883 staining CPEB3 in SKNSH cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab10883 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-CPEB3 antibody (ab10883)Lane 1 : Anti-CPEB3 antibody (ab10883) at 1/500 dilution
Lane 2 : Anti-CPEB3 antibody (ab10883) at 1/1000 dilution
All lanes : Mouse Brain lysate
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution
Predicted band size: 75 kDa
Exposure time: 3 minutesLanes 1 to 2
Lane 1: 5µ
Lane 2: 5µ
Exposure time: 3 minutes
Expected molecular weight: 78kDa
Secondary ab: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed
ab7090 (1/5000)We think that the top band here is the correct band, as it is the same size as the most intense band on the previous blot. The banding pattern is the same on both blots and the lower bands may be clevage products as there is no evidence of CPEB3 isoforms in the literature.
We think that the top band here is the correct band, as it is the same size as the most intense band on the previous blot. The banding pattern is the same on both blots and the lower bands may be clevage products as there is no evidence of CPEB3 isoforms in the literature.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab10883 has been referenced in 12 publications.
- Shu Z et al. MiR-9-5p promotes cell proliferation and migration of hepatocellular carcinoma by targeting CPEB3. Biomark Med 15:97-108 (2021). PubMed: 33496636
- He Y et al. Long non-coding RNA MIR22HG inhibits glioma progression by downregulating microRNA-9/CPEB3. Oncol Lett 21:157 (2021). PubMed: 33552275
- Pascual R et al. The RNA binding protein CPEB2 regulates hormone sensing in mammary gland development and luminal breast cancer. Sci Adv 6:eaax3868 (2020). PubMed: 32440535
- Joag H et al. A role of cellular translation regulation associated with toxic Huntingtin protein. Cell Mol Life Sci 77:3657-3670 (2020). PubMed: 31796991
- Chen Y et al. Long non-coding RNA HCG11 modulates glioma progression through cooperating with miR-496/CPEB3 axis. Cell Prolif 52:e12615 (2019). PubMed: 31310044