Anti-CPNE1 antibody (ab155675)
Key features and details
- Rabbit polyclonal to CPNE1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CPNE1 antibody
See all CPNE1 primary antibodies -
Description
Rabbit polyclonal to CPNE1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Cow, Dog, Pig
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 250-537 of Human CPNE1 (UniProt ID: Q99829).
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji and Mouse brain whole cell lysates; Human Cal27 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 59 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 59 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May function in membrane trafficking. Exhibits calcium-dependent phospholipid binding properties. -
Sequence similarities
Belongs to the copine family.
Contains 2 C2 domains.
Contains 1 VWFA domain. - Information by UniProt
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Database links
- Entrez Gene: 615677 Cow
- Entrez Gene: 477213 Dog
- Entrez Gene: 8904 Human
- Entrez Gene: 266692 Mouse
- Entrez Gene: 100233174 Pig
- Entrez Gene: 100328904 Rabbit
- Omim: 604205 Human
- SwissProt: Q08DB4 Cow
see all -
Alternative names
- Copine 1 antibody
- Copine I antibody
- Copine-1 antibody
see all
Images
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Western blot - Anti-CPNE1 antibody (ab155675)All lanes : Anti-CPNE1 antibody (ab155675) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 59 kDa
7.5% SDS PAGE -
Western blot - Anti-CPNE1 antibody (ab155675)Anti-CPNE1 antibody (ab155675) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 59 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPNE1 antibody (ab155675)Immunohistochemical analysis of paraffin-embedded Human Cal27 xenograft tissue labeling CPNE1 with ab155675 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab155675 has been referenced in 8 publications.
- Chen L et al. CPNE1 regulates myogenesis through the PERK-eIF2α pathway mediated by endoplasmic reticulum stress. Cell Tissue Res 391:545-560 (2023). PubMed: 36525128
- Wang A et al. CPNE1 promotes non-small cell lung cancer progression by interacting with RACK1 via the MET signaling pathway. Cell Commun Signal 20:16 (2022). PubMed: 35101055
- Su J et al. CPNE1 is a potential prognostic biomarker, associated with immune infiltrates and promotes progression of hepatocellular carcinoma. Cancer Cell Int 22:67 (2022). PubMed: 35139863
- Wang Y et al. CPNE1 Enhances Colorectal Cancer Cell Growth, Glycolysis, and Drug Resistance Through Regulating the AKT-GLUT1/HK2 Pathway. Onco Targets Ther 14:699-710 (2021). PubMed: 33536762
- Yao J et al. Prevalent intron retention fine-tunes gene expression and contributes to cellular senescence. Aging Cell 19:e13276 (2020). PubMed: 33274830
- Tang H et al. CPNE1 is a target of miR-335-5p and plays an important role in the pathogenesis of non-small cell lung cancer. J Exp Clin Cancer Res 37:131 (2018). PubMed: 29970127
- Liu S et al. High expression of Copine 1 promotes cell growth and metastasis in human lung adenocarcinoma. Int J Oncol 53:2369-2378 (2018). PubMed: 30221693
- Jiang Z et al. CPNE1 silencing inhibits the proliferation, invasion and migration of human osteosarcoma cells. Oncol Rep 39:643-650 (2018). PubMed: 29207139