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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CPS1 antibody [EPR7493-3] (ab129076)

Submit a review Q&A (4)References (4)
Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076)
  • Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7493-3] (ab129076)
  • Flow Cytometry (Intracellular) - Anti-CPS1 antibody [EPR7493-3] (ab129076)
  • Anti-CPS1 antibody [EPR7493-3] (ab129076)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7493-3] to CPS1
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free HRP

Overview

  • Product name

    Anti-CPS1 antibody [EPR7493-3]
    See all CPS1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7493-3] to CPS1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human CPS1 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • WB: Human fetal liver, mouse liver and rat liver tissue lysates and HeLa cell lysate. IHC-P: Human liver tissue. Flow Cyt (intra): HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/20.
WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 165 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/20.
WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 165 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.

  • Tissue specificity

    Primarily in the liver and small intestine.

  • Involvement in disease

    Defects in CPS1 are the cause of carbamoyl phosphate synthetase 1 deficiency (CPS1D) [MIM:237300]. CPS1D is an autosomal recessive disorder of the urea cycle causing hyperammonemia. Clinical features include protein intolerance, intermittent ataxia, seizures, lethargy, developmental delay and mental retardation.
    Note=Genetic variations in CPS1 influence the availability of precursors for nitric oxide (NO) synthesis and play a role in clinical situations where endogenous NO production is critically important, such as neonatal pulmonary hypertension, increased pulmonary artery pressure following surgical repair of congenital heart defects or hepatovenocclusive disease following bone marrow transplantation. Infants with neonatal pulmonary hypertension homozygous for Thr-1406 have lower L-arginine concentrations than neonates homozygous for Asn-1406.

  • Sequence similarities

    Contains 2 ATP-grasp domains.
    Contains 1 glutamine amidotransferase type-1 domain.

  • Domain

    The type-1 glutamine amidotransferase domain is defective.

  • Cellular localization

    Mitochondrion.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Carbamoyl phosphate synthase [ammonia] antibody
    • Carbamoyl phosphate synthase [ammonia] mitochondrial antibody
    • Carbamoyl phosphate synthase antibody
    • Carbamoyl phosphate synthetase 1 antibody
    • Carbamoyl phosphate synthetase 1 mitochondrial antibody
    • Carbamoyl phosphate synthetase I antibody
    • Carbamoyl-phosphate synthase [ammonia] antibody
    • Carbamoyl-phosphate synthetase I antibody
    • Carbamoylphosphate synthase antibody
    • Carbamoylphosphate synthetase 1 antibody
    • Carbamoylphosphate synthetase I antibody
    • CPS 1 antibody
    • Cps1 antibody
    • CPSase 1 antibody
    • CPSase I antibody
    • CPSASE1 antibody
    • CPSM_HUMAN antibody
    • mitochondrial antibody
    • MS738 antibody
    see all

Images

  • Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076)
    Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076)
    All lanes : Anti-CPS1 antibody [EPR7493-3] (ab129076) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : CPS1 knockout HeLa cell lysate
    Lane 3 : Human liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 165 kDa
    Observed band size: 165 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab129076 observed at 165 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab129076 Anti-CPS1 antibody [EPR7493-3] was shown to specifically react with CPS1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261809 (knockout cell lysate ab257121) was used. Wild-type and CPS1 knockout samples were subjected to SDS-PAGE. ab129076 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076)
    Western blot - Anti-CPS1 antibody [EPR7493-3] (ab129076)
    All lanes : Anti-CPS1 antibody [EPR7493-3] (ab129076) at 1/1000 dilution

    Lane 1 : Fetal liver cell lysate
    Lane 2 : Mouse liver cell lysate
    Lane 3 : Rat liver cell lysate
    Lane 4 : HeLa cell lysate

    Predicted band size: 165 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7493-3] (ab129076)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7493-3] (ab129076)

    ab129076, at 1/100 dilution staining CPS1 in formalin fixed paraffin embedded Human liver tissue by immunohistochemistry

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-CPS1 antibody [EPR7493-3] (ab129076)
    Flow Cytometry (Intracellular) - Anti-CPS1 antibody [EPR7493-3] (ab129076)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling CPS1 (red) with ab129076 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue). 

     

     

  • Anti-CPS1 antibody [EPR7493-3] (ab129076)
    Anti-CPS1 antibody [EPR7493-3] (ab129076)

References (4)

Publishing research using ab129076? Please let us know so that we can cite the reference in this datasheet.

ab129076 has been referenced in 4 publications.

  • Wang W  et al. Tandem Mass Tag-Based Proteomic Analysis of Potential Biomarkers for Hepatocellular Carcinoma Differentiation. Onco Targets Ther 14:1007-1020 (2021). PubMed: 33603407
  • Daniel P  et al. Differentially Expressed Mitochondrial Proteins in Human MCF7 Breast Cancer Cells Resistant to Paclitaxel. Int J Mol Sci 20:N/A (2019). PubMed: 31248089
  • Lin JR  et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. Elife 7:N/A (2018). PubMed: 29993362
  • Snider NT  et al. Ethanol and Acetaminophen Synergistically Induce Hepatic Aggregation and TCH346-Insensitive Nuclear Translocation of GAPDH. PLoS One 11:e0160982 (2016). Mouse . PubMed: 27513663

Customer reviews and Q&As

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Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Question

I found the epitope sequence for ab128942 from the link you provided. but no info regarding the epitope is provided from the webpage of ab129076. would greatly appreciate if epitope squence(s) can be provided.

Read More
Answer

Thank you for your reply.

The exact immunogen sequence for ab129076 is proprietary, but I can inform you that it will bind between amino *.

Please let me know if there is anything else I can help you with.

Read More

Question

thank you for the info. I am wondering if it is possible to let me know which domain (glutaminase, bicarbonate phosphorylation, carbamate phosphorylation, or NAG-binding) do the epitopes located for both ab129076 and ab128942? I am currently analyzing proteolytic fragments of CPS1.

Read More
Answer

Thank you for your reply.

All of the information regarding the location of the epitopes for ab129076 and ab128942 can be found on the webpage and datasheet for these individual products, links to these are below:

https://www.abcam.com/cps1-antibody-epr7494-ab128942.html
https://www.abcam.com/cps1-antibody-epr7493-ab129076.html



In terms of which domain that epiotpe corresponds to, I would recommend searching the uniprot sequence for CSP1 (http://www.uniprot.org/), as it may be able to supply that information, we do not currently have that in our notes.

If there is anything else I can help you with, please let me know.

Read More

Question

Hi,
Where is the epitope for ab129076 located? Or is the epitope sequence available?
I am looking for antibody against C-terminal of human CPS1. would you let me know any antibody from ABCAM is good for WB CPS1 N-terminal?

Read More
Answer

Thank you for contacting Abcam.

The immunogen sequence for a129076 is closer to the N- terminus rather than the C-terminus and so it would not be suitable for you. However, ab128942, would be suitable for you. The epitope is at the C-terminal region and it will react with human samples:

https://www.abcam.com/CPS1-antibody-EPR7494-ab128942.html.



If there is anything else I can help you with, please let me know.

Read More

Question

Is the concentration of ab129076, lot GR87317-2 available?

Read More
Answer

The concentration for ab129076, lot GR87317-2, is 0.131 mg/mL.
I hope this is helpful. Please contact me again if you have any further questions.

Read More

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