Recombinant Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)
![Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)](https://www.abcam.com/ps/products/234/ab234111/Images/ab234111-449193-anti-cpt1a-antibody-epr21843-71-2f-western-blot-hek-293t-lysates.jpg "Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21843-71-2F] to CPT1A
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CPT1A antibody [EPR21843-71-2F]
See all CPT1A primary antibodies -
Description
Rabbit monoclonal [EPR21843-71-2F] to CPT1A -
Host species
Rabbit -
Specificity
We detected weak cross-reactivity with CPT1B with the recombinant protein only. -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HAP1 whole cell lysate; HEK-293T, HeLa, SK-OV-3, C6 and MCF7 whole cell lysates; Mouse, rat and human kidney lysates. IHC-P: Human kidney, ovarian carcinoma and heart tissues; Mouse and rat kidney tissues. Flow Cyt (intra): SK-OV-3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21843-71-2F -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/50.
|
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| WB |
1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
|
|
| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/50. |
|
WB
1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa). |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Strong expression in kidney and heart, and lower in liver and skeletal muscle. -
Pathway
Lipid metabolism; fatty acid beta-oxidation. -
Involvement in disease
Defects in CPT1A are the cause of carnitine palmitoyltransferase 1A deficiency (CPT1AD) [MIM:255120]; also known as CPT-I deficiency or CPT1A deficiency. CPT1AD is a rare autosomal recessive metabolic disorder of long-chain fatty acid oxidation characterized by severe episodes of hypoketotic hypoglycemia usually occurring after fasting or illness. Onset is in infancy or early childhood. -
Sequence similarities
Belongs to the carnitine/choline acetyltransferase family. -
Cellular localization
Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 1374 Human
- Entrez Gene: 12894 Mouse
- Entrez Gene: 25757 Rat
- Omim: 600528 Human
- SwissProt: P50416 Human
- SwissProt: P97742 Mouse
- SwissProt: P32198 Rat
- Unigene: 503043 Human
see all -
Alternative names
- Carnitine O palmitoyltransferase 1 liver isoform antibody
- Carnitine O palmitoyltransferase I antibody
- Carnitine O palmitoyltransferase I liver isoform antibody
see all
Images
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Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)All lanes : Anti-CPT1A antibody [EPR21843-71-2F] (ab234111) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : CPT1A knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 88 kDaLanes 1- 2: Merged signal (red and green). Green - ab234111 observed at 88 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab234111 was shown to react with CPT1A in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266319 (knockout cell lysate ab256880) was used. Wild-type HEK-293T and CPT1A knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab234111 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)](https://www.abcam.com/ps/products/234/ab234111/Images/ab234111-311116-anti-cpt1a-antibody-epr21843-71-2f-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CPT1A with ab234111 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining in human kidney (PMID: 18192268; PMID: 28956034; PMID: 15363638; PMID: 8679700) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)](https://www.abcam.com/ps/products/234/ab234111/Images/ab234111-311114-anti-cpt1a-antibody-epr21843-71-2f-western-blot.jpg)
Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)All lanes : Anti-CPT1A antibody [EPR21843-71-2F] (ab234111) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor cell line) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Rat kidney lysate
Lane 5 : Human kidney lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 7 : SK-OV-3 (human ovarian cancer cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-4 & 6-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 88 kDa
Observed band size: 88 kDaExposure time : Lanes 1 and 2: 3 minutes; Lane 3: 37 seconds; Lane 4: 114 seconds; Lane 5: 15 seconds; Lanes 6 and 7: 92 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)](https://www.abcam.com/ps/products/234/ab234111/Images/ab234111-311119-anti-cpt1a-antibody-epr21843-71-2f-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling CPT1A with ab234111 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining in rat kidney (PMID: 18192268; PMID: 28956034; PMID: 15363638; PMID: 8679700) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)](https://www.abcam.com/ps/products/234/ab234111/Images/ab234111-311118-anti-cpt1a-antibody-epr21843-71-2f-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling CPT1A with ab234111 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in mouse kidney (PMID: 18192268; PMID: 28956034; PMID: 15363638; PMID: 8679700) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)](https://www.abcam.com/ps/products/234/ab234111/Images/ab234111-311117-anti-cpt1a-antibody-epr21843-71-2f-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue labeling CPT1A with ab234111 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining in human ovarian carcinoma (PMID: 26716645) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Flow Cytometry (Intracellular) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)](https://www.abcam.com/ps/products/234/ab234111/Images/ab234111-311115-anti-cpt1a-antibody-epr21843-71-2f-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized SK-OV-3 (human ovarian cancer cell line) cell line labeling CPT1A with ab234111 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)](https://www.abcam.com/ps/products/234/ab234111/Images/ab234111-311113-anti-cpt1a-antibody-epr21843-71-2f-western-blot.jpg)
Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)All lanes : Anti-CPT1A antibody [EPR21843-71-2F] (ab234111) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CPT1A knockout HAP1 whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : SK-OV-3 (human ovarian cancer cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 88 kDa
Observed band size: 88 kDa
Exposure time: 92 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
ab234111 was shown to specifically react with CPT1A in wild-type HAP1 cells as signal was lost in CPT1A knockout cells. Wild-type and CPT1A knockout samples were subjected to SDS-PAGE. ab234111 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)](https://www.abcam.com/ps/products/234/ab234111/Images/ab234111-311125-anti-cpt1a-antibody-epr21843-71-2f-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)Immunohistochemical analysis of paraffin-embedded human heart tissue labeling CPT1A with ab234111 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human heart is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)](https://www.abcam.com/ps/products/234/ab234111/Images/ab234111-10-benefits-of-recombinant-antibodies.png)
Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (10)
ab234111 has been referenced in 10 publications.
- Prieto-Carrasco R et al. Progressive Reduction in Mitochondrial Mass Is Triggered by Alterations in Mitochondrial Biogenesis and Dynamics in Chronic Kidney Disease Induced by 5/6 Nephrectomy. Biology (Basel) 10:N/A (2021). PubMed: 33919054
- Luo L et al. Influence of atorvastatin on metabolic pattern of rats with pulmonary hypertension. Aging (Albany NY) 13:11954-11968 (2021). PubMed: 33886502
- Guo W et al. Microalgae polysaccharides ameliorates obesity in association with modulation of lipid metabolism and gut microbiota in high-fat-diet fed C57BL/6 mice. Int J Biol Macromol 182:1371-1383 (2021). PubMed: 34004199
- Luo Z et al. Astragaloside IV ameliorates fat metabolism in the liver of ageing mice through targeting mitochondrial activity. J Cell Mol Med 25:8863-8876 (2021). PubMed: 34402182
- Zhu X et al. ANGPTL4 Attenuates Ang II-Induced Atrial Fibrillation and Fibrosis in Mice via PPAR Pathway. Cardiol Res Pract 2021:9935310 (2021). PubMed: 34422410
- Yuan S et al. Amelioration of the Lipogenesis, Oxidative Stress and Apoptosis of Hepatocytes by a Novel Proteoglycan from Ganoderma lucidum. Biol Pharm Bull 43:1542-1550 (2020). PubMed: 32759548
- Wei X et al. Reducing NADPH Synthesis Counteracts Diabetic Nephropathy through Restoration of AMPK Activity in Type 1 Diabetic Rats. Cell Rep 32:108207 (2020). PubMed: 32997989
- Lovisa S et al. Endothelial-to-mesenchymal transition compromises vascular integrity to induce Myc-mediated metabolic reprogramming in kidney fibrosis. Sci Signal 13:N/A (2020). PubMed: 32518142
- Yang S et al. Hypoglycemic effects of polysaccharides from Gomphidiaceae rutilus fruiting bodies and their mechanisms. Food Funct 11:424-434 (2020). PubMed: 31828269
- Zhang S et al. Efferocytosis Fuels Requirements of Fatty Acid Oxidation and the Electron Transport Chain to Polarize Macrophages for Tissue Repair. Cell Metab 29:443-456.e5 (2019). PubMed: 30595481