Anti-CPT2 antibody [1C2AE6] (ab110293)
![Immunocytochemistry/ Immunofluorescence - Anti-CPT2 antibody [1C2AE6] (ab110293)](https://www.abcam.com/ps/products/110/ab110293/Images/ab110293-123528-anti-cpt2-antibody-1c2ae6-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CPT2 antibody [1C2AE6] (ab110293)")
Key features and details
- Mouse monoclonal [1C2AE6] to CPT2
- Suitable for: IHC-P, ICC/IF, Flow Cyt, IP
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
Overview
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Product name
Anti-CPT2 antibody [1C2AE6]
See all CPT2 primary antibodies -
Description
Mouse monoclonal [1C2AE6] to CPT2 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cyt, IPmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
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Positive control
- HepG2 cells. HL-60 cells. Human normal colon FFPE tissue
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
1C2AE6 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 10 µg/ml.
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| ICC/IF |
Use a concentration of 4 µg/ml.
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| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IP |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-P
Use a concentration of 10 µg/ml. |
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ICC/IF
Use a concentration of 4 µg/ml. |
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Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IP
Use at an assay dependent concentration. |
Target
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Pathway
Lipid metabolism; fatty acid beta-oxidation. -
Involvement in disease
Defects in CPT2 are the cause of carnitine palmitoyltransferase 2 deficiency (CPT2D) [MIM:255110, 600649]; also known as CPT-II deficiency or CPT2 deficiency. CPT2D is an autosomal recessive disorder characterized by recurrent myoglobinuria, episodes of muscle pain, stiffness, and rhabdomyolysis. These symptoms are triggered by prolonged exercise, fasting or viral infection and patients are usually young adults. In addition to this classical, late-onset, muscular type, a hepatic or hepatocardiomuscular form has been reported in infants. Clinical pictures in these children or neonates include hypoketotic hypoglycemia, liver dysfunction, cardiomyopathy and sudden death.
Defects in CPT2 are the cause of carnitine palmitoyltransferase 2 deficiency, lethal neonatal (CPT2D-LN) [MIM:608836]; also known as lethal neonatal CPT-II deficiency. It is a lethal neonatal form of CPT2D. This rarely presentation is antenatal with cerebral periventricular cysts and cystic dysplastic kidneys. The clinical variability of the disease is likely attributed to the variable residual enzymatic activity. -
Sequence similarities
Belongs to the carnitine/choline acetyltransferase family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 504502 Cow
- Entrez Gene: 1376 Human
- Entrez Gene: 12896 Mouse
- Entrez Gene: 25413 Rat
- Omim: 600650 Human
- SwissProt: Q2KJB7 Cow
- SwissProt: P23786 Human
- SwissProt: P52825 Mouse
see all -
Alternative names
- Carnitine O palmitoyltransferase 2 antibody
- Carnitine O palmitoyltransferase 2 mitochondrial antibody
- Carnitine O-palmitoyltransferase 2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CPT2 antibody [1C2AE6] (ab110293)Immunocytochemistry image of ab110293 stained Human HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab110293 at 4 µg/ml for 2h at room temperature, or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
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![Flow Cytometry - Anti-CPT2 antibody [1C2AE6] (ab110293)](https://www.abcam.com/ps/products/110/ab110293/Images/ab110293-123527-anti-cpt2-antibody-1c2ae6-flow-cytometry.jpg)
Flow Cytometry - Anti-CPT2 antibody [1C2AE6] (ab110293)HL-60 cells were stained with 1 µg/mL of ab110293 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry. -
![Immunoprecipitation - Anti-CPT2 antibody [1C2AE6] (ab110293)](https://www.abcam.com/ps/products/110/ab110293/Images/ab110293-123526-anti-cpt2-antibody-1c2ae6-immunoprecipitation.gif)
Immunoprecipitation - Anti-CPT2 antibody [1C2AE6] (ab110293)SDS-PAGE analysis of IP samples. As an IP antibody, ab110293 works with human, bovine, rat, and mouse samples.
Lane STD: Molecular weight ladder
Lane HLM: Human liver mitochondria
Lane BHM: Bovine heart mitochondria
Lane RLM: Rat liver mitochondria
Lane MLM: Mouse liver mitochondria
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [1C2AE6] (ab110293)](https://www.abcam.com/ps/products/110/ab110293/Images/ab110293-123525-anti-cpt2-antibody-1c2ae6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [1C2AE6] (ab110293)IHC image of CPT2 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110293, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab110293 has been referenced in 4 publications.
- Liu L et al. Low-Level Saturated Fatty Acid Palmitate Benefits Liver Cells by Boosting Mitochondrial Metabolism via CDK1-SIRT3-CPT2 Cascade. Dev Cell 52:196-209.e9 (2020). PubMed: 31866205
- Zhang L et al. The Protective Activities of Dietary Sea Cucumber Cerebrosides against Atherosclerosis through Regulating Inflammation and Cholesterol Metabolism in Male Mice. Mol Nutr Food Res 62:e1800315 (2018). PubMed: 29883529
- Jernberg JN et al. Developmental regulation and localization of carnitine palmitoyltransferases (CPTs) in rat brain. J Neurochem 142:407-419 (2017). PubMed: 28512781
- Townsend KL et al. Increased Mitochondrial Activity in BMP7-Treated Brown Adipocytes, Due to Increased CPT1- and CD36-Mediated Fatty Acid Uptake. Antioxid Redox Signal : (2012). PubMed: 22938691