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RecombinantRabMAb

Recombinant Anti-CRABP1 antibody [EPR7905] (ab182573)

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Western blot - Anti-CRABP1 antibody [EPR7905] (ab182573)
  • Immunoprecipitation - Anti-CRABP1 antibody [EPR7905] (ab182573)
  • Western blot - Anti-CRABP1 antibody [EPR7905] (ab182573)
  • Anti-CRABP1 antibody [EPR7905] (ab182573)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7905] to CRABP1
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-CRABP1 antibody [EPR7905]
    See all CRABP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7905] to CRABP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Y79, MCF7 and Human skin lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab182573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/2000. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa).
IP
1/50 - 1/70.
Notes
WB
1/1000 - 1/2000. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa).
IP
1/50 - 1/70.

Target

  • Function

    Cytosolic CRABPs may regulate the access of retinoic acid to the nuclear retinoic acid receptors.

  • Sequence similarities

    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

  • Domain

    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cellular retinoic acid binding protein 1 antibody
      • Cellular retinoic acid-binding protein 1 antibody
      • Cellular retinoic acid-binding protein I antibody
      • CRABP 1 antibody
      • CRABP antibody
      • CRABP I antibody
      • CRABP-I antibody
      • Crabp1 antibody
      • CRABPI antibody
      • RABP1_HUMAN antibody
      • RBP 5 antibody
      • RBP5 antibody
      • Retinoic acid binding protein I cellular antibody
      see all

    Images

    • Western blot - Anti-CRABP1 antibody [EPR7905] (ab182573)
      Western blot - Anti-CRABP1 antibody [EPR7905] (ab182573)
      Anti-CRABP1 antibody [EPR7905] (ab182573) at 1/1000 dilution + Human skin lysate at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 16 kDa

    • Immunoprecipitation - Anti-CRABP1 antibody [EPR7905] (ab182573)
      Immunoprecipitation - Anti-CRABP1 antibody [EPR7905] (ab182573)

      Western blot analysis of CRABP1 in immunoprecipitation pellets from Human Y79 lysate. ab182573 used at 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.

    • Western blot - Anti-CRABP1 antibody [EPR7905] (ab182573)
      Western blot - Anti-CRABP1 antibody [EPR7905] (ab182573)
      All lanes : Anti-CRABP1 antibody [EPR7905] (ab182573) at 1/1000 dilution

      Lane 1 : Y79 cell lysate
      Lane 2 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 16 kDa

    • Anti-CRABP1 antibody [EPR7905] (ab182573)
      Anti-CRABP1 antibody [EPR7905] (ab182573)

    References (0)

    Publishing research using ab182573? Please let us know so that we can cite the reference in this datasheet.

    ab182573 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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