Recombinant Anti-CRABP2 antibody [EPR14256(B)] (ab181255)

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Western blot - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14256(B)] to CRABP2
Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
Reacts with: Human

Carrier Free

Product name

Anti-CRABP2 antibody [EPR14256(B)]
See all CRABP2 primary antibodies
Description

Rabbit monoclonal [EPR14256(B)] to CRABP2
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- MCF7 cell lysate; Human skin lysate; Human liver and kidney lysates; MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14256(B)
Isotype

IgG
Research areas

Alternative Versions
- Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human CRABP2 protein (ab74527)
Related Products
- Recombinant Human CRABP2 protein (ab74527)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/80. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB		1/10000 - 1/50000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
ICC/IF		1/100 - 1/250.

Notes
Flow Cyt (Intra) 1/80. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/50000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
ICC/IF 1/100 - 1/250.

Function

Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors.
Sequence similarities

Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
Domain

Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
Cellular localization

Cytoplasm. Nucleus. Upon ligand binding, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus.
Target information above from: UniProt accession P29373 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 1382 Human
- Omim: 180231 Human
- SwissProt: P29373 Human
- Unigene: 405662 Human
Alternative names
- Cellular retinoic acid binding protein 2 antibody
- Cellular retinoic acid binding protein II antibody
- Cellular retinoic acid-binding protein 2 antibody
- Cellular retinoic acid-binding protein II antibody
- CRABP II antibody
- CRABP-II antibody
- Crabp2 antibody
- CRABPII antibody
- RABP2_HUMAN antibody
- RBP6 antibody
- Rretinoic acid-binding protein, cellular, type II antibody
see all

Western blot - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)

Anti-CRABP2 antibody [EPR14256(B)] (ab181255) at 1/50000 dilution + MCF7 cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 16 kDa
Observed band size: 16 kDa
Western blot - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)

Anti-CRABP2 antibody [EPR14256(B)] (ab181255) at 1/10000 dilution + Human skin cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

Developed using the ECL technique.

Predicted band size: 16 kDa
Observed band size: 16 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)

Immunohistochemical analysis of paraffin-embedded, Human liver tissue labeling CRABP2 with ab181255 at a 1/100 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)

Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling CRABP2 with ab181255 at a 1/100 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)

Immunofluorescence analysis of, paraformaldehyde-fixed, MCF7 cells labeling CRABP2 with ab181255 at a 1/250 dilution. As secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) (green) was used at a 1/200 dilution. DAPI staining in blue.
Flow Cytometry (Intracellular) - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)

Intracellular Flow Cytometry analysis of, paraformaldehyde-fixed, MCF7 cells labeling CRABP2 (red) with ab181255 at a 1/80 dilution, or negative control rabbit monoclonal IgG (green). As secondary antibody goat anti rabbit IgG (FITC) was used at a 1/150 dilution.
Anti-CRABP2 antibody [EPR14256(B)] (ab181255)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab181255? Please let us know so that we can cite the reference in this datasheet.

ab181255 has been referenced in 4 publications.

Wu Y et al. Circular RNA hsa_circ_0011298 enhances Taxol resistance of non-small cell lung cancer by regulating miR-486-3p/CRABP2 axis. J Clin Lab Anal 36:e24408 (2022). PubMed: 35396749
Wang Y et al. Single-cell analysis of pancreatic ductal adenocarcinoma identifies a novel fibroblast subtype associated with poor prognosis but better immunotherapy response. Cell Discov 7:36 (2021). PubMed: 34035226
Fischer-Huchzermeyer S et al. MEK inhibitors enhance therapeutic response towards ATRA in NF1 associated malignant peripheral nerve sheath tumors (MPNST) in-vitro. PLoS One 12:e0187700 (2017). PubMed: 29131833
Xu Z et al. Wnt/ß-catenin signaling promotes self-renewal and inhibits the primed state transition in naïve human embryonic stem cells. Proc Natl Acad Sci U S A 113:E6382-E6390 (2016). PubMed: 27698112

Customer reviews and Q&As

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Domain

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (4)

Customer reviews and Q&As