Recombinant Anti-CRABP2 antibody [EPR14256(B)] (ab181255)
![Western blot - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)](https://www.abcam.com/ps/products/181/ab181255/Images/ab181255-214521-anti-crabp2-antibody-epr14256b-western-blot.jpg "Western blot - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14256(B)] to CRABP2
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CRABP2 antibody [EPR14256(B)]
See all CRABP2 primary antibodies -
Description
Rabbit monoclonal [EPR14256(B)] to CRABP2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MCF7 cell lysate; Human skin lysate; Human liver and kidney lysates; MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14256(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/80.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| WB |
1/10000 - 1/50000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
|
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| ICC/IF |
1/100 - 1/250.
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| Notes |
|---|
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Flow Cyt (Intra)
1/80. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). |
|
ICC/IF
1/100 - 1/250. |
Target
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Function
Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
Cellular localization
Cytoplasm. Nucleus. Upon ligand binding, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1382 Human
- Omim: 180231 Human
- SwissProt: P29373 Human
- Unigene: 405662 Human
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Alternative names
- Cellular retinoic acid binding protein 2 antibody
- Cellular retinoic acid binding protein II antibody
- Cellular retinoic acid-binding protein 2 antibody
see all
Images
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Western blot - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)Anti-CRABP2 antibody [EPR14256(B)] (ab181255) at 1/50000 dilution + MCF7 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 16 kDa
Observed band size: 16 kDa -
![Western blot - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)](https://www.abcam.com/ps/products/181/ab181255/Images/ab181255-214522-anti-crabp2-antibody-epr14256b-western-blot.jpg)
Western blot - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)Anti-CRABP2 antibody [EPR14256(B)] (ab181255) at 1/10000 dilution + Human skin cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 16 kDa
Observed band size: 16 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)](https://www.abcam.com/ps/products/181/ab181255/Images/ab181255-214524-anti-crabp2-antibody-epr14256b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)Immunohistochemical analysis of paraffin-embedded, Human liver tissue labeling CRABP2 with ab181255 at a 1/100 dilution. Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)](https://www.abcam.com/ps/products/181/ab181255/Images/ab181255-214525-anti-crabp2-antibody-epr14256b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling CRABP2 with ab181255 at a 1/100 dilution. Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)](https://www.abcam.com/ps/products/181/ab181255/Images/ab181255-214526-anti-crabp2-antibody-epr14256b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)Immunofluorescence analysis of, paraformaldehyde-fixed, MCF7 cells labeling CRABP2 with ab181255 at a 1/250 dilution. As secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) (green) was used at a 1/200 dilution. DAPI staining in blue.
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![Flow Cytometry (Intracellular) - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)](https://www.abcam.com/ps/products/181/ab181255/Images/ab181255-214527-anti-crabp2-antibody-epr14256b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)Intracellular Flow Cytometry analysis of, paraformaldehyde-fixed, MCF7 cells labeling CRABP2 (red) with ab181255 at a 1/80 dilution, or negative control rabbit monoclonal IgG (green). As secondary antibody goat anti rabbit IgG (FITC) was used at a 1/150 dilution.
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![Anti-CRABP2 antibody [EPR14256(B)] (ab181255)](https://www.abcam.com/ps/products/181/ab181255/Images/ab181255-7-benefits-of-recombinant-antibodies.png)
Anti-CRABP2 antibody [EPR14256(B)] (ab181255)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab181255 has been referenced in 3 publications.
- Wang Y et al. Single-cell analysis of pancreatic ductal adenocarcinoma identifies a novel fibroblast subtype associated with poor prognosis but better immunotherapy response. Cell Discov 7:36 (2021). PubMed: 34035226
- Fischer-Huchzermeyer S et al. MEK inhibitors enhance therapeutic response towards ATRA in NF1 associated malignant peripheral nerve sheath tumors (MPNST) in-vitro. PLoS One 12:e0187700 (2017). PubMed: 29131833
- Xu Z et al. Wnt/ß-catenin signaling promotes self-renewal and inhibits the primed state transition in naïve human embryonic stem cells. Proc Natl Acad Sci U S A 113:E6382-E6390 (2016). PubMed: 27698112