Name: Anti-CRABP2 antibody [EPR17376]
Brand: RabMAb
SKU: ab211927
Rating: 4 (1 reviews)

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Western blot - Anti-CRABP2 antibody [EPR17376] (ab211927)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17376] to CRABP2
Suitable for: ICC/IF, IHC-P, WB, Flow Cyt (Intra)
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-CRABP2 antibody [EPR17376]
See all CRABP2 primary antibodies
Description

Rabbit monoclonal [EPR17376] to CRABP2
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, IHC-P, WB, Flow Cyt (Intra)more details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human, mouse and rat skin lysates; HT-29 and MCF7 whole cell lysates. IHC-P: Human oesophagus, skin and pancreatic ductal adenocarcinoma tissues; mouse and rat skin tissues. ICC/IF: MCF7 and HT-29 cells. Flow Cyt (intra): MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17376
Isotype

IgG
Research areas

Alternative Versions
- Anti-CRABP2 antibody [EPR17376] - BSA and Azide free (ab223551)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human CRABP2 protein (ab74527)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab211927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF		1/250.
IHC-P	(1)	1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 16 kDa).
Flow Cyt (Intra)		1/600. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
ICC/IF 1/250.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 16 kDa).
Flow Cyt (Intra) 1/600. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Function

Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors.
Sequence similarities

Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
Domain

Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
Cellular localization

Cytoplasm. Nucleus. Upon ligand binding, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus.
Target information above from: UniProt accession P29373 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 1382 Human
- Entrez Gene: 12904 Mouse
- Entrez Gene: 29563 Rat
- Omim: 180231 Human
- SwissProt: P29373 Human
- SwissProt: P22935 Mouse
- SwissProt: P51673 Rat
- Unigene: 405662 Human
- Unigene: 4757 Mouse
- Unigene: 11333 Rat
see all
Alternative names
- Cellular retinoic acid binding protein 2 antibody
- Cellular retinoic acid binding protein II antibody
- Cellular retinoic acid-binding protein 2 antibody
- Cellular retinoic acid-binding protein II antibody
- CRABP II antibody
- CRABP-II antibody
- Crabp2 antibody
- CRABPII antibody
- RABP2_HUMAN antibody
- RBP6 antibody
- Rretinoic acid-binding protein, cellular, type II antibody
see all

Western blot - Anti-CRABP2 antibody [EPR17376] (ab211927)

Lanes 1 & 3 : Anti-CRABP2 antibody [EPR17376] (ab211927) at 1/5000 dilution
Lane 2 : Anti-CRABP2 antibody [EPR17376] (ab211927) at 1/1000 dilution

Lane 1 : Human skin lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 16 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-CRABP2 antibody [EPR17376] (ab211927)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling CRABP2 with ab211927 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on MCF7 cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows:
-ve control 1: ab211927 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed, by followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR17376] (ab211927)

Immunohistochemical analysis of paraffin-embedded human oesophagus tissue labeling CRABP2 with ab211927 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on the stratified squamous epithelium of the human oesophagus is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CRABP2 antibody [EPR17376] (ab211927)

All lanes : Anti-CRABP2 antibody [EPR17376] (ab211927) at 1/1000 dilution

Lane 1 : Mouse skin lysate
Lane 2 : Rat skin lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 16 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR17376] (ab211927)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling CRABP2 with ab211927 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on the stratified squamous epithelium and hair follicle cells of the human skin is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR17376] (ab211927)

Immunohistochemical analysis of paraffin-embedded human pancreatic ductal adenocarcinoma tissue labeling CRABP2 with ab211927 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on the human pancreatic ductal adenocarcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR17376] (ab211927)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling CRABP2 with ab211927 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on the stratified squamous epithelium, hair follicle cells and sweat gland cells of the mouse skin is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR17376] (ab211927)

Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling CRABP2 with ab211927 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on the stratified squamous epithelium and sweat gland cells of the rat skin is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-CRABP2 antibody [EPR17376] (ab211927)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cell line) cells labeling CRABP2 with ab211927 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on HT-29 cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows:
-ve control 1: ab211927 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-CRABP2 antibody [EPR17376] (ab211927)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling CRABP2 with ab211927 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Anti-CRABP2 antibody [EPR17376] (ab211927)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

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Publishing research using ab211927? Please let us know so that we can cite the reference in this datasheet.

ab211927 has been referenced in 4 publications.

Yi Q et al. MiR-579 Inhibits Lung Adenocarcinoma Cell Proliferation and Metastasis via Binding to CRABP2. Comput Math Methods Med 2022:9111681 (2022). PubMed: 35966249
Zhao H et al. LINC01816 promotes the migration, invasion and epithelial-mesenchymal transition of thyroid carcinoma cells by sponging miR-34c-5p and regulating CRABP2 expression levels. Oncol Rep 45:N/A (2021). PubMed: 33786631
Li M et al. Expression and function analysis of CRABP2 and FABP5, and their ratio in esophageal squamous cell carcinoma. Open Med (Wars) 16:1444-1458 (2021). PubMed: 34632074
Zhao X & Yang X Retinoic Acid Promotes Retinoic Acid Signaling by Suppression of Pitx1 In Tendon Cells: A Possible Mechanism of a Clubfoot-Like Phenotype Induced by Retinoic Acid. Med Sci Monit 25:6980-6989 (2019). PubMed: 31527569

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Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Skin explants)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate

Permeabilization

Specification

Skin explants

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Submitted Jul 19 2023

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Domain

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (4)

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CRABP2 antibody [EPR17376]