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RecombinantRabMAb

Recombinant Anti-CRCP antibody [EPR9670(B)] (ab139264)

Submit a review Submit a question References (2)
Western blot - Anti-CRCP antibody [EPR9670(B)] (ab139264)
  • Western blot - Anti-CRCP antibody [EPR9670(B)] (ab139264)
  • Western blot - Anti-CRCP antibody [EPR9670(B)] (ab139264)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] (ab139264)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] (ab139264)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] (ab139264)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] (ab139264)
  • Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] (ab139264)
  • Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] (ab139264)
  • Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] (ab139264)
  • Immunoprecipitation - Anti-CRCP antibody [EPR9670(B)] (ab139264)
  • Immunoprecipitation - Anti-CRCP antibody [EPR9670(B)] (ab139264)
  • Anti-CRCP antibody [EPR9670(B)] (ab139264)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9670(B)] to CRCP
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-CRCP antibody [EPR9670(B)]
    See all CRCP primary antibodies

  • Description

    Rabbit monoclonal [EPR9670(B)] to CRCP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human CRCP aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O75575

  • Positive control

    • WB: HeLa and Jurkat whole cell lysate (ab7899). Human prostate and ovary cancer tissue lysates. Mouse kidney and rat brain tissue lysates. IHC-P: Human ovarian carcinoma and breast tissues. ICC/IF: LnCap and HeLa cells. IP: Human prostate cancer tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab139264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100.
ICC/IF
1/250.

For unpurified use at 1/100.
IP
1/50 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100.
ICC/IF
1/250.

For unpurified use at 1/100.
IP
1/50 - 1/100.

Target

  • Function

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway.
    Accessory protein for the calcitonin gene-related peptide (CGRP) receptor. It modulates CGRP responsiveness in a variety of tissues.

  • Tissue specificity

    Ubiquitous. Most prevalent in testis.

  • Sequence similarities

    Belongs to the eukaryotic RPC9 RNA polymerase subunit family.

  • Cellular localization

    Nucleus. Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Calcitonin gene related peptide receptor component antibody
    • Calcitonin gene related peptide receptor component protein antibody
    • Calcitonin gene-related peptide-receptor component protein antibody
    • CGRP receptor component protein antibody
    • CGRP-RCP antibody
    • CGRP-receptor component protein antibody
    • CGRPRCP antibody
    • CRCP antibody
    • DNA-directed RNA polymerase III subunit RPC9 antibody
    • HsC17 antibody
    • RCP antibody
    • RCP9 antibody
    • RNA polymerase III subunit C9 antibody
    • RPC9_HUMAN antibody
    see all

Images

  • Western blot - Anti-CRCP antibody [EPR9670(B)] (ab139264)
    Western blot - Anti-CRCP antibody [EPR9670(B)] (ab139264)
    All lanes : Anti-CRCP antibody [EPR9670(B)] (ab139264) at 1/5000 dilution (unpurified)

    Lane 1 : Jurkat cell lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 14 kDa
    Observed band size: 20 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-CRCP antibody [EPR9670(B)] (ab139264)
    Western blot - Anti-CRCP antibody [EPR9670(B)] (ab139264)
    All lanes : Anti-CRCP antibody [EPR9670(B)] (ab139264) at 1/10000 dilution (purified)

    Lane 1 : Jurkat cell lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 14 kDa
    Observed band size: 20 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-CRCP antibody [EPR9670(B)] (ab139264)
    Western blot - Anti-CRCP antibody [EPR9670(B)] (ab139264)
    All lanes : Anti-CRCP antibody [EPR9670(B)] (ab139264) at 1/1000 dilution (unpurified)

    Lane 1 : Human ovary cancer lysate
    Lane 2 : HeLa lysate
    Lane 3 : Jurkat lysate
    Lane 4 : Human prostate cancer lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 14 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] (ab139264)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] (ab139264)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with unpurified ab139264 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] (ab139264)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] (ab139264)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with purified ab139264 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] (ab139264)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] (ab139264)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with unpurified ab139264 at 1/50.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] (ab139264)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] (ab139264)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CRCP with unpurified ab139264 at 1/50.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] (ab139264)
    Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] (ab139264)

    Immunocytochemsitry/Immunofluorescence analysis of LnCap cells labelling CRCP (red) with unpurified ab139264 at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] (ab139264)
    Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] (ab139264)

    Immunocytochemsitry/Immunofluorescence analysis of LnCap cells labelling CRCP (red) with purified ab139264 at 1/250. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] (ab139264)
    Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] (ab139264)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CRCP with unpurified ab139264 at 1/100.

  • Immunoprecipitation - Anti-CRCP antibody [EPR9670(B)] (ab139264)
    Immunoprecipitation - Anti-CRCP antibody [EPR9670(B)] (ab139264)

    ab139264 (unpurified) at 1/50 immunoprecipitating CRCP in human prostate cancer tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunoprecipitation - Anti-CRCP antibody [EPR9670(B)] (ab139264)
    Immunoprecipitation - Anti-CRCP antibody [EPR9670(B)] (ab139264)

    ab139264 (purified) at 1/100 immunoprecipitating CRCP in human prostate cancer tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-CRCP antibody [EPR9670(B)] (ab139264)
    Anti-CRCP antibody [EPR9670(B)] (ab139264)

References (2)

Publishing research using ab139264? Please let us know so that we can cite the reference in this datasheet.

ab139264 has been referenced in 2 publications.

  • Mounir MMF  et al. Regeneration of Neural Networks in Immature Teeth with Non-Vital Pulp Following a Novel Regenerative Procedure. Int J Stem Cells 12:410-418 (2019). PubMed: 31658509
  • Bao Y  et al. Topical treatment with Xiaozheng Zhitong Paste alleviates bone cancer pain by inhibiting proteinase-activated receptor 2 signaling pathway. Oncol Rep 34:1449-59 (2015). PubMed: 26133236

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