Recombinant Anti-CRCP antibody [EPR9670(B)] (ab139264)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9670(B)] to CRCP
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CRCP antibody [EPR9670(B)]
See all CRCP primary antibodies -
Description
Rabbit monoclonal [EPR9670(B)] to CRCP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human CRCP aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: O75575 -
Positive control
- WB: HeLa and Jurkat whole cell lysate (ab7899). Human prostate and ovary cancer tissue lysates. Mouse kidney and rat brain tissue lysates. IHC-P: Human ovarian carcinoma and breast tissues. ICC/IF: LnCap and HeLa cells. IP: Human prostate cancer tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9670(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab139264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
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IHC-P |
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100. |
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ICC/IF |
1/250.
For unpurified use at 1/100. |
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IP |
1/50 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa. |
IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100. |
ICC/IF
1/250. For unpurified use at 1/100. |
IP
1/50 - 1/100. |
Target
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Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Accessory protein for the calcitonin gene-related peptide (CGRP) receptor. It modulates CGRP responsiveness in a variety of tissues. -
Tissue specificity
Ubiquitous. Most prevalent in testis. -
Sequence similarities
Belongs to the eukaryotic RPC9 RNA polymerase subunit family. -
Cellular localization
Nucleus. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 27297 Human
- Entrez Gene: 12909 Mouse
- Entrez Gene: 114205 Rat
- Omim: 606121 Human
- SwissProt: O75575 Human
- SwissProt: O35427 Mouse
- SwissProt: Q8VHM6 Rat
- Unigene: 300684 Human
see all -
Alternative names
- Calcitonin gene related peptide receptor component antibody
- Calcitonin gene related peptide receptor component protein antibody
- Calcitonin gene-related peptide-receptor component protein antibody
see all
Images
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All lanes : Anti-CRCP antibody [EPR9670(B)] (ab139264) at 1/5000 dilution (unpurified)
Lane 1 : Jurkat cell lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-CRCP antibody [EPR9670(B)] (ab139264) at 1/10000 dilution (purified)
Lane 1 : Jurkat cell lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-CRCP antibody [EPR9670(B)] (ab139264) at 1/1000 dilution (unpurified)
Lane 1 : Human ovary cancer lysate
Lane 2 : HeLa lysate
Lane 3 : Jurkat lysate
Lane 4 : Human prostate cancer lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 14 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] (ab139264)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with unpurified ab139264 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] (ab139264)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with purified ab139264 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] (ab139264)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with unpurified ab139264 at 1/50.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] (ab139264)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CRCP with unpurified ab139264 at 1/50.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunocytochemsitry/Immunofluorescence analysis of LnCap cells labelling CRCP (red) with unpurified ab139264 at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
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Immunocytochemsitry/Immunofluorescence analysis of LnCap cells labelling CRCP (red) with purified ab139264 at 1/250. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CRCP with unpurified ab139264 at 1/100.
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ab139264 (unpurified) at 1/50 immunoprecipitating CRCP in human prostate cancer tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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ab139264 (purified) at 1/100 immunoprecipitating CRCP in human prostate cancer tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab139264 has been referenced in 2 publications.
- Mounir MMF et al. Regeneration of Neural Networks in Immature Teeth with Non-Vital Pulp Following a Novel Regenerative Procedure. Int J Stem Cells 12:410-418 (2019). PubMed: 31658509
- Bao Y et al. Topical treatment with Xiaozheng Zhitong Paste alleviates bone cancer pain by inhibiting proteinase-activated receptor 2 signaling pathway. Oncol Rep 34:1449-59 (2015). PubMed: 26133236