Anti-Creatine kinase B type antibody [ZN-1] (ab170175)
![Western blot - Anti-Creatine kinase B type antibody [ZN-1] (ab170175)](https://www.abcam.com/ps/products/170/ab170175/Images/ab170175-169706-ab170175.jpg "Western blot - Anti-Creatine kinase B type antibody [ZN-1] (ab170175)")
Key features and details
- Mouse monoclonal [ZN-1] to Creatine kinase B type
- Suitable for: WB, IP
- Reacts with: Zebrafish
- Isotype: IgG1
Overview
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Product name
Anti-Creatine kinase B type antibody [ZN-1]
See all Creatine kinase B type primary antibodies -
Description
Mouse monoclonal [ZN-1] to Creatine kinase B type -
Host species
Mouse -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Zebrafish
Does not react with: Mouse, Rat, Cow, Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- Brain Homogenate lysates.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purity
SDS-PAGE -
Clonality
Monoclonal -
Clone number
ZN-1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.
Primary overnight incubation. |
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| IP |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Primary overnight incubation. |
|
IP
Use at an assay dependent concentration. |
Target
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Function
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. -
Sequence similarities
Belongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 140744 Zebrafish
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Alternative names
- B CK antibody
- B-CK antibody
- BB-CK antibody
see all
Images
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Western blot - Anti-Creatine kinase B type antibody [ZN-1] (ab170175)All lanes : Anti-Creatine kinase B type antibody [ZN-1] (ab170175) at 2 µg/ml
Lane 1 : ZBH (Zebrafish Brain Homogenate) Lysate
Lane 2 : ZSkMH (Zebrafish Skeletal Muscle Homogenate) Lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG –HRP at 1/1000 dilution
Predicted band size: 43 kDa -
![Immunoprecipitation - Anti-Creatine kinase B type antibody [ZN-1] (ab170175)](https://www.abcam.com/ps/products/170/ab170175/Images/ab170175-169707-ab170175b.jpg)
Immunoprecipitation - Anti-Creatine kinase B type antibody [ZN-1] (ab170175)Immunoprecipitation for Creatine Kinase B type (ZN-1) antibody.
ab170175 conjugated to Protein-G-Beads used at 20 µg antibody per 10 µL of beads. Immunoprecipitated from 1 mg Zebrafish Brain Homogenate Lysate. 25 µL eluate was loaded onto the gel.
Stained with Colloidal Coomassie Blue.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab170175 has not yet been referenced specifically in any publications.
