Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319)
![Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319)](https://www.abcam.com/ps/products/110/ab110319/Images/ab110319-123611-anti-creatine-kinase-mm-antibody-3e5ba5-western-blot.jpg "Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319)")
Key features and details
- Mouse monoclonal [3E5BA5] to Creatine Kinase MM
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Creatine Kinase MM antibody [3E5BA5]
See all Creatine Kinase MM primary antibodies -
Description
Mouse monoclonal [3E5BA5] to Creatine Kinase MM -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human heart homogenate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
ab110319 was produced in vitro using hybridomas grown in serum-free medium. Purity: >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
3E5BA5 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.
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| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. |
Target
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Function
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. -
Sequence similarities
Belongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1158 Human
- Omim: 123310 Human
- SwissProt: P06732 Human
- Unigene: 334347 Human
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Alternative names
- CKM antibody
- CKMM antibody
- Creatine kinase M antibody
see all
Images
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Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319)Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319) at 1 µg/ml + Human heart homogenate at 20 µg
Predicted band size: 43 kDa
Datasheets and documents
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Datasheet download
References (0)
ab110319 has not yet been referenced specifically in any publications.
