Recombinant Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)](https://www.abcam.com/ps/products/275/ab275379/Images/ab275379-4-anti-kat3bp300crebbp-antibody-epr23495276-immunohistochemistry-human-hepatocellular-carcinoma.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23495-276] to CREBBP + KAT3B / p300
- Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] -
Description
Rabbit monoclonal [EPR23495-276] to CREBBP + KAT3B / p300 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-Pmore details
Unsuitable for: ChIP or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, K-562, RAW264.7 and PC-12 whole cell lysates; His-tagged human KAT3B/p300 recombinant protein;His-tagged human CREBBP recombinant protein. IHC-P: Human kidney, colon, clear cell renal carcinoma and hepatocellular carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23495-276 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000. Detects a band of approximately 300 kDa.
|
| Flow Cyt (Intra) |
1/500.
|
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| ICC/IF |
1/100.
|
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| IHC-P |
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 300 kDa. |
|
Flow Cyt (Intra)
1/500. |
|
ICC/IF
1/100. |
|
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Cellular localization
CREBBP: Cytoplasm. Nucleus. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. KAT3B / p300: Cytoplasm. Nucleus. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. Colocalizes with ROCK2 in the nucleus. -
Database links
- Entrez Gene: 1387 Human
- Entrez Gene: 2033 Human
- Entrez Gene: 12914 Mouse
- Entrez Gene: 328572 Mouse
- Entrez Gene: 170915 Rat
- Entrez Gene: 54244 Rat
- Omim: 600140 Human
- Omim: 602700 Human
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling KAT3B/p300+CREBBP with ab275379 at 1/5000 (0.093 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human hepatocellular carcinoma (PMID: 32144235) is observed. The section was incubated with ab275379 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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![Immunocytochemistry/ Immunofluorescence - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)](https://www.abcam.com/ps/products/275/ab275379/Images/ab275379-5-anti-kat3bp300crebbp-antibody-epr23495276-immunocytochemistry-hela.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labelling KAT3B/p300+CREBBP with ab275379 at 1/100 (4.67 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in HeLa cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)](https://www.abcam.com/ps/products/275/ab275379/Images/ab275379-6-anti-kat3bp300crebbp-antibody-epr23495276-flow-cytometry-hela.jpg)
Flow Cytometry (Intracellular) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling KAT3B/p300+CREBBP with ab275379 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)](https://www.abcam.com/ps/products/275/ab275379/Images/ab275379-409476-anti-kat3bp300crebbp-antibody-epr23495276-western-blot-myelogenous.jpg)
Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)All lanes : Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379) at 1/1000 dilution
Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 300 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 8995708).
Lysates were made freshly and used in WB immediately to minimize protein degradation.
Exposure times: Lane 1: 48 seconds; Lane 2: 70 seconds; Lane 3: 59 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)](https://www.abcam.com/ps/products/275/ab275379/Images/ab275379-3-anti-kat3bp300crebbp-antibody-epr23495276-immunohistochemistry-human-clear-cell-renal-cell-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)Immunohistochemical analysis of paraffin-embedded Human clear cell renal cell carcinoma tissue labeling KAT3B/p300+CREBBP with ab275379 at 1/5000 (0.093 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human clear cell renal cell carcinoma (PMID: 28551630). The section was incubated with ab275379 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
-
![Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)](https://www.abcam.com/ps/products/275/ab275379/Images/ab275379-409478-anti-kat3bp300crebbp-antibody-epr23495276-western-blot-recombinant.jpg)
Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)All lanes : Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379) at 1/1000 dilution
Lane 1 : His-tagged human KAT3B/p300 recombinant protein, 20 ng
Lane 2 : His-tagged human CREBBP recombinant protein, E. coli extract, 10 µl
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilutionBlocking and diluting buffer and concentration: 5% NFDM/TBST.
This product can recognize both KAT3B/p300 and CREBBP.
Exposure time: 3 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)](https://www.abcam.com/ps/products/275/ab275379/Images/ab275379-2-anti-kat3bp300crebbp-antibody-epr23495276-immunohistochemistry-human-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling KAT3B/p300+CREBBP with ab275379 at 1/5000 (0.093 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human colon (PMID: 28586030). The section was incubated with ab275379 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
-
![Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)](https://www.abcam.com/ps/products/275/ab275379/Images/ab275379-409477-anti-kat3bp300crebbp-antibody-epr23495276-western-blot-adenocarcinoma.jpg)
Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379) at 1/1000 dilution + HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/5000 dilution
Observed band size: 300 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 8995708).
Lysate was made freshly and used in WB immediately to minimize protein degradation.
Exposure time: 32 seconds.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)](https://www.abcam.com/ps/products/275/ab275379/Images/ab275379-1-anti-kat3bp300crebbp-antibody-epr23495276-immunohistochemistry-human-kidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling KAT3B/p300+CREBBP with ab275379 at 1/5000 (0.093 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human kidney (PMID: 28551630). The section was incubated with ab275379 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
-
![Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)](https://www.abcam.com/ps/products/275/ab275379/Images/ab275379-10-benefits-of-recombinant-antibodies.png)
Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab275379 has not yet been referenced specifically in any publications.