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    products/primary-antibodies/crebbp--kat3b--p300-antibody-epr23495-276-ab275379.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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RecombinantRabMAb

Recombinant Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
  • Immunocytochemistry/ Immunofluorescence - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
  • Flow Cytometry (Intracellular) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
  • Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
  • Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
  • Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
  • Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23495-276] to CREBBP + KAT3B / p300
  • Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276]
  • Description

    Rabbit monoclonal [EPR23495-276] to CREBBP + KAT3B / p300
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-Pmore details
    Unsuitable for: ChIP or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, K-562, RAW264.7 and PC-12 whole cell lysates; His-tagged human KAT3B/p300 recombinant protein;His-tagged human CREBBP recombinant protein. IHC-P: Human kidney, colon, clear cell renal carcinoma and hepatocellular carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23495-276
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Nuclear
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Nuclear
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Rb family
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Obesity

Associated products

  • Alternative Versions

    • Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab275379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000. Detects a band of approximately 300 kDa.
Flow Cyt (Intra)
1/500.
ICC/IF
1/100.
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000. Detects a band of approximately 300 kDa.
Flow Cyt (Intra)
1/500.
ICC/IF
1/100.
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for ChIP or IP.

Target

  • Cellular localization

    CREBBP: Cytoplasm. Nucleus. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. KAT3B / p300: Cytoplasm. Nucleus. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. Colocalizes with ROCK2 in the nucleus.
  • Database links

    • Entrez Gene: 1387 Human
    • Entrez Gene: 2033 Human
    • Entrez Gene: 12914 Mouse
    • Entrez Gene: 328572 Mouse
    • Entrez Gene: 170915 Rat
    • Entrez Gene: 54244 Rat
    • Omim: 600140 Human
    • Omim: 602700 Human
    • SwissProt: Q09472 Human
    • SwissProt: Q92793 Human
    • SwissProt: B2RWS6 Mouse
    • SwissProt: P45481 Mouse
    • SwissProt: Q6JHU9 Rat
    • Unigene: 459759 Human
    • Unigene: 517517 Human
    • Unigene: 655211 Human
    • Unigene: 132238 Mouse
    • Unigene: 258397 Mouse
    • Unigene: 392384 Mouse
    • Unigene: 12815 Rat
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling KAT3B/p300+CREBBP with ab275379 at 1/5000 (0.093 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human hepatocellular carcinoma (PMID: 32144235) is observed. The section was incubated with ab275379 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
    Immunocytochemistry/ Immunofluorescence - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labelling KAT3B/p300+CREBBP with ab275379 at 1/100 (4.67 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in HeLa cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000  dilution.

  • Flow Cytometry (Intracellular) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
    Flow Cytometry (Intracellular) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling KAT3B/p300+CREBBP with ab275379 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). 

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
    Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
    All lanes : Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379) at 1/1000 dilution

    Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 2 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 3 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Observed band size: 300 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 8995708).

    Lysates were made freshly and used in WB immediately to minimize protein degradation.

    Exposure times: Lane 1: 48 seconds; Lane 2: 70 seconds; Lane 3: 59 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)

    Immunohistochemical analysis of paraffin-embedded Human clear cell renal cell carcinoma tissue labeling KAT3B/p300+CREBBP with ab275379 at 1/5000 (0.093 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human clear cell renal cell carcinoma (PMID: 28551630). The section was incubated with ab275379 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
    Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
    All lanes : Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379) at 1/1000 dilution

    Lane 1 : His-tagged human KAT3B/p300 recombinant protein, 20 ng
    Lane 2 : His-tagged human CREBBP recombinant protein, E. coli extract, 10 µl

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution


    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This product can recognize both KAT3B/p300 and CREBBP.

    Exposure time: 3 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling KAT3B/p300+CREBBP with ab275379 at 1/5000 (0.093 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human colon (PMID: 28586030). The section was incubated with ab275379 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
    Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
    Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379) at 1/1000 dilution + HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary
    VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/5000 dilution

    Observed band size: 300 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 8995708).

    Lysate was made freshly and used in WB immediately to minimize protein degradation.

    Exposure time: 32 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling KAT3B/p300+CREBBP with ab275379 at 1/5000 (0.093 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human kidney (PMID: 28551630). The section was incubated with ab275379 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
    Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab275379? Please let us know so that we can cite the reference in this datasheet.

ab275379 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Western blot abreview for Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276]

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Medaka fish Cell lysate - whole cell (Embryo)
Gel Running Conditions
Reduced Denaturing
Loading amount
10000 cells
Specification
Embryo
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Jan 26 2023

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