Recombinant Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23495-276] to CREBBP + KAT3B / p300
- Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] -
Description
Rabbit monoclonal [EPR23495-276] to CREBBP + KAT3B / p300 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-Pmore details
Unsuitable for: ChIP or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, K-562, RAW264.7 and PC-12 whole cell lysates; His-tagged human KAT3B/p300 recombinant protein;His-tagged human CREBBP recombinant protein. IHC-P: Human kidney, colon, clear cell renal carcinoma and hepatocellular carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23495-276 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000. Detects a band of approximately 300 kDa.
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Flow Cyt (Intra) |
1/500.
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ICC/IF |
1/100.
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IHC-P |
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000. Detects a band of approximately 300 kDa. |
Flow Cyt (Intra)
1/500. |
ICC/IF
1/100. |
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Cellular localization
CREBBP: Cytoplasm. Nucleus. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. KAT3B / p300: Cytoplasm. Nucleus. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. Colocalizes with ROCK2 in the nucleus. -
Database links
- Entrez Gene: 1387 Human
- Entrez Gene: 2033 Human
- Entrez Gene: 12914 Mouse
- Entrez Gene: 328572 Mouse
- Entrez Gene: 170915 Rat
- Entrez Gene: 54244 Rat
- Omim: 600140 Human
- Omim: 602700 Human
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling KAT3B/p300+CREBBP with ab275379 at 1/5000 (0.093 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human hepatocellular carcinoma (PMID: 32144235) is observed. The section was incubated with ab275379 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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Immunocytochemistry/ Immunofluorescence - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labelling KAT3B/p300+CREBBP with ab275379 at 1/100 (4.67 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in HeLa cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling KAT3B/p300+CREBBP with ab275379 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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All lanes : Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379) at 1/1000 dilution
Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 300 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 8995708).
Lysates were made freshly and used in WB immediately to minimize protein degradation.
Exposure times: Lane 1: 48 seconds; Lane 2: 70 seconds; Lane 3: 59 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
Immunohistochemical analysis of paraffin-embedded Human clear cell renal cell carcinoma tissue labeling KAT3B/p300+CREBBP with ab275379 at 1/5000 (0.093 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human clear cell renal cell carcinoma (PMID: 28551630). The section was incubated with ab275379 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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All lanes : Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379) at 1/1000 dilution
Lane 1 : His-tagged human KAT3B/p300 recombinant protein, 20 ng
Lane 2 : His-tagged human CREBBP recombinant protein, E. coli extract, 10 µl
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilutionBlocking and diluting buffer and concentration: 5% NFDM/TBST.
This product can recognize both KAT3B/p300 and CREBBP.
Exposure time: 3 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling KAT3B/p300+CREBBP with ab275379 at 1/5000 (0.093 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human colon (PMID: 28586030). The section was incubated with ab275379 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379) at 1/1000 dilution + HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/5000 dilution
Observed band size: 300 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 8995708).
Lysate was made freshly and used in WB immediately to minimize protein degradation.
Exposure time: 32 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling KAT3B/p300+CREBBP with ab275379 at 1/5000 (0.093 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human kidney (PMID: 28551630). The section was incubated with ab275379 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab275379 has not yet been referenced specifically in any publications.