Recombinant Anti-CrkL antibody [Y243] (ab32126)
![Western blot - Anti-CrkL antibody [Y243] (ab32126)](https://www.abcam.com/ps/products/32/ab32126/Images/ab32126-391450-anti-crkl-antibody-y243-western-blot-human-knockout-lysate.jpg "Western blot - Anti-CrkL antibody [Y243] (ab32126)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y243] to CrkL
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CrkL antibody [Y243]
See all CrkL primary antibodies -
Description
Rabbit monoclonal [Y243] to CrkL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa and K562 cell lysates, and human breast carcinoma
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y243 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 33 kDa).
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/50 - 1/100.
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 33 kDa). |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/50 - 1/100. |
Target
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Function
May mediate the transduction of intracellular signals. -
Sequence similarities
Belongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains. - Information by UniProt
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Database links
- Entrez Gene: 1399 Human
- Omim: 602007 Human
- SwissProt: P46109 Human
- Unigene: 5613 Human
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Alternative names
- Crk L antibody
- Crk like protein antibody
- Crk-like protein antibody
see all
Images
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Western blot - Anti-CrkL antibody [Y243] (ab32126)All lanes : Anti-CrkL antibody [Y243] (ab32126) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CRKL knockout HeLa cell lysate
Lane 3 : K-562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 33 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab32126 observed at 37 kDa. Red - loading control ab7291 observed at 50 kDa.
ab32126 Anti-CrkL antibody [Y243] was shown to specifically react with CrkL in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265993 (knockout cell lysate ab257397) was used. Wild-type and CrkL knockout samples were subjected to SDS-PAGE. ab32126 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-CrkL antibody [Y243] (ab32126)](https://www.abcam.com/ps/products/32/ab32126/Images/ab32126-5-anti-crkl-antibody-y243-immunocytochemistry-k562-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CrkL antibody [Y243] (ab32126)Immunocytochemistry analysis of K-562 (human chronic myelogenous leukemia lymphoblast) labeling CrkL with purified ab32126 at 1/100 dilution (10 µg/ml). Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.6 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody. -
![Western blot - Anti-CrkL antibody [Y243] (ab32126)](https://www.abcam.com/ps/products/32/ab32126/Images/ab32126-43968-anti-crkl-antibody-y243-western-blot.jpg)
Western blot - Anti-CrkL antibody [Y243] (ab32126)Anti-CrkL antibody [Y243] (ab32126) at 1/1000 dilution + K562 cell lysate
Predicted band size: 33 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CrkL antibody [Y243] (ab32126)](https://www.abcam.com/ps/products/32/ab32126/Images/ab32126-43967-anti-crkl-antibody-y243-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CrkL antibody [Y243] (ab32126)ab32126, staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-CrkL antibody [Y243] (ab32126)](https://www.abcam.com/ps/products/32/ab32126/Images/ab32126-6-benefits-of-recombinant-antibodies.png)
Anti-CrkL antibody [Y243] (ab32126)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab32126 has been referenced in 5 publications.
- Shi X et al. Downregulated miR-29a promotes B cell overactivation by upregulating Crk-like protein in systemic lupus erythematosus. Mol Med Rep 22:841-849 (2020). PubMed: 32467986
- Wang L et al. Expression of signaling adaptor proteins predicts poor prognosis in pancreatic ductal adenocarcinoma. Diagn Pathol 12:42 (2017). PubMed: 28558797
- Poulsen ET et al. New Insights to Clathrin and Adaptor Protein 2 for the Design and Development of Therapeutic Strategies. Int J Mol Sci 16:29446-53 (2015). IP . PubMed: 26690411
- Wang J et al. SCG10 promotes non-amyloidogenic processing of amyloid precursor protein by facilitating its trafficking to the cell surface. Hum Mol Genet N/A:N/A (2013). PubMed: 23863461
- Natsume H et al. The CRKL gene encoding an adaptor protein is amplified, overexpressed, and a possible therapeutic target in gastric cancer. J Transl Med 10:97 (2012). WB, IHC-P ; Human . PubMed: 22591714