Recombinant Anti-CSF-1-R antibody [EPR21885-161] (ab221684)
![Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR21885-161] (ab221684)](https://www.abcam.com/ps/products/221/ab221684/Images/ab221684-512257-anti-csf-1-r-antibody-epr21885-161-immunocytochemistry-j774a-1-mouse.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR21885-161] (ab221684)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21885-161] to CSF-1-R
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-CSF-1-R antibody [EPR21885-161]
See all CSF-1-R primary antibodies -
Description
Rabbit monoclonal [EPR21885-161] to CSF-1-R -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat lung lysate, Mouse spleen and placenta lysate, J774A.1 and RAW 264.7 whole cell lysate. ICC/IF: J774A.1 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21885-161 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 108 kDa.
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| ICC/IF |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 108 kDa. |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34. -
Tissue specificity
Expressed in bone marrow and in differentiated blood mononuclear cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12978 Mouse
- Entrez Gene: 307403 Rat
- SwissProt: P09581 Mouse
- SwissProt: Q00495 Rat
- Unigene: 22574 Mouse
- Unigene: 72599 Rat
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Alternative names
- C FMS antibody
- CD 115 antibody
- CD115 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR21885-161] (ab221684)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) cells labelling CSF-1-R with ab221684 at 1/50 dilution (8.66 μg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody used at 1/1000 dilution (2µg/ml) (Green). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5µg/ml)(Red). The Nuclear counterstain was DAPI (Blue).
Confocal image showing membranous and cytoplasmic staining in J774A.1 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Western blot - Anti-CSF-1-R antibody [EPR21885-161] (ab221684)](https://www.abcam.com/ps/products/221/ab221684/Images/ab221684-308550-anti-mcsf-receptor-antibody-epr21885-161-western-blot.jpg)
Western blot - Anti-CSF-1-R antibody [EPR21885-161] (ab221684)All lanes : Anti-CSF-1-R antibody [EPR21885-161] (ab221684) at 1/1000 dilution
Lane 1 : Rat lung lysate at 20 µg
Lane 2 : Mouse spleen lysate at 20 µg
Lane 3 : Mouse placenta lysate at 20 µg
Lane 4 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate at 10 µg
Lane 5 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lane 4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution
Predicted band size: 108 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-4: 3 minutes; Lane 5: 32 seconds.
The molecular mass observed is consistent with the literature (PMID 21610095; PMID 20436485; PMID 24362524; PMID: 26066800).
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![Anti-CSF-1-R antibody [EPR21885-161] (ab221684)](https://www.abcam.com/ps/products/221/ab221684/Images/ab221684-2-benefits-of-recombinant-antibodies.png)
Anti-CSF-1-R antibody [EPR21885-161] (ab221684)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab221684 has been referenced in 2 publications.
- Mendel ZI et al. Amino acids suppress macropinocytosis and promote release of CSF1 receptor in macrophages. J Cell Sci 135:N/A (2022). PubMed: 35107133
- Delaney C et al. Attenuated CSF-1R signalling drives cerebrovascular pathology. EMBO Mol Med 13:e12889 (2021). PubMed: 33350588