Anti-CTAG1B antibody [OTI4D8] (ab139339)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [OTI4D8] (ab139339)](https://www.abcam.com/ps/products/139/ab139339/Images/ab139339-154659-anti-ctag1b-antibody-4d8-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [OTI4D8] (ab139339)")
Key features and details
- Mouse monoclonal [OTI4D8] to CTAG1B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-CTAG1B antibody [OTI4D8]
See all CTAG1B primary antibodies -
Description
Mouse monoclonal [OTI4D8] to CTAG1B -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Recombinant full length Human CTAG1B (amino acids 1-180) produced in HEK293T cells.
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Positive control
- HEK293T cell lysate transfected with pCMV6-ENTRY CTAG1B cDNA; COS7 cells transiently transfected with pCMV6-ENTRY CTAG1B cDNA; Human pancreas tissue.
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General notes
Dilute in PBS (pH7.3) before use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, 48% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab139339 is purified from TCS by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI4D8 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab139339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 18 kDa.
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| IHC-P |
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100.
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| Notes |
|---|
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WB
1/2000. Predicted molecular weight: 18 kDa. |
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IHC-P
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100. |
Target
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Tissue specificity
Expressed in testis and ovary and in a wide variety of cancers. Detected in uterine myometrium. Expressed from 18 weeks until birth in human fetal testis. In the adult testis, is strongly expressed in spermatogonia and in primary spermatocytes, but not in post-meiotic cells or in testicular somatic cells (at protein level). -
Sequence similarities
Belongs to the CTAG family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1485 Human
- Entrez Gene: 246100 Human
- Omim: 300156 Human
- SwissProt: P78358 Human
- Unigene: 534310 Human
- Unigene: 729551 Human
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Alternative names
- CTAG1A antibody
- CTAG1B antibody
- Autoimmunogenic cancer/testis antigen NY ESO 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [OTI4D8] (ab139339)Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling CTAG1B with ab139339 at 1/150 dilution.
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![Western blot - Anti-CTAG1B antibody [OTI4D8] (ab139339)](https://www.abcam.com/ps/products/139/ab139339/Images/ab139339-154661-anti-ctag1b-antibody-4d8-western-blot.jpg)
Western blot - Anti-CTAG1B antibody [OTI4D8] (ab139339)All lanes : Anti-CTAG1B antibody [OTI4D8] (ab139339) at 1/2000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CTAG1B cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 18 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC207790) -
![Immunocytochemistry/ Immunofluorescence - Anti-CTAG1B antibody [OTI4D8] (ab139339)](https://www.abcam.com/ps/products/139/ab139339/Images/ab139339-154660-anti-ctag1b-antibody-4d8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CTAG1B antibody [OTI4D8] (ab139339)immunofluorescent analysis of COS7 cells transiently transfected with pCMV6-ENTRY CTAG1B, labelling CTAG1B with ab139339 at 1/100 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab139339 has been referenced in 1 publication.
- Liu MY et al. Cancer stem-like cells with increased expression of NY-ESO-1 initiate breast cancer metastasis. Oncol Lett 18:3664-3672 (2019). PubMed: 31579408