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    products/primary-antibodies/ctcf-antibody-epr7314b-chip-grade-ab128873.pdf

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RecombinantRabMAb

Recombinant Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)

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Western blot - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
  • Western blot - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
  • Western blot - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
  • ChIP - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
  • Western blot - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
  • Immunocytochemistry/ Immunofluorescence - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
  • Immunocytochemistry/ Immunofluorescence - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
  • Flow Cytometry (Intracellular) - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
  • Flow Cytometry (Intracellular) - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
  • ChIC/CUT&RUN sequencing - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
  • ChIP-sequencing - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
  • OI-RD Scanning - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
  • CUT&Tag sequencing - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
  • Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7314(B)] to CTCF - ChIP Grade
  • Suitable for: ChIP-sequencing, ChIP, CUT&Tag-seq, WB, IHC-P, ICC/IF, Flow Cyt (Intra), ChIC/CUT&RUN-seq
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-CTCF antibody [EPR7314(B)] - ChIP Grade
    See all CTCF primary antibodies
  • Description

    Rabbit monoclonal [EPR7314(B)] to CTCF - ChIP Grade
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ChIP-sequencing, ChIP, CUT&Tag-seq, WB, IHC-P, ICC/IF, Flow Cyt (Intra), ChIC/CUT&RUN-seqmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CTCF aa 700-800. The exact sequence is proprietary.
    (Peptide available as ab209492)

  • Positive control

    • WB: HeLa and 293T cell lysates, Human colon tissue, Mouse brain tissue and rat heart tissue. IHC: Human breast carcinoma, mouse and rat kideny ICC/IF: HeLa cell lysates Flow Cyt (intra): 293T cell lysates CUT&Tag-seq: Sox10 chr15:79,091,798-79,329,947 (Mouse mm10 genome used). ChIP-seq: HeLa cells. ChIC/CUT&RUN-seq: HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.74 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.2% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7314(B)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies
    • Epigenetics and Nuclear Signaling
    • DNA methylation
    • Methylated DNA Associated

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-CTCF antibody [EPR7314(B)] (ab203704)
    • Alexa Fluor® 647 Anti-CTCF antibody [EPR7314(B)] (ab203705)
    • Anti-CTCF antibody [EPR7314(B)] - ChIP Grade – BSA and Azide free (ab240035)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • CTCF peptide (ab209492)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CTCF protein (ab153114)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab128873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP-sequencing (1)
Use 4 µg for 30 µg of chromatin.
ChIP
Use at an assay dependent concentration.
CUT&Tag-seq
1/100.
WB
1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 83 kDa).Can be blocked with CTCF peptide (ab209492).
IHC-P
1/250 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Flow Cyt (Intra)
1/40 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ChIC/CUT&RUN-seq
Use 2µg for 105 cells.
Notes
ChIP-sequencing
Use 4 µg for 30 µg of chromatin.
ChIP
Use at an assay dependent concentration.
CUT&Tag-seq
1/100.
WB
1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 83 kDa).Can be blocked with CTCF peptide (ab209492).
IHC-P
1/250 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Flow Cyt (Intra)
1/40 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ChIC/CUT&RUN-seq
Use 2µg for 105 cells.

Target

  • Function

    Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates to the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19.
  • Tissue specificity

    Ubiquitous. Absent in primary spermatocytes.
  • Sequence similarities

    Belongs to the CTCF zinc-finger protein family.
    Contains 11 C2H2-type zinc fingers.
  • Domain

    The 11 zinc fingers are highly conserved among vertebrates, exhibiting almost identical amino acid sequences. Different subsets or combination of individual zinc fingers gives the ability to CTCF to recognize multiple DNA target sites.
  • Post-translational
    modifications

    Sumoylated on Lys-74 and Lys-689; sumoylation of CTCF contributes to the repressive function of CTCF on the MYC P2 promoter.
  • Cellular localization

    Nucleus > nucleoplasm. Chromosome. Chromosome > centromere. May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase.
  • Target information above from: UniProt accession P49711 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10664 Human
    • Entrez Gene: 13018 Mouse
    • Entrez Gene: 83726 Rat
    • Omim: 604167 Human
    • SwissProt: P49711 Human
    • SwissProt: Q61164 Mouse
    • SwissProt: Q9R1D1 Rat
    • Unigene: 368367 Human
    • Unigene: 269474 Mouse
    • Unigene: 18612 Rat
    see all
  • Alternative names

    • 11 zinc finger protein antibody
    • 11 zinc finger transcriptional repressor antibody
    • 11-zinc finger protein antibody
    • CCCTC binding factor (zinc finger protein) antibody
    • CCCTC binding factor antibody
    • CCCTC-binding factor antibody
    • Ctcf antibody
    • CTCF_HUMAN antibody
    • CTCFL paralog antibody
    • MRD21 antibody
    • Transcriptional repressor CTCF antibody
    see all

Images

  • Western blot - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    Western blot - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    All lanes : Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873) at 1/10000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
    Lane 2 : 293T (human embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 83 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer 5% NFDM/TBST
  • Western blot - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    Western blot - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873) at 1/10000 dilution + Mouse brain at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 83 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer 5% NFDM/TBST
  • Western blot - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    Western blot - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873) at 1/10000 dilution + Rat heart at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 83 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer 5% NFDM/TBST
  • ChIP - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    ChIP - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)

    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab128873 (red), and 20µl of protein A/G sepharose beads slurry (10µl of sepharose A beads + 10µl of sepharose G beads). 5μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

  • Western blot - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    Western blot - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    All lanes : Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873) at 1/1000 dilution (un-purified)

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 83 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)

    Immunohistochemical staining of paraffin-embedded human breast carcinoma sections labelling CTCF with purified ab128873 at dilution of 1:1000. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)

    Immunohistochemical staining of paraffin-embedded mouse kidney sections labelling CTCF with purified ab128873 at dilution of 1:1000. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)

    Immunohistochemical staining of paraffin-embedded rat kidney sections labelling CTCF with purified ab128873 at dilution of 1:1000. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labelling CTCF with un-purified ab128873 at 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    Immunocytochemistry/ Immunofluorescence - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)

    Immunocytochemistry/immunofluorescence staining of 4% paraformaldehyde fixed; 0.1% triton X 100 permeabilized HeLa (human cervix adenocarcinoma) cells with purified ab128873 at dilution of 1/500. The secondary antibody used was Alexa Fluor® 488; goat anti-rabbit IgG (ab150077) at a dilution of 1/1000. Nucleus was counter-stained with DAPI (blue). ab7291, a mouse anti-tubulin antibody (1/1000) was used to stain tubulin along with ab150120 (AlexaFluor®594 goat anti-mouse secondary, 1/1000) shown in the top right hand panel. The negative controls are shown in the bottom middle and right hand panels- for negative control 1 rabbit primary antibody and anti-mouse secondary antibody (ab150120) was used. For negative control 2 mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) was used.

  • Immunocytochemistry/ Immunofluorescence - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    Immunocytochemistry/ Immunofluorescence - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CTCF with un-purified ab128873 at 1/250 dilution.

  • Flow Cytometry (Intracellular) - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    Flow Cytometry (Intracellular) - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)

    Flow cytometry analysis showing 4% paraformaldehyde fixed 293T (human embryonic kidney) cells labelling CTCF with purified ab128873 at dilution of 1/40 followed by the secondary antibody; Alexa Fluor® 488 goat-anti-rabbit IgG at dilution of 1/500 (red line). A non-specific IgG antibody (rabbit monoclonal) was used as isotype control (black line). The blue line shows cells without incubation with primary antibody and secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    Flow Cytometry (Intracellular) - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)

    Overlay histogram showing Hela cells stained with un-purified ab128873 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128873, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. 

     

     

  • ChIC/CUT&RUN sequencing - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    ChIC/CUT&RUN sequencing - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)

    CUT&RUN was performed using the ChIC/CUT&RUN pAG-MNAse ab285373, 105 HeLa cells and 2µg of ab128873 [EPR7314(B)]. The resulting DNA was sequenced on the NovaSeq 6000 (PE150).

    Additional screenshots of mapped reads can be downloaded here. 

  • ChIP-sequencing - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    ChIP-sequencing - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 µg of chromatin and 4 µg of ab128873. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Ki Transcription Factors ChIP-Seq (ab270813).

    Additional screenshots of mapped reads can be downloaded here.

  • OI-RD Scanning - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    OI-RD Scanning - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • CUT&Tag sequencing - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    CUT&Tag sequencing - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)This experiment and image is courtesy of Dr Marek Bartosovic, Gonçalo Castelo-Branco Group, Karolinska Institutet.

    CUT&Tag-seq was performed using 200,000 Oli-neu (Oligodendrocyte progenitor) cells.  Cells were permeabilized with 0.05% Digitonin and 0.01% NP-40 for 3 minutes.  A 1:100 dilution of Recombinant Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873) was used, along with a Guinea pig anti-rabbit Secondary.  DNA was seg using Illumina NovaSeq S Prime to a depth of 24 million reads.

     

    This image is courtesy of Dr Marek Bartosovic, Gonçalo Castelo-Branco Group, Karolinska Institutet.

  • Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)
    Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (11)

Publishing research using ab128873? Please let us know so that we can cite the reference in this datasheet.

ab128873 has been referenced in 11 publications.

  • Wang Y  et al. Cardiac-specific LRP6 knockout induces lipid accumulation through Drp1/CPT1b pathway in adult mice. Cell Tissue Res 380:143-153 (2020). PubMed: 31811407
  • Li Y  et al. The structural basis for cohesin-CTCF-anchored loops. Nature 578:472-476 (2020). PubMed: 31905366
  • Olins AL  et al. Hyperosmotic stress: in situ chromatin phase separation. Nucleus 11:1-18 (2020). PubMed: 31924112
  • Su QP  et al. Superresolution imaging reveals spatiotemporal propagation of human replication foci mediated by CTCF-organized chromatin structures. Proc Natl Acad Sci U S A 117:15036-15046 (2020). PubMed: 32541019
  • Ferrari R  et al. TFIIIC Binding to Alu Elements Controls Gene Expression via Chromatin Looping and Histone Acetylation. Mol Cell 77:475-487.e11 (2020). PubMed: 31759822
View all Publications for this product

Customer reviews and Q&As

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ChIP-sequencing abreview for Anti-CTCF antibody [EPR7314(B)] - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
ChIP-sequencing
Sample
Human Cell lysate - nuclear (K562)
Specification
K562
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Andrew Perez

Verified customer

Submitted Dec 01 2021

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