Recombinant Anti-CTHRC1 antibody [EPR22851-145] (ab256458)
![Western blot - Anti-CTHRC1 antibody [EPR22851-145] (ab256458)](https://www.abcam.com/ps/products/256/ab256458/Images/ab256458-353732-anti-cthrc1-antibody-epr22851-145-western-blot-skmel28-human.jpg "Western blot - Anti-CTHRC1 antibody [EPR22851-145] (ab256458)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22851-145] to CTHRC1
- Suitable for: IHC-P, IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CTHRC1 antibody [EPR22851-145]
See all CTHRC1 primary antibodies -
Description
Rabbit monoclonal [EPR22851-145] to CTHRC1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SK-MEL-28 whole cell lysate. IHC-P: Human breast carcinoma, Human colon carcinoma and Human lung carcinoma tissues. IP: SK-MEL-28 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22851-145 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab256458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
1/30.
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| WB |
1/1000. Detects a band of approximately 26, 30 kDa (predicted molecular weight: 26 kDa).
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| Notes |
|---|
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IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/30. |
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WB
1/1000. Detects a band of approximately 26, 30 kDa (predicted molecular weight: 26 kDa). |
Target
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Function
May act as a negative regulator of collagen matrix deposition. -
Tissue specificity
Isoform 1 is expressed in calcified atherosclerotic plaque and chondrocyte-like cells. -
Sequence similarities
Contains 1 collagen-like domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 115908 Human
- Omim: 610635 Human
- SwissProt: Q96CG8 Human
- Unigene: 405614 Human
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Alternative names
- Collagen triple helix repeat containing 1 antibody
- Collagen triple helix repeat containing protein 1 antibody
- Collagen triple helix repeat-containing protein 1 antibody
see all
Images
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Western blot - Anti-CTHRC1 antibody [EPR22851-145] (ab256458)All lanes : Anti-CTHRC1 antibody [EPR22851-145] (ab256458) at 1/1000 dilution
Lane 1 : SK-MEL-28 (human malignant melanoma), whole cell lysate
Lane 2 : SW620 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 26 kDa
Observed band size: 26,30 kDa why is the actual band size different from the predicted?30KDa and 26KDa respectively represent glycosylated and non-glycosylated form. There are three isoforms of CTHRC1. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 26918341).
Negative control: SW620 (PMID: 22321245).
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
Exposure Time: 10 seconds.
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![Immunoprecipitation - Anti-CTHRC1 antibody [EPR22851-145] (ab256458)](https://www.abcam.com/ps/products/256/ab256458/Images/ab256458-4-anti-cthrc1-antibody-epr22851-145-immunoprecipitaion-skmel28-human.jpg)
Immunoprecipitation - Anti-CTHRC1 antibody [EPR22851-145] (ab256458)CTHRC1 was immunoprecipitated from 0.35 mg SK-MEL-28 (Human malignant melanoma) whole cell lysate with ab256458 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256458 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: SK-MEL-28 (Human malignant melanoma) whole cell lysate 10ug
Lane 2: ab256458 IP in SK-MEL-28 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256458 in SK-MEL-28 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] (ab256458)](https://www.abcam.com/ps/products/256/ab256458/Images/ab256458-3-anti-cthrc1-antibody-epr22851-145-immunohistochemistry-lung-and-lung-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] (ab256458)Immunohistochemical analysis of paraffin-embedded Human lung and lung carcinoma tissue labeling CTHRC1 with ab256458 at 1/500 dilution (1.19 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). No staining on the paracancerous human lung tissue (panel A). Positive staining on human lung carcinoma (panel B; PMID: 29631554). The section was incubated with ab256458 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] (ab256458)](https://www.abcam.com/ps/products/256/ab256458/Images/ab256458-2-anti-cthrc1-antibody-epr22851-145-immunohistochemistry-colon-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] (ab256458)Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling CTHRC1 with ab256458 at 1/500 dilution (1.19 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the stromal cells in the human colon carcinoma, nearly no staining on the paracancerous colon tissues (PMID: 24945147). The section was incubated with ab256458 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] (ab256458)](https://www.abcam.com/ps/products/256/ab256458/Images/ab256458-1-anti-cthrc1-antibody-epr22851-145-immunohistochemistry-breast-and-breast-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody [EPR22851-145] (ab256458)Immunohistochemical analysis of paraffin-embedded Human breast and breast carcinoma tissue labeling CTHRC1 with ab256458 at 1/500 dilution (1.19 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). No staining on the paracancerous human breast tissue (panel A). Positive staining on the stromal cells in the human breast carcinoma (panel B; PMID: 24945147). The section was incubated with ab256458 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Anti-CTHRC1 antibody [EPR22851-145] (ab256458)](https://www.abcam.com/ps/products/256/ab256458/Images/ab256458-6-benefits-of-recombinant-antibodies.png)
Anti-CTHRC1 antibody [EPR22851-145] (ab256458)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab256458 has been referenced in 1 publication.
- Zheng Y et al. Let-7c-5p Inhibits Cell Proliferation and Migration and Promotes Apoptosis via the CTHRC1/AKT/ERK Pathway in Esophageal Squamous Cell Carcinoma. Onco Targets Ther 13:11193-11209 (2020). PubMed: 33173311