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    products/primary-antibodies/ctnna1-antibody-ep1793y-ab51032.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CTNNA1 antibody [EP1793Y] (ab51032)

  • Datasheet
  • SDS
Reviews (8)Q&A (3)References (27)

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Immunoprecipitation - Anti-CTNNA1 antibody [EP1793Y] (ab51032)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] (ab51032)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] (ab51032)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] (ab51032)
  • Western blot - Anti-CTNNA1 antibody [EP1793Y] (ab51032)
  • Western blot - Anti-CTNNA1 antibody [EP1793Y] (ab51032)
  • Anti-CTNNA1 antibody [EP1793Y] (ab51032)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1793Y] to CTNNA1
  • Suitable for: WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-CTNNA1 antibody [EP1793Y]
    See all CTNNA1 primary antibodies
  • Description

    Rabbit monoclonal [EP1793Y] to CTNNA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse heart and kidney lysate. Rat brain and kidney lysate. HeLa, A431 and HUVEC whole cell lysate. IHC-P: Rat and human stomach tissue and mouse liver tissue IP: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1793Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Catenins
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins

Associated products

  • Alternative Versions

    • Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa membrane extract lysate (ab29547)
  • Recombinant Protein

    • Recombinant Human CTNNA1 protein (Tagged) (ab51443)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab51032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (4)
1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/100.
Notes
WB
1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/100.
Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Function

    Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
  • Tissue specificity

    Expressed ubiquitously in normal tissues.
  • Sequence similarities

    Belongs to the vinculin/alpha-catenin family.
  • Post-translational
    modifications

    Sumoylated.
  • Cellular localization

    Cell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.
  • Target information above from: UniProt accession P35221 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1495 Human
    • Entrez Gene: 12385 Mouse
    • Entrez Gene: 307505 Rat
    • Omim: 116805 Human
    • SwissProt: P35221 Human
    • SwissProt: P26231 Mouse
    • Unigene: 445981 Human
    • Unigene: 656653 Human
    • Unigene: 18962 Mouse
    see all
  • Alternative names

    • Alpha E-catenin antibody
    • Cadherin associated protein 102kDa antibody
    • Cadherin associated protein antibody
    • Cadherin-associated protein antibody
    • CAP 102 antibody
    • CAP102 antibody
    • Catenin (cadherin associated protein) alpha 1 102kDa antibody
    • Catenin (cadherin associated protein), alpha 1, 102kDa antibody
    • Catenin alpha 1 antibody
    • Catenin alpha-1 antibody
    • CTNA1_HUMAN antibody
    • CTNNA 1 antibody
    • Ctnna1 antibody
    • FLJ36832 antibody
    • FLJ52416 antibody
    • MDPT2 antibody
    • NY REN 13 antigen antibody
    • OTTHUMP00000224141 antibody
    • OTTHUMP00000224147 antibody
    • Renal carcinoma antigen NY REN 13 antibody
    • Renal carcinoma antigen NY-REN-13 antibody
    see all

Images

  • Immunoprecipitation - Anti-CTNNA1 antibody [EP1793Y] (ab51032)
    Immunoprecipitation - Anti-CTNNA1 antibody [EP1793Y] (ab51032)

    ab190685 at 1/100 dilution immunoprecipitating CTNNA1 in Jurkat HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 1 (input): HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
    Lane 2 (+): HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51032 in HeLa whole cell lysate
    For western blotting, ab51032 at 1/500 dilution and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.

    Blocking and diluting buffer: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] (ab51032)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] (ab51032)

    Paraffin-embedded rat stomach tissue stained for CTNNA1 with ab51032 at a 1/100 dilution in immunohistochemical analysis. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as a secondary antibody and Hematoxylin used as a counterstain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 minutes.

    Positive staining was seen on rat stomach.

    The section was incubated with ab51032 for 30 minutes at room temperature.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] (ab51032)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] (ab51032)

    Paraffin-embedded mouse liver tissue stained for CTNNA1 with ab51032 at a 1/100 dilution in immunohistochemical analysis. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as a secondary antibody and Hematoxylin used as a counterstain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 minutes.

    Positive staining was seen on mouse liver.

    The section was incubated with ab51032 for 30 minutes at room temperature.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] (ab51032)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] (ab51032)

    Paraffin-embedded human stomach tissue stained for CTNNA1 with ab51032 at a 1/100 dilution in immunohistochemical analysis. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as a secondary antibody and Hematoxylin used as a counterstain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 minutes.

    Positive staining was seen on human stomach.

    The section was incubated with ab51032 for 30 minutes at room temperature.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-CTNNA1 antibody [EP1793Y] (ab51032)
    Western blot - Anti-CTNNA1 antibody [EP1793Y] (ab51032)
    All lanes : Anti-CTNNA1 antibody [EP1793Y] (ab51032) at 1/10000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat kidney lysate
    Lane 5 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 6 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
    Lane 7 : HUVEC (Human umbilical vein endothelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 100 kDa
    Observed band size: 100 kDa



    Expsure time

    Lane 1-4: 180 seconds

    Lane 5,7: 40 seconds

    Lane 6: 5 seconds

    Blocking/diluting buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-CTNNA1 antibody [EP1793Y] (ab51032)
    Western blot - Anti-CTNNA1 antibody [EP1793Y] (ab51032)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: CTNNA1 HAP1 whole cell lysate (20 µg) 
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HEK293 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab51032 observed at 100 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab51032 was shown to recognize CTNNA1 in wild-type cells as signal was lost at the expected MW in CTNNA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CTNNA1 knockout samples were subjected to SDS-PAGE. Ab51032 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/50000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Anti-CTNNA1 antibody [EP1793Y] (ab51032)
    Anti-CTNNA1 antibody [EP1793Y] (ab51032)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (27)

Publishing research using ab51032? Please let us know so that we can cite the reference in this datasheet.

ab51032 has been referenced in 27 publications.

  • Kilinc AN  et al. Integrin-linked kinase tunes cell-cell and cell-matrix adhesions to regulate the switch between apoptosis and EMT downstream of TGFß1. Mol Biol Cell 32:402-412 (2021). PubMed: 33405954
  • Song J  et al. Apatinib suppresses the migration, invasion and angiogenesis of hepatocellular carcinoma cells by blocking VEGF and PI3K/AKT signaling pathways. Mol Med Rep 23:N/A (2021). PubMed: 33846786
  • Pavel M  et al. a-Catenin levels determine direction of YAP/TAZ response to autophagy perturbation. Nat Commun 12:1703 (2021). PubMed: 33731717
  • Jiang D  et al. Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin. Nat Commun 11:5653 (2020). PubMed: 33159076
  • Zhang X  et al. Wheel running exercise protects against retinal degeneration in the I307N rhodopsin mouse model of inducible autosomal dominant retinitis pigmentosa. Mol Vis 25:462-476 (2019). PubMed: 31523123
View all Publications for this product

Customer reviews and Q&As

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1-10 of 11 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-CTNNA1 antibody [EP1793Y]

Excellent
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Undifferentiated Human ES cells on MEFs)
Permeabilization
Yes - see blocking buffer
Specification
Undifferentiated Human ES cells on MEFs
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 01 2016

Western blot abreview for Anti-CTNNA1 antibody [EP1793Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Undifferentiated Human ES cells on MEFs)
Gel Running Conditions
Non-reduced Denaturing (12.5% SDS gel)
Loading amount
20 µg
Specification
Undifferentiated Human ES cells on MEFs
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 01 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-CTNNA1 antibody [EP1793Y]

Average
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEPG2)
Specification
HEPG2
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 18 2015

Western blot abreview for Anti-CTNNA1 antibody [EP1793Y]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEPG2)
Gel Running Conditions
Reduced Denaturing (12.5)
Loading amount
20 µg
Specification
HEPG2
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 13 2015

Western blot abreview for Anti-CTNNA1 antibody [EP1793Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (hESC, MCF7, MET5A)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
100000 cells
Specification
hESC, MCF7, MET5A
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 18 2015

Flow Cytometry abreview for Anti-CTNNA1 antibody [EP1793Y]

Excellent
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Flow Cytometry
Sample
Mouse Cell (mouse embryonic stem cells)
Permeabilization
Yes - 0.5% v/v tween20 in PBS
Gating Strategy
live cells in fsc vs ssc
Specification
mouse embryonic stem cells
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: 0.5% v/v tween20 in PBS
Fixation
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 30 2014

Western blot abreview for Anti-CTNNA1 antibody [EP1793Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cell)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
1e+006 cells
Specification
mouse embryonic stem cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 30 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-CTNNA1 antibody [EP1793Y]

Excellent
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse embryonic stem cell)
Permeabilization
Yes - 0.1% triton x
Specification
mouse embryonic stem cell
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
Fixative
methanol/acetone 1:1
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 20 2013

Question

It has been tested in mouse rat and human but i would like to test in on Xenopus cells as that is the system we currently work in the lab.

Alpha E-Catenin is pretty well conserved so it is likely to crossreact with Xenopus as well. However, on the datasheet the details about the immunogen are quite vague ("alpha catenin N-terminus") so I was wondering whether it would be possible to have more details about the actual sequence of the immunogen peptide before i take the risk of ordering the antibody.

Alternatively, I was wondering whether it would be possible for me to test the antibody before i buy it or whether i could get a refund in case it does not work. I will be happy to then share the test results with you in case this would be possible.

I look forward to hearing from you,

best regards

Read More

Abcam community

Verified customer

Asked on Apr 17 2013

Answer

Thank you for contacting us.

This product was tested with rat, human and mouse samples only; xenopus was never tried. We can however predict, it will be fine for Xenopus as well because the N-terminal end of Xenopus protein (http://www.uniprot.org/uniprot/F7DHT4) is quite homologous to the human alpha 1 Catenin protein (http://www.uniprot.org/uniprot/P35221).

In case you are interested in trying this antibody then I would encourage you to participate in our 100% Abtrial discount programme - a free product can be claimed in exchange of review for this antibody. Please contact me for code if you accept our offer.

More details about the offer can be found at the following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Apr 17 2013

Question

We are planning to use this antibody for western blot analysis. We are going to analyze both the soluble (cytoplasmic) and insoluble (membrane bound) fractions for the presence of alpha catenin. I was just wondering if you can recommend a molecule that can be used as loading control to analyze the insoluble fraction….something that corresponds to GAPDH or beta-tubulin for cytoplasmic fraction.

Read More

Abcam community

Verified customer

Asked on Jan 13 2012

Answer

Thank you for contacting Abcam. I would recommend ab7671, the alpha 1 Sodium Potassium ATPase antibody as a marker for your membrane fraction. Please let me know if there is anything else I can help you with.

Read More

Abcam Scientific Support

Answered on Jan 13 2012

1-10 of 11 Abreviews or Q&A

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