Recombinant Anti-CTNNBIP1/ICAT antibody [EPR6697(2)] (ab129011)
![Western blot - Anti-CTNNBIP1/ICAT antibody [EPR6697(2)] (ab129011)](https://www.abcam.com/ps/products/129/ab129011/Images/ab129011-142920-anti-ctnnbip1-antibody-epr66972-western-blot.jpg "Western blot - Anti-CTNNBIP1/ICAT antibody [EPR6697(2)] (ab129011)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6697(2)] to CTNNBIP1/ICAT
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-CTNNBIP1/ICAT antibody [EPR6697(2)]
See all CTNNBIP1/ICAT primary antibodies -
Description
Rabbit monoclonal [EPR6697(2)] to CTNNBIP1/ICAT -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CTNNBIP1/ICAT (N terminal). The exact sequence is proprietary.
-
Positive control
- Human placenta, JAR, SW480, 293T and NCCIT cell lysates; permeabilized JAR cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 7.50 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6697(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 9 kDa).
|
| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 9 kDa). |
|
IP
1/10 - 1/100. |
Application notes
Is unsuitable for ICC/IF or IHC-P.
Target
-
Function
Prevents the interaction between CTNNB1 and TCF family members, and acts as negative regulator of the Wnt signaling pathway. -
Sequence similarities
Belongs to the CTNNBIP1 family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 56998 Human
- Omim: 607758 Human
- SwissProt: Q9NSA3 Human
- Unigene: 463759 Human
-
Alternative names
- Beta catenin interacting protein 1 antibody
- Beta catenin interacting protein ICAT antibody
- Beta-catenin-interacting protein 1 antibody
see all
Images
-
Western blot - Anti-CTNNBIP1/ICAT antibody [EPR6697(2)] (ab129011)All lanes : Anti-CTNNBIP1/ICAT antibody [EPR6697(2)] (ab129011) at 1/1000 dilution
Lane 1 : Human placenta cell lysate
Lane 2 : JAR cell lysate
Lane 3 : SW480 cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 5 : NCCIT cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 9 kDa -
![Flow Cytometry (Intracellular) - Anti-CTNNBIP1/ICAT antibody [EPR6697(2)] (ab129011)](https://www.abcam.com/ps/products/129/ab129011/Images/ab129011-142919-anti-ctnnbip1-antibody-epr66972-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-CTNNBIP1/ICAT antibody [EPR6697(2)] (ab129011)ab129011, at 1/10 diltuion, staining CTNNBIP1/ICAT in permeabilized JAR cells by intracellular flow cytometry (red) or Rabbit IgG negative control (green).
-
![OI-RD Scanning - Anti-CTNNBIP1/ICAT antibody [EPR6697(2)] (ab129011)](https://www.abcam.com/ps/products/129/ab129011/Images/ab129011-142918-anti-ctnnbip1-antibody-epr66972.jpg)
OI-RD Scanning - Anti-CTNNBIP1/ICAT antibody [EPR6697(2)] (ab129011) -
![Anti-CTNNBIP1/ICAT antibody [EPR6697(2)] (ab129011)](https://www.abcam.com/ps/products/129/ab129011/Images/ab129011-4-benefits-of-recombinant-antibodies.png)
Anti-CTNNBIP1/ICAT antibody [EPR6697(2)] (ab129011)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab129011 has been referenced in 6 publications.
- Wang Z et al. ICAT promotes colorectal cancer metastasis via binding to JUP and activating the NF-κB signaling pathway. J Clin Lab Anal 36:e24678 (2022). PubMed: 36036768
- Ou MY et al. The CTNNBIP1-CLSTN1 fusion transcript regulates human neocortical development. Cell Rep 35:109290 (2021). PubMed: 34192541
- Zhou J et al. miR-296-3p promotes the proliferation of glioblastoma cells by targeting ICAT. Mol Med Rep 21:2151-2161 (2020). PubMed: 32323769
- Zhao X et al. Long noncoding RNA CA7-4 promotes autophagy and apoptosis via sponging MIR877-3P and MIR5680 in high glucose-induced vascular endothelial cells. Autophagy 16:70-85 (2020). PubMed: 30957640
- Fu X et al. Linc00210 drives Wnt/ß-catenin signaling activation and liver tumor progression through CTNNBIP1-dependent manner. Mol Cancer 17:73 (2018). PubMed: 29540185
- Li X et al. SAMMSON drives the self-renewal of liver tumor initiating cells through EZH2-dependent Wnt/ß-catenin activation. Oncotarget 8:103785-103796 (2017). PubMed: 29262600