Recombinant Anti-CUTC antibody [EPR7929] (ab133762)
![Western blot - Anti-CUTC antibody [EPR7929] (ab133762)](https://www.abcam.com/ps/products/133/ab133762/Images/ab133762-410250-anti-cutc-antibody-epr7929-western-blot-human-knockout-lysate.jpg "Western blot - Anti-CUTC antibody [EPR7929] (ab133762)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7929] to CUTC
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CUTC antibody [EPR7929]
See all CUTC primary antibodies -
Description
Rabbit monoclonal [EPR7929] to CUTC -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SH-SY5Y, Jurkat, HeLa and A431 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Dissociation constant (KD)
KD = 2.00 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7929 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
Target
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Function
May play a role in copper homeostasis. Can bind one Cu(1+) per subunit. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the CutC family. -
Cellular localization
Cytoplasm. Nucleus. The overexpressed protein is detected in the cytoplasm, and depending on the cell line, also in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 51076 Human
- Entrez Gene: 66388 Mouse
- Entrez Gene: 361760 Rat
- Omim: 610101 Human
- SwissProt: Q9NTM9 Human
- SwissProt: Q9D8X1 Mouse
- Unigene: 16606 Human
- Unigene: 20257 Mouse
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Alternative names
- CGI 32 antibody
- CGI32 antibody
- Copper homeostasis protein cutC homolog antibody
see all
Images
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Western blot - Anti-CUTC antibody [EPR7929] (ab133762)All lanes : Anti-CUTC antibody [EPR7929] (ab133762) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CUTC knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDaLanes 1-5: Merged signal (red and green). Green - ab133762 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.
ab133762 Anti-CUTC antibody [EPR7929] was shown to specifically react with CUTC in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265860 (knockout cell lysate ab258388) was used. Wild-type and CUTC knockout samples were subjected to SDS-PAGE. ab133762 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-CUTC antibody [EPR7929] (ab133762)](https://www.abcam.com/ps/products/133/ab133762/Images/CUTC-Primary-antibodies-ab133762-1.jpg)
Western blot - Anti-CUTC antibody [EPR7929] (ab133762)All lanes : Anti-CUTC antibody [EPR7929] (ab133762) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa -
![OI-RD Scanning - Anti-CUTC antibody [EPR7929] (ab133762)](https://www.abcam.com/ps/products/133/ab133762/Images/CUTC-Primary-antibodies-ab133762-2.jpg)
OI-RD Scanning - Anti-CUTC antibody [EPR7929] (ab133762) -
![Anti-CUTC antibody [EPR7929] (ab133762)](https://www.abcam.com/ps/products/133/ab133762/Images/ab133762-4-benefits-of-recombinant-antibodies.png)
Anti-CUTC antibody [EPR7929] (ab133762)
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab133762 has not yet been referenced specifically in any publications.