Anti-CX3CR1 antibody [8E10.D9] (ab184678)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CR1 antibody [8E10.D9] (ab184678)](https://www.abcam.com/ps/products/184/ab184678/Images/ab184678-215637-anti-cx3cr1-antibody-8e10d9-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CR1 antibody [8E10.D9] (ab184678)")
Key features and details
- Mouse monoclonal [8E10.D9] to CX3CR1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CX3CR1 antibody [8E10.D9]
See all CX3CR1 primary antibodies -
Description
Mouse monoclonal [8E10.D9] to CX3CR1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Cow
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Immunogen
Synthetic peptide corresponding to Human CX3CR1 aa 200-300 conjugated to keyhole limpet haemocyanin.
Database link: P49238 -
Positive control
- Human brain tissue; Human spleen lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
8E10.D9 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1. -
Tissue specificity
Expressed in lymphoid and neural tissues. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 100124525 Cow
- Entrez Gene: 1524 Human
- Entrez Gene: 13051 Mouse
- Entrez Gene: 100008874 Rabbit
- Omim: 601470 Human
- SwissProt: A6QNL7 Cow
- SwissProt: P49238 Human
- SwissProt: Q9Z0D9 Mouse
see all -
Alternative names
- Beta chemokine receptor-like 1 antibody
- C X3 C CKR 1 antibody
- C-X3-C CKR-1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CR1 antibody [8E10.D9] (ab184678)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling CX3CR1 with ab184678 at 1/50 dilution.
Datasheets and documents
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Datasheet download
References (5)
ab184678 has been referenced in 5 publications.
- Kawamura N et al. Impaired brain fractalkine-CX3CR1 signaling is implicated in cognitive dysfunction in diet-induced obese mice. BMJ Open Diabetes Res Care 9:N/A (2021). PubMed: 33568358
- Wojdasiewicz P et al. Comparative Analysis of the Occurrence and Role of CX3CL1 (Fractalkine) and Its Receptor CX3CR1 in Hemophilic Arthropathy and Osteoarthritis. J Immunol Res 2020:2932696 (2020). PubMed: 32884948
- Abu El-Asrar AM et al. Evaluation of Proteoforms of the Transmembrane Chemokines CXCL16 and CX3CL1, Their Receptors, and Their Processing Metalloproteinases ADAM10 and ADAM17 in Proliferative Diabetic Retinopathy. Front Immunol 11:601639 (2020). PubMed: 33552057
- Kawamura N et al. Reduced brain fractalkine-CX3CR1 signaling is involved in the impaired cognition of streptozotocin-treated mice. IBRO Rep 9:233-240 (2020). PubMed: 32995659
- Falkenham A et al. Nonclassical resident macrophages are important determinants in the development of myocardial fibrosis. Am J Pathol 185:927-42 (2015). PubMed: 25794704
