Anti-CX3CR1 antibody (ab8020)
Key features and details
- Rabbit polyclonal to CX3CR1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CX3CR1 antibody
See all CX3CR1 primary antibodies -
Description
Rabbit polyclonal to CX3CR1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide, corresponding to amino acids 175-189 of Human CX3CR 1.
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Positive control
- THP-1 cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 10 µg/ml.
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| WB | (3) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
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| Notes |
|---|
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IHC-P
Use a concentration of 10 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. |
Target
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Function
Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1. -
Tissue specificity
Expressed in lymphoid and neural tissues. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1524 Human
- Entrez Gene: 13051 Mouse
- Entrez Gene: 171056 Rat
- Omim: 601470 Human
- SwissProt: P49238 Human
- SwissProt: Q9Z0D9 Mouse
- SwissProt: P35411 Rat
- Unigene: 78913 Human
see all -
Alternative names
- Beta chemokine receptor-like 1 antibody
- C X3 C CKR 1 antibody
- C-X3-C CKR-1 antibody
see all
Images
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Western blot - Anti-CX3CR1 antibody (ab8020)All lanes : Anti-CX3CR1 antibody (ab8020) at 1 µg/ml
Lane 1 : Wild-type THP-1 cell lysate
Lane 2 : CX3CR1 knockout THP-1 cell lysate
Lane 3 : Human Spleen cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-CX3CR1 antibody staining at 1 ug/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab8020 was shown to bind specifically to CX3CR1. A band was observed at 42 kDa in wild-type THP-1 cell lysates with no signal observed at this size in CX3CR1 knockout cell line ab273713 (knockout cell lysate ab275510). To generate this image, wild-type and CX3CR1 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CR1 antibody (ab8020)ab8020 at 10µg/ml staining CX3CR1 in human spleen cells by IHC
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Western blot - Anti-CX3CR1 antibody (ab8020)All lanes : Anti-CX3CR1 antibody (ab8020) at 1/500 dilution
Lane 1 : THP-1 cell lysate with absence of blocking peptide
Lane 2 : THP-1 cell lysate with presence of blocking peptide
Predicted band size: 50 kDa
Observed band size: 50 kDa
Protocols
Datasheets and documents
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Datasheet download
References (14)
ab8020 has been referenced in 14 publications.
- Chen X et al. CX3CL1/CX3CR1 axis attenuates early brain injury via promoting the delivery of exosomal microRNA-124 from neuron to microglia after subarachnoid hemorrhage. J Neuroinflammation 17:209 (2020). PubMed: 32664984
- Chrobok NL et al. Tissue Transglutaminase Appears in Monocytes and Macrophages but Not in Lymphocytes in White Matter Multiple Sclerosis Lesions. J Neuropathol Exp Neurol 78:492-500 (2019). PubMed: 31058279
- Szukiewicz D et al. Anti-inflammatory Action of Metformin with Respect to CX3CL1/CX3CR1 Signaling in Human Placental Circulation in Normal-Glucose Versus High-Glucose Environments. Inflammation 41:2246-2264 (2018). PubMed: 30097812
- Löfroos AB et al. Colorectal cancer-infiltrating T lymphocytes display a distinct chemokine receptor expression profile. Eur J Med Res 22:40 (2017). Flow Cyt ; Human . PubMed: 29020986
- Szukiewicz D et al. High Glucose Level Disturbs the Resveratrol-Evoked Curtailment of CX3CL1/CX3CR1 Signaling in Human Placental Circulation. Mediators Inflamm 2017:9853108 (2017). IHC ; Human . PubMed: 28655972
