Recombinant Anti-CX3CR1 antibody [EPR24267-2] - BSA and Azide free (ab308614)

This data was developed using 308613, the same antibody clone in a different buffer formulation. 

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Negative control: Skeletal muscle(PMID: 12881312).

Lanes 4-5 of the blot were freshly made and used for Western blotting immediately to minimize protein degradation.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

Exposure time: 180 seconds

This data was developed using AB308613, the same antibody clone in a different buffer formulation.

CX3CR1 was immunoprecipitated from 0.35 mg Mouse spinal cord tissue lysate with AB308613 at 1/30 dilution (2ug in 0.35mg lysates).

Western blot was performed on the immunoprecipitate using AB308613 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Mouse spinal cord tissue lysate 10 ug

Lane 2: abAB308613 IP in Mouse spinal cord tissue lysate

Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab308613 in Mouse spinal cord tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 41 seconds

Input is non-boiled as boiling may cause protein aggregates.

This data was developed using AB308613, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling CX3CR1 with AB308613 at 1/1000 (0.479 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on microglial cells of mouse cerebrum. The section was incubated with ab308613 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

This data was developed using AB308613, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling CX3CR1 with AB308613 at 1/1000 (0.479 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on immune cells of mouse lung. The section was incubated with ab308613 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

This data was developed using AB308613, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse breast cancer tissue labeling CX3CR1 with AB308613 at 1/1000 (0.479 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on immune cells of mouse breast cancer. The section was incubated with ab308613 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

This data was developed using AB308613, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling CX3CR1 with AB308613 at 1/1000 (0.479 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on mouse skeletal muscle. The section was incubated with ab308613 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.