Recombinant Anti-CXCL1/GRO alpha antibody [EPR19892] - BSA and Azide free (ab251456)
![Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] - BSA and Azide free (ab251456)](https://www.abcam.com/ps/products/251/ab251456/Images/ab251456-477315-anti-cxcl1gro-alpha-antibody-epr19892-bsa-and-azide-free-western-blot-wildtype-hela-treated.jpg "Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] - BSA and Azide free (ab251456)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19892] to CXCL1/GRO alpha - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-CXCL1/GRO alpha antibody [EPR19892] - BSA and Azide free
See all CXCL1/GRO alpha primary antibodies -
Description
Rabbit monoclonal [EPR19892] to CXCL1/GRO alpha - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody cross-reacts with recombinant human CXCL2 and CXCL3 protein.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251456 is the carrier-free version of ab206411.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19892 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
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| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). |
Target
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Function
Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsN-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2919 Human
- Omim: 155730 Human
- SwissProt: P09341 Human
- Unigene: 708652 Human
- Unigene: 789 Human
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Alternative names
- C-X-C motif chemokine 1 antibody
- Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) antibody
- chemokine (C-X-C motif) ligand 1 antibody
see all
Images
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Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] - BSA and Azide free (ab251456)All lanes : Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411) at 1/100 dilution
Lane 1 : Wild-type HeLa Treated TNF alpha (5 ng/mL, 6 h) + BFA (5 ug/mL, 6 h) cell lysate
Lane 2 : CXCL1 knockout HeLa Treated TNF alpha (5 ng/mL, 6 h) + BFA (5 ug/mL, 6 h) cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 8 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-CXCL1/GRO alpha antibody [EPR19892] staining at 1/100 dilution, shown in black; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab206411 was shown to bind specifically to CXCL1/GRO alpha. A band was observed at 8 kDa in treated wild-type HeLa cell lysates with no signal observed at this size in CXCL1 knockout cell line ab261774 (knockout cell lysate ab257079). To generate this image, wild-type and CXCL1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 20 minutes exposure time. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and HRP conjugated Goat anti-Mouse (H+L) at 1/20000 dilution.
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![Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] - BSA and Azide free (ab251456)](https://www.abcam.com/ps/products/251/ab251456/Images/ab251456-403387-anti-cxcl1-gro-alpha-antibody-epr19892-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] - BSA and Azide free (ab251456)All lanes : Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411) at 1/1000 dilution
Lane 1 : Human CXCL1/GRO alpha active protein
Lane 2 : Human GRO beta fragment recombinant protein
Lane 3 : Human GRO gamma fragment recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 11 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab206411, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Human GRO beta fragment recombinant protein contain aa35-107 with a GST-Tag. Human GRO gamma fragment recombinant protein contain aa35-107 with a His-Tag®. These two protein were made in house.
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![Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] - BSA and Azide free (ab251456)](https://www.abcam.com/ps/products/251/ab251456/Images/ab251456-399209-anti-cxcl1-gro-alpha-antibody-epr19892-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] - BSA and Azide free (ab251456)Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411) at 1/100 dilution + Human fetal lung lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 3 minutesThis data was developed using ab206411, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-CXCL1/GRO alpha antibody [EPR19892] - BSA and Azide free (ab251456)](https://www.abcam.com/ps/products/251/ab251456/Images/ab251456-3-benefits-of-recombinant-antibodies.png)
Anti-CXCL1/GRO alpha antibody [EPR19892] - BSA and Azide free (ab251456)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab251456 has not yet been referenced specifically in any publications.
