Anti-Cy3 / Cy5 antibody [CY96] (ab52060)
Key features and details
- Mouse monoclonal [CY96] to Cy3 / Cy5
- Suitable for: Dot blot, ELISA, IP, ICC, ICC/IF
- Reacts with: Species independent
- Isotype: IgG2a
Overview
-
Product name
Anti-Cy3 / Cy5 antibody [CY96] -
Description
Mouse monoclonal [CY96] to Cy3 / Cy5 -
Host species
Mouse -
Tested applications
Suitable for: Dot blot, ELISA, IP, ICC, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
A mixture of proteins labelled with Cy3 or Cy5.
-
General notes
Monoclonal antibodies to Cy3 and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purification notes
Purified Mouse Immunoglobulin -
Primary antibody notes
Monoclonal antibodies to Cy3 and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5. -
Clonality
Monoclonal -
Clone number
CY96 -
Isotype
IgG2a -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot |
Use a concentration of 1 - 2 µg/ml.
|
|
| ELISA |
Use at an assay dependent concentration.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| ICC |
Use at an assay dependent concentration.
|
|
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Dot blot
Use a concentration of 1 - 2 µg/ml. |
|
ELISA
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
|
ICC
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
Target
-
Relevance
Cy3 and Cy5 are reactive watersoluble fluorescent dyes of the cyanine dye family used for labelling proteins, peptides, DNA, RNA, and other biomolecules. They are usually synthesized with reactive groups on either one or both of the nitrogen side chains so that they can be chemically cross-linked to nucleic acids or protein molecules. Thay are small, pH insensitive (between pH 3 and pH 10), soluble in aqueous solution and have tolerance to DMSO. They are more photostable than fluorescein, have high molar extinction coefficients and favorable quantum yields. They are used in many different biological assays, including DNA microarrays, protein microarrays, two-dimensional protein analysis, fluorescence resonance energy transfer, and immunocytochemistry. Cy3 is excited maximally at 550 nm and emits maximally at 570 nm, in the green part of the spectrum. Cy5 is excited maximally at 649 nm and emits maximally at 670 nm, in the red part of the spectrum.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab52060 has been referenced in 6 publications.
- Wang C et al. RasGRP1 promotes the acute inflammatory response and restricts inflammation-associated cancer cell growth. Nat Commun 13:7001 (2022). PubMed: 36385095
- Rodriguez FJ et al. Alternative lengthening of telomeres, ATRX loss and H3-K27M mutations in histologically defined pilocytic astrocytoma with anaplasia. Brain Pathol 29:126-140 (2019). PubMed: 30192422
- Rodriguez FJ et al. Telomere alterations in neurofibromatosis type 1-associated solid tumors. Acta Neuropathol Commun 7:139 (2019). PubMed: 31462295
- Palsgrove DN et al. Subependymal giant cell astrocytoma-like astrocytoma: a neoplasm with a distinct phenotype and frequent neurofibromatosis type-1-association. Mod Pathol N/A:N/A (2018). PubMed: 29973652
- Osterwald S et al. PML induces compaction, TRF2 depletion and DNA damage signaling at telomeres and promotes their alternative lengthening. J Cell Sci 128:1887-900 (2015). PubMed: 25908860
- Jeffery R et al. Identifying the origin and phenotype of cells in tumor xenografts. Methods Mol Biol 1070:235-45 (2014). PubMed: 24092445